US3736042A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Microscope slide assembly
Assignee: Clinical Sciences Inc
•
Filing: 1971-05-05
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Granted: 1973-05-29
•
Expires: 1991-05-05
Abstract
Abstract A microscope slide assembly and a method of preparing such which includes an optically clear glass slide having a controlled thickness fluorocarbon layer coated on one planar surface. The layer is provided with a multiplicity of openings to form wells on the glass slide. An agar solution may be incorporated within the wells to provide an adhesive layer. In addition, a particular antigen may be layered on top of the agar composition to provide a completed assembly wherein only a patient''s serum is needed to process a microscopic examination. For use in fluoroescent microscopy, a film body grade Teflon containing an adhesive is used to provide the uniform fluorocarbon layer. The microscopic assembly may be enclosed within an environment isolation container to prevent contamination of the assembly from the ambient surroundings when the antigen is combined with the agar solution.
Inventors
- 👤 A L Markovits
- 👤 S W Wolfson
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 12:01
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54366; G01N1/00; G01N1/28; G02; G02B; G02B21/00; G02B21/34
Claims Analysis
Total Claims: 29
Claim 1
Independent Claim
41 words
The microscope slide system as recited in claim 1 wherein said plurality of openings include a predetermined area bounded by said fluorocarbon layer in a continuous manner, each of said plurality of openings being non-contiguous with respect to an adjacent opening.
Claim 2
Dependent Claim
23 words
The microscope slide system as recited in claim 1 wherein said fluorocarbon layer thickness is substantially equal to 1 X 10 4 inches.
Claim 3
Dependent Claim
34 words
The microscope slide system as recited in claim 1 wherein said fluorocarbon layer comprises a film bonding grade fluorocarbon having an adhesive for securing said layer to said boundary surface of said glass slide.
Claim 4
Dependent Claim
34 words
The microscope slide system as recited in claim 1 wherein said fluorocarbon layer includes a uniform predetermined thickness contiguous to said slide surface, said fluorocarbon layer being substantially composed of a tetrafluorethylene fluorocarbon resin.
Claim 5
Dependent Claim
21 words
The microscope slide system as recited in claim 5 wherein said thickness is substantially equal to 1 X 10 4 inches.
Claim 6
Dependent Claim
33 words
The microscope slide system as recited in claim 1 wherein said plurality of openings define a plurality of areas on said surface of said slide, said areas having an adhesive layer deposited thereon.
Claim 7
Dependent Claim
17 words
The microscope slide system as recited in claim 7 wherein said adhesive layer is composed of agar.
Claim 8
Dependent Claim
22 words
The microscope slide system as recited in claim 7 wherein said adhesive layer is composed of a .01% to .05% agarose solution.
Claim 9
Dependent Claim
21 words
The microscope slide system as recited in claim 9 wherein said adhesive layer is substantially composed of a .02% agarose solution.
Claim 10
Dependent Claim
30 words
The microscope slide system as recited in claim 10 wherein each of said plurality of areas includes a predetermined antigen layer deposited on an upper surface of said adhesive layer.
Claim 11
Dependent Claim
130 words
A fluorescent microscopy assembly, comprising: a. a microscope slide having a planar contour dimension with opposing boundary surfaces, one of said boundary surfaces having a fluorocarbon layer fixedly deposited thereon, said layer having a predetermined thickness within an approximating range between 7.5 X 10 5 and 1.5 X 10 4 inches, said fluorocarbon layer further having a plurality of openings passing therethrough for forming reactant regions contiguous to said boundary surface of said slide; and, b. enclosing means for said microscope slide, said enclOsing means having an external surface substantially impervious to moisture for isolating said slide from an external environment, said enclosing means comprising a pair of adjacently positioned elements fastened each to the other on a common boundary periphery to form a total enclosure for said microscope slide.
Claim 12
Dependent Claim
36 words
The fluorescent microscopy assembly as recited in claim 12 wherein said plurality of openings of said fluorocarbon layer define a multiplicity of apertures passing through said fluorocarbon layer to said boundary surface contiguous with said layer.
Claim 13
Dependent Claim
29 words
The fluorescent microscopy assembly as recited in claim 13 wherein each of said multiplicity of apertures defines a reactant region bounded by said fluorocarbon layer in a continuous manner.
Claim 14
Dependent Claim
24 words
The fluoroescent microscopy assembly as recited in claim 14 wherein each of said multiplicity of apertures is non-contiguous with respect to an adjacent aperture.
Claim 15
Dependent Claim
23 words
The fluoroescent microscopy assembly as recited in claim 12 wherein said fluorocarbon layer thickness is substantially equal to 1 X 10 4 inches.
Claim 16
Dependent Claim
42 words
The fluoroescent microscopy assembly as recited in claim 12 wherein said plurality of openings define a plurality of areas on said surface of said slide, said areas having an adhesive layer deposited thereon, said deposited ashesive layer defining an upper surface plane.
Claim 17
Dependent Claim
17 words
The fluorescent microscopy assembly as recited in claim 17 wherein said adhesive layer is composed of agar.
Claim 18
Dependent Claim
22 words
The fluorescent microscopy assembly as recited in claim 17 wherein said adhesive layer is composed of a .01% to .05% agarose solution.
Claim 19
Dependent Claim
21 words
The fluorescent microscopy assembly as recited in claim 17 wherein said adhesive layer is substantially composed of a .02% agarose solution.
Claim 20
Dependent Claim
31 words
The fluorescent microscopy assembly as recited in claim 17 wherein each of said plurality of areas includes a predetermined antigen layer deposited on said upper surface plane of said adhesive layer.
Claim 21
Dependent Claim
22 words
The fluorescent microscopy assembly as recited in claim 21 wherein said deposited antigen and adhesive layers are confined to said reactant regions.
Claim 22
Dependent Claim
21 words
The fluorescent microscopy assembly as recited in claim 22 wherein said adhesive layer is substantially composed of a .02% agarose solution.
Claim 23
Dependent Claim
27 words
The fluorescent microscopy assembly as recited in claim 23 wherein said .02% agarose solution layer and said predetermined antigen layer are deposited only within said reactant regions.
Claim 24
Dependent Claim
21 words
The fluorescent microscopy assembly as recited in claim 12 wherein said fluorocarbon layer is substantially composed of a tetrafluorethylene fluorocarbon resin.
Claim 25
Dependent Claim
23 words
The fluorescent microscopy assembly as recited in claim 25 wherein said layer has a thickness substantially equal to 1 X 10 4 inches.
Claim 26
Dependent Claim
23 words
The fluorescent microscopy assembly as recited in claim 12 wherein said external surface of said enclosing means is formed of an aluminum foil.
Claim 27
Dependent Claim
24 words
The fluorescent microscopy assembly as recited in claim 12 wherein said external surface of said enclosing means is formed of moisture impervious plastic sheeting.
Claim 28
Dependent Claim
32 words
The fluorescent microscopy assembly as recited in claim 12 wherein said enclosing means defines an inner volume having an inert gas for presenting an internal inert environment to said enclosed microscope slide.
Claim 29
Dependent Claim
31 words
The fluorescent microscopy assembly as recited in claim 12 wherein said enclosing means includes a desiccant for insertion adjacent said slide for preserving a moisture free environment within said enclosing means.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1976-05-28 | 1976-11-27 | 1977-05-29 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1980-05-28 | 1980-11-27 | 1981-05-29 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1984-05-28 | 1984-11-27 | 1985-05-29 | $7700.00 | Window Closed / Lapsed |