Stabilized immune complex and its use

Abstract

Abstract PURPOSE:The titled complex in a solid phase obtained by supporting a substance such as protein A, etc. to be linked specifically to a Fc part of immunoglobulin on fine powder of a carrier to give a bonded substance, linking the bonded substance to an immunoglobulin molecule, which is a specific antibody. CONSTITUTION:An Fc part of an immunoglobulin molecule constituting an antibody corresponding to an antigen to be measured is linked to bonded protein A-sephalose CL4B on which a substance such as protein A, etc. is supported on fine powder of a carrier (e.g., particles insoluble in aqueous medium: polystyrene, silica, metallic powder, staphylococcus, etc.), to give a stabilized immune complex in a solid state wherein the Fc part is masked specifically and Fab or Fd site, a site of a specific antigen bond, remains in a free type. EFFECT:In the complex, an extremely small amount of antigen and antibody can be measured by a simple operation, and, especially in the antibody, anti-D activity can be measured in a level classified by class, subclass, and type of the immunoglobulin constituting the antibody.

Inventors

• 👤 Yoshitake Terano
• 👤 由剛 寺野
• 👤 Makoto Hirai
• 👤 誠 平井

Bibliographic Details