Method of producing and purifying a new proteolytic enzyme

Claim 1

THE CRYSTALLINE PROTEASE OBTAINED FROM WHEAT BRAN CULTURES OF A BLACK ASPERGILLUS TYPE MOLD SELECTED FROM THE GROUP CONSISTING OF ASPERGILLUS SAITOI, ASPERGILLUS USAMII, AND ASPERGILLUS AWAMORI, SAID POTEASE FORMING PLATE-SHAPED TO NEEDLE-SHAPED CRYSTALS WHICH HAVE A MOLECULAR WEIGHT RANGING FROM ABOUT 93,000 TO 98,000, ARE ACID-STABLE, HAVE AN ISOELECTRI POINT OF 4.50 TO 4.70 EXHIBIT THEIR GREATEST ACTIVITY BETWEEN PH 2.5 AND 3.0, HAVE AN OPTIMUM PH FOR MILK CASEIN DIGESTION BETWEEN 2.7 AND 2.9 AND PROCESS A PROTEIN UNIT OF 6700 PU/MG. PROTEIN-N.

Claim 3

THE METHOD OF OBTAINING A CRYSTALLINE PROTEASE FROM A MOLD OF THE BLACK ASPERGILLUS TYPE SELECTED FROM THE GROUP CONSISTING OF ASPERGILLUS SAITOI, ASPERGILLUS USAMI, AND ASPERGILLUS AWAMORI, WHICH COMPRISES THE STEPS OD INOCULATING A NUTRIENT MEDIUM WITH THE ASPERGILLUS INCUBATING AT ORDINARY TEMPERATURE, EXTRACTING WITH WATER AT A PH OF ABOUT 2.5 TO 3.0, ADDING ETHYL ALCOHOL AND STARCH TO THE EXTRACT, REMOVING EXCESS STARCH, PRECIPITATING THE EXTRACT WITH ADDITIONAL ETHYL ALCOHOL, DISSOLVING THE PRECIPITATE IN AN ACETATE TYPE BUFFER AT PH OF ABOUT 3.0 ADDING CALCIUM ACETATE TO REMOVE IMPURITIER, DIALYZING THE SOLUTION TO REMOVE IMPURITIES, ADDING AMMONIUM SULFATE, SEPARATING THE FRACTION BETWEEN 0.3 AND 3.8 SATURATION, DIALYZING IN AQUEOUS SOLUTION, AND ADDING ACETONE TO THE SOLUTION TO SEPARATE THE ENZYME IN THE FORM OF CRYSTALS.

Claim 4

THE METHOD OF OBTAINING A CRYSTALLINE PROTEASE FROM A MOLD OF THE BLACK ASPERGILLUS TYPE SELECTED FROM THE GROUP CONSISTING OF ASPERGILLUS SAITOI, ASPERGILLUS USAMI, AND ASPERGILLUS AWAMORI, WHICH COMPRISES THE STEPS OD INOCULATING A NUTRIENT MEDIUM WITH THE ASPERGILLUS INCUBATING AT ORDINARY TEMPERATURE, EXTRACTING WITH WATER AT A PH OF ABOUT 2.5 TO 3.0, ADDING ETHYL ALCOHOL AND STARCH TO THE EXTRACT, REMOVING EXCESS STARCH, PRECIPITATING THE EXTRACT WITH ADDITIONAL ETHYL ALCOHOL, DISSOLVING THE PRECIPITATE IN AN ACETATE TYPE BUFFER AT PH OF ABOUT 3.0 ADDING CALCIUM ACETATE TO REMOVE IMPURITIER, DIALYZING THE SOLUTION TO REMOVE IMPURITIES, ADDING AMMONIUM SULFATE, SEPARATING THE FRACTION BETWEEN 0.3 AND 3.8 SATURATION, DIALYZING IN AQUEOUS SOLUTION, AND ADDING ACETONE TO THE SOLUTION TO SEPARATE THE ENZYME IN THE FORM OF CRYSTALS.