US3843777A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Hemolytic assay system
Assignee: Baxter International Inc
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Filing: 1972-08-10
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Granted: 1974-10-22
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Expires: 1992-08-10
Abstract
Abstract A rapid and convenient method for the detection and quantitation of complement fixing antibodies in blood serum consisting of admixing predetermined amounts of complement and complement fixing antigen with said serum and then reacting the mixture with a known amount of hemolysin-sensitized erythrocytes in a semisolid gel medium for a predetermined period of time.
Inventors
- 👤 M Hainski
- 👤 G Ordonez
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 10:29
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/558; G01N33/559
Claims Analysis
Total Claims: 2
Claim 1
Independent Claim
179 words
A DIAGNOSTIC METHOD FOR THE DETERMINATION OF THE PRESENCE OF ANTIBODIES TO KOLMER ANTIGEN IN BLOOD SERUM COMPRISING ADMIXING (A) ABOUT ONE PART OF VOLUME OF GUNIEA PIG COMPLEMENT RECONSISTUTED IN KOLMER SALINE WITH (B) ABOUT ONE PART BY VOLUME OF KOLMER ANTIGEN AND (C) ABOUT ONE PART BY VOLUME OF BLOOD SERUM AND THEN (D) REACTING THE RESULTING MIXTURE FOR A PREDETERMINED PERIOD OF TIME WITH HEMOLYSINSENSITIZED ERYTHROCYTES HOMOGENEOUSLY DISPERSED IN A SEMISOLID GEL MEDIUM CONTAINING FROM ABOUT 0.1 TO ABOUT 5 PERCENT BY WEIGHT AGAR ON A PLATE BY ALLOWING SAID MIXTURE TO COME INTO CONTACT WITH SAID HEMOLYSIN-SENSITIZED ERYTHOCYTES BY RADIAL DIFFUSION FROM A WELL IN THE SURFACE OF SAID GEL DURING INCUBATION AT ABOUT 37*C FOR A PREDETERMINED PERIOD OF TIME, SAID HEMOLYSIN-SENSITIZED ERTHROCYTES COMPRISING (E) ABOUT ONE PART BY VOLUME SHEEP CELL HEMOLYSIN IN ABUT 10 TO 100 PARTS BY VOLUME KOLMER SALIME ADMIXING WITH (F) ABOUT ONE PART BY VOLUME SHEEP ERYTHCROYTES DISPERSED AT A CONCENTRATION OF ABOUT 1 TO 5 PERCENT KOLMER SALINE, SAID KOLMER SALINE COMPRISING AQUEOUS NACL, MGCL2.6H2O AND CACL2.2H2O.
Claim 2
Dependent Claim
179 words
A DIAGNOSTIC METHOD FOR THE DETERMINATION OF THE PRESENCE OF ANTIBODIES TO KOLMER ANTIGEN IN BLOOD SERUM COMPRISING ADMIXING (A) ABOUT ONE PART OF VOLUME OF GUNIEA PIG COMPLEMENT RECONSISTUTED IN KOLMER SALINE WITH (B) ABOUT ONE PART BY VOLUME OF KOLMER ANTIGEN AND (C) ABOUT ONE PART BY VOLUME OF BLOOD SERUM AND THEN (D) REACTING THE RESULTING MIXTURE FOR A PREDETERMINED PERIOD OF TIME WITH HEMOLYSINSENSITIZED ERYTHROCYTES HOMOGENEOUSLY DISPERSED IN A SEMISOLID GEL MEDIUM CONTAINING FROM ABOUT 0.1 TO ABOUT 5 PERCENT BY WEIGHT AGAR ON A PLATE BY ALLOWING SAID MIXTURE TO COME INTO CONTACT WITH SAID HEMOLYSIN-SENSITIZED ERYTHOCYTES BY RADIAL DIFFUSION FROM A WELL IN THE SURFACE OF SAID GEL DURING INCUBATION AT ABOUT 37*C FOR A PREDETERMINED PERIOD OF TIME, SAID HEMOLYSIN-SENSITIZED ERTHROCYTES COMPRISING (E) ABOUT ONE PART BY VOLUME SHEEP CELL HEMOLYSIN IN ABUT 10 TO 100 PARTS BY VOLUME KOLMER SALIME ADMIXING WITH (F) ABOUT ONE PART BY VOLUME SHEEP ERYTHCROYTES DISPERSED AT A CONCENTRATION OF ABOUT 1 TO 5 PERCENT KOLMER SALINE, SAID KOLMER SALINE COMPRISING AQUEOUS NACL, MGCL2.6H2O AND CACL2.2H2O.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1977-10-21 | 1978-04-22 | 1978-10-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1981-10-21 | 1982-04-22 | 1982-10-22 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1985-10-21 | 1986-04-22 | 1986-10-22 | $7700.00 | Window Closed / Lapsed |