Reagent for measuring reticulocyte for flow sightmetry

Abstract

Abstract PURPOSE:To prevent the decrease of fluorescent dye Auramine O by hydrolysis by dividing a reagent to two liquids; a dye dissolved in a nonaq. solvent and aq. buffer soln. of an optimum staining condition. CONSTITUTION:This reagent consists of the two liquids; the dye raw liquid dissolved in the nonaq. solvent and the buffer soln. of the optimum staining condition. The dye raw liquid consists of the Auramine O and the nonaq. solvent and alcohols are preferable as the solvent. The buffer soln. contains a buffer and osmotic pressure compensating agent. The buffer is used to maintain the pH of the dyeing liquid and the osmotic pressure compensating agent is used to suppress the deformation of the blood cells. Since the dye Auramine O is preserved in the nonaq. solvent till just before the measurement, the deposition of the Michler's ketone which adversely affects the measurement of the reticulocyte is obviated and the decrease of the Auramine O during the preservation is obviated as well. The always stable final dyeing liquid for measurement of the reticulocyte is obtd. by mixing the two liquids just before the measurement.

Inventors

• 👤 Tomoyuki Kuroda

Bibliographic Details