Urinary estrogen compositions and methods for preparing them

Claim 1

THE PROCESS FOR OBTAINING AN ESTROGENIC, THERAPEUTIC PRODUCT COMPRISING EXTRACTING EQUINE URINARY MATERIAL CONTAINING WATER-SOLUBLE ESTROGENIC SUBSTANCES IN CONJUGATE FROM WITH AN ORGANIC SOLVENT SELECTED FROM THE GROUP CONSISTING OF ALIPHATIC KETONES AND ALCOHOLS HAVING A WATER-SOLUBILITY OF LESS THAN ABOUT 6% WHICH SELECTIVELY REMOVES SUBSTANTIALLY ALL OF THE ESTROGENIC CONJUGATES TOGETHER WITH SOME URINARY SOLIDS FROM PROTEINACEOUS MATERIALS AS WELL AS HIGHER ALIPHATIC AMINES AND DIAMINES, WASHING UNDER ACID CONDITIONS THE EXTRACT OBTAINED, WITH BENZENE, AND THEN WITH ETHER TO REMOVE PHENOLIC CONSTITUENTS THUS OBTAINING AN ESTROGENIC CONCENTRATE SUBSTANTIALLY FREE OF ODORIFEROUS URINARY SUBSTANCES, NEUTRALIZING THE ACIDITY, SEPARATING THE ACTIVE ESTROGENIC COMPONENTS SUBSTANTIALLY FREE OF PYROGENS, INDOXYL AND CRESOL TOXIC COMPOUNDS BY CONTACTING SAID CONCENTRATE WITH A SATURATED AQUEOUS SOLUTION OF AN ALKALI METAL SALT OF AN ACID OF THE GROUP CONSISTING OF SULFURIC, PHOSPHORIC AND HYDROCHLORIC ACID, SAID SALT SOLUTION CAUSING THE PRECIPITATION OF THE ESTROGENIC FACTORS SUBSTANTIALLY FREE OF SAID TOXIC COMPONENTS, THEN EXTRACTING THE ESTROGENIC COMPONENTS FROM SAID PRECIPITATED MATERIAL WITH A MIXTURE OF LOWER ALIPHATIC KETONE AND ALCOHOL SOLVENT AND FINALLY EVAPORATING OFF THE SOLVENT TO OBTAIN THE ESTROGENIC POTENCY IN HIGHLY PURIFIED FROM.