EP0909952A2 Expired / Lapsed EP ⏱️ 0.0 years remaining

Biosensor and method for quantitative measurement of a substrate using the same

Assignee: Panasonic Holdings Corp

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Filing: 1998-09-25

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Granted: —

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Expires: 2018-09-25

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Abstract

Abstract A biosensor is disclosed for application to a method for quantitative measurement of a substrate comprising a first step of causing a substrate contained in a sample to react with an oxidoreductase specific to the substrate in the presence of an electron mediator in oxidized state, a second step of applying a potential to a working electrode for reducing the electron mediator in oxidized state that remains not reduced in the first step, and a third step of measuring a reduction current flowing across the working electrode and a counter electrode. The biosensor comprises an electrically insulating base plate, an electrode system having a working electrode and a counter electrode formed on the base plate, and a reaction layer which is formed on or in the vicinity of the electrode system and contains at least an oxidoreductase and an electron mediator, the counter electrode including at least a reductant of a redox compound or an electrolytically oxidizable metal.

Inventors

👤 Shin Ikeda
👤 Toshihiko Yoshioka
👤 Shiro Nankai

Bibliographic Details

Application Number —

Jurisdiction EP

Data Source google_patents

Last Synchronized 2026-11-05 04:31

CPC Classifications

C; C12; C12Q; C12Q1/00; C12Q1/001; C12Q1/004; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54366; G01N33/54373; G01N33/5438; Y; Y10; Y10S; Y10S435/00; Y10S435/817

Claims Analysis

Total Claims: 6

Claim 1

Independent Claim 68 words

A biosensor comprising an electrically insulating base plate, an electrode system having a working electrode and a counter electrode formed on said base plate, and a reaction layer which is formed on or in the vicinity of said electrode system and contains at least an oxidoreductase and an electron mediator, said counter electrode including at least a reductant of a redox compound or a metal permitting electrolytic oxidation.

Claim 2

Dependent Claim 17 words

The biosensor in accordance with claim 1, wherein said redox compound is ferrocene or a ferrocene derivative.

Claim 3

Dependent Claim 25 words

The biosensor in accordance with claim 1, wherein said counter electrode is composed of a mixture of at least one electrolytically oxidizable metal with carbon.

Claim 4

Dependent Claim 16 words

The biosensor in accordance with claim 1, wherein said electrolytically oxidizable metal is silver or copper.

Claim 5

Dependent Claim 16 words

The biosensor in accordance with claim 1, wherein said reaction layer further contains a hydrophilic polymer.

Claim 6

Independent Claim 157 words

A method for quantitative measurement of a substrate by using a biosensor which comprises an electrically insulating base plate, an electrode system having a working electrode and a counter electrode including at least a reductant of a redox compound and an electrically oxidizable metal, both being formed on said base plate, and a reaction layer which is formed on or in the vicinity of said electrode system and contains at least an oxidoreductase and an electron mediator, said method comprising: a first step of adding a sample to said reaction layer to cause a substrate contained in said sample to react with an enzyme contained in said reaction layer, a second step of applying a potential to said working electrode for reducing said electron mediator in oxidized state that remains not reduced in the course of said first step, and a third step of measuring a reduction current flowing across said working electrode and said counter electrode.

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