US3360441A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Microbiological screening process for aflatoxin
Assignee: Individual
•
Filing: 1965-09-16
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Granted: 1967-12-26
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Expires: 1985-09-16
Abstract
No abstract record available.
Inventors
- 👤 Harland R Burmeister
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 08:04
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/02; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/832; Y10S435/833; Y10S435/834; Y10S435/837
Claims Analysis
Total Claims: 2
Claim 1
Independent Claim
138 words
A MICROBIOLOGICAL PROCESS FOR DETECTING THE PRESENCE OF VERY LOW LEVELS OF AFLATOXIN CONTAMINATION IN SUSPECTED CEREAL GRAINS AND ANIMAL FEEDS COMPRISING EXTRACTING A PREDETERMINED QUANTITY OF THE SUSPECTED MATERIAL WITH CHLOROFORM TO OBTAIN ANY AFLATOXIN PRESENT THEREIN, PRECIPITATING THE CRUDE AFLATIOXIN THEREFROM WITH HEXANE, EVAPORATING THE HEXANE, SUSPENDING THE CRUDE AFLATOXIN IN STERILE WATER, FORMING A SERIAL DILUTION SERIES THEREOF, ADDING THE SAID SERIES TO RESPECTIVE CULTURE TUBES CONTAINING A TRYPTONE-GLUCOSE-YEAST EXTRACT AGAR MEDIUM ADJUSTED TO PH 6.2 AND HAVING DISPERSED THEREIN 1 PERCENT BY WEIGHT OF A SUSPENSION CONTAINING 3X10**9 SPORES PER MILLILITER OF AN AFLATIOXIN SENSITIVE MICROOGANISM SELECTED FROM THE GROUP CONSISTING OF BACILLUS MEGATERIUM NRRL B-1368, B. MEGATERIUM NRRL B-1370, AND B BREVIS NRRL B-1874, INCUBATING THE TUBES FOR 72 HOURS AT 30*C., AND INSPECTING THE CULTURE SURFACE FOR EVIDENCE OF BACTERIAL GROWTH THEREON.
Claim 2
Dependent Claim
138 words
A MICROBIOLOGICAL PROCESS FOR DETECTING THE PRESENCE OF VERY LOW LEVELS OF AFLATOXIN CONTAMINATION IN SUSPECTED CEREAL GRAINS AND ANIMAL FEEDS COMPRISING EXTRACTING A PREDETERMINED QUANTITY OF THE SUSPECTED MATERIAL WITH CHLOROFORM TO OBTAIN ANY AFLATOXIN PRESENT THEREIN, PRECIPITATING THE CRUDE AFLATIOXIN THEREFROM WITH HEXANE, EVAPORATING THE HEXANE, SUSPENDING THE CRUDE AFLATOXIN IN STERILE WATER, FORMING A SERIAL DILUTION SERIES THEREOF, ADDING THE SAID SERIES TO RESPECTIVE CULTURE TUBES CONTAINING A TRYPTONE-GLUCOSE-YEAST EXTRACT AGAR MEDIUM ADJUSTED TO PH 6.2 AND HAVING DISPERSED THEREIN 1 PERCENT BY WEIGHT OF A SUSPENSION CONTAINING 3X10**9 SPORES PER MILLILITER OF AN AFLATIOXIN SENSITIVE MICROOGANISM SELECTED FROM THE GROUP CONSISTING OF BACILLUS MEGATERIUM NRRL B-1368, B. MEGATERIUM NRRL B-1370, AND B BREVIS NRRL B-1874, INCUBATING THE TUBES FOR 72 HOURS AT 30*C., AND INSPECTING THE CULTURE SURFACE FOR EVIDENCE OF BACTERIAL GROWTH THEREON.
References Cited (Backward Citations)
US3126325A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1970-12-25 | 1971-06-26 | 1971-12-26 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1974-12-25 | 1975-06-26 | 1975-12-26 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1978-12-25 | 1979-06-26 | 1979-12-26 | $7700.00 | Window Closed / Lapsed |