US3723250A
Expired / Lapsed
US
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Proteolytic enzymes, their production and use
Assignee: Novo Terapeutisk Laboratorium AS
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Filing: 1968-09-23
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Granted: 1973-03-27
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Expires: 1988-09-23
Abstract
Abstract The invention relates to enzyme preparations containing novel proteolytic enzymes produced by cultivation of novel species of the genus Bacillus and showing useful activity at high alkalinities. The invention also relates to production of the novel proteolytic enzymes by cultivation of the novel species of the genus Bacillus in a nutrient medium having a pH-value within the range of 7 to 12. Finally the invention relates to a process of isolating the novel species referred to by effecting the isolation on nutrient media having a pH-value within the range of 9 to 11. The novel enzymes are particularly useful in detergent and dehairing compositions.
Inventors
- 👤 Knud Aunstrup
- 👤 Otto Andresen
- 👤 Helle Outtrup
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 15:51
CPC Classifications
A; A61; A61Q; A61Q9/00; A61Q9/04; A61K; A61K8/00; A61K8/18; A61K8/30; A61K8/64; A61K8/66; C; C11; C11D; C11D3/00; C11D3/16; C11D3/38; C11D3/386; C12; C12N; C12N9/00; C12N9/14; C12N9/48; C12N9/50; C12N9/52; C12N9/54; C14; C14C; C14C1/00; C14C1/06; C14C1/065; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/832
Claims Analysis
Total Claims: 3
Claim 1
Independent Claim
42 words
An enzyme preparation according to claim 1 wherein the proteolytic enzymes is derived from a bacterium selected from the group consisting of Bacillus species NCIB Nos. 10144 through 10148, 10281, 10284, 10286, 10288, 10301, 10304, 10306 through 10313 and 10315 through 10327.
Claim 2
Dependent Claim
114 words
A process for producing a proteolytic enzyme of the serine type showing optimal proteolytic activity against hemoglobin at a pH value above 9 and showing 80 to 100 percent of maximum proteolytic activity at pH 12 when said activities are measured by the Anson method the said method comprising aerobic cultivation of a member of the genus Bacillus selected from the group NCIB Nos. 10144 through 10148, 10281, 10284, 10286, 10288, 10301, 10304, 10306 through 30313 and 10315 through 10327 in a nutrient medium for production of proteolytic enzymes comprising assimilable carbon and nitrogen sources and maintaining the pH value of said nutrient medium between 7.5 and 10.5 during the main part of cultivation.
Claim 3
Dependent Claim
24 words
The method of hydrolyzing protein material which comprises contacting the protein material with an aqueous medium containing an enzyme preparation according to claim 1.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1976-03-26 | 1976-09-25 | 1977-03-27 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1980-03-26 | 1980-09-25 | 1981-03-27 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1984-03-26 | 1984-09-25 | 1985-03-27 | $7700.00 | Window Closed / Lapsed |