Light source device for irradiating cell in automatic analysis instrument for cell

Abstract

Abstract PURPOSE:To enable high-brightness excitation and to obtain high fluorescent sensitivity by lighting a flash lamp of a light source for excitation of fluorescence when the arrival of a cell at a measuring position is detected. CONSTITUTION:The beam from a semiconductor laser 3 irradiates continuously the measuring position P and the cells in the coaxial laminar flow in a flow cell 1 flows by each piece downward by passing through the position P. A forward scattering signal S1 and a 90 deg. scattering signal S2 increase instantaneously when the cell passes through the position P. The information on the size of the cell is obtd. by the signal S1 and the information on the internal structure of the cell is obtd. by the signal S2. A cell detecting circuit 29 monitors at all times the signal S1 and outputs the cell detection signal when the S1 exceeds a trigger level. A xenon flash lamp 14 is instantaneously lighted by the signal. The fluorescence emitted from the cell enters a photomultiplier 25 by passing through a fluorescence selecting filter 24 and generates a fluorescence signal S3.

Inventors

• 👤 Kiyoshi Hattori
• 👤 清 服部
• 👤 Taichiro Nishiyama
• 👤 太一郎 西山
• 👤 Fumihiko Sakai
• 👤 文彦 酒井

Bibliographic Details