Abstract 1. A PROCESS FOR THE PREPARATION OF A BIFUNCTIONALLY CROSSLINKED INSULIN DERIVATIVE IN WHICH THE AMINO GROUP OF THE A-1 GLYCINE IS LINKED TO THE $AMINO GROUP OF THE B-29 LYSINE BY A BRIDGE OF THE FORMULA: -CO-X-COIN WHICH X IS A CARBON-CARBON-CARBON BOND, ALKYLENE OF 1 TO 15 CARBON ATOMS, OR ALKYLENE OF 1 TO 15 CARBON ATOMS WHEREIN 1 CARBON ATOM IS REPLACED BY SULPHUR, OXYGEN OR IMINO, SAID ALKYLENE BEING UNSUBSTITUTED OR SUBSTITUTED BY ONE OR TWO HYDROPHILLIC GROUPS WHICH COMPRISES THE STEPS OF REACTING THE INSULIN A-CHAIN IN WHICH THE FOUR FREE MERCAPTO GROUPS HAVE BEEN PROTECTED, WITH AN ACTIVATED DERIVATIVE OF A DISCARBOXYLIC ACID OF THE FORMULA: Z-CO-X-CO-Z IN WHICH X IS AS ABOVE DEFINED AND Z IS A SUITABLE ACID ACTIVATING GROUP, TO PRODUCE AN INSULIN A-CHAIN DERIVATIVE MONOFUNCTIONALLY SUBSTITUTED ON THE AMINO GROUP OF TIVATED DERIVATIVE OF A DICARBOXYLIC ACID OF THE FORMULA: B-CHAIN IN WHICH THE TWO FREE MERCAPTO GROUPS HAVE BEEN PROTECTED, TO YIELD A COMPOUND OF THE FORMULA: ASN(A21)-(CYS(-S-Y))4-GLY(A1)-NH-CO-X-CO-NHLYS(B29)(-ALA)-CYS(-S-Y)-CYS(-S -Y)-PHE(B1)-NH2 IN WHICH X IS AS ABOVE DEFINED AND Y IS THE MERCAPTO PROTECTING GROUP, REMOVING THE MERCAPTO GROUPS, AND OXIDIZING THE MERCAPTO GROUPS IN AN AQUEOUS ALKALINE MEDIUM TO FORM DISULFIDE BONDS.
A PROCESS FOR THE PREPARATION OF A BIFUNCTIONALLY CROSSLINKED INSULIN DERIVATIVE IN WHICH THE AMINO GROUP OF THE A-1 GLYCINE IS LINKED TO THE $AMINO GROUP OF THE B-29 LYSINE BY A BRIDGE OF THE FORMULA: -CO-X-COIN WHICH X IS A CARBON-CARBON-CARBON BOND, ALKYLENE OF 1 TO 15 CARBON ATOMS, OR ALKYLENE OF 1 TO 15 CARBON ATOMS WHEREIN 1 CARBON ATOM IS REPLACED BY SULPHUR, OXYGEN OR IMINO, SAID ALKYLENE BEING UNSUBSTITUTED OR SUBSTITUTED BY ONE OR TWO HYDROPHILLIC GROUPS WHICH COMPRISES THE STEPS OF REACTING THE INSULIN A-CHAIN IN WHICH THE FOUR FREE MERCAPTO GROUPS HAVE BEEN PROTECTED, WITH AN ACTIVATED DERIVATIVE OF A DISCARBOXYLIC ACID OF THE FORMULA: Z-CO-X-CO-Z IN WHICH X IS AS ABOVE DEFINED AND Z IS A SUITABLE ACID ACTIVATING GROUP, TO PRODUCE AN INSULIN A-CHAIN DERIVATIVE MONOFUNCTIONALLY SUBSTITUTED ON THE AMINO GROUP OF TIVATED DERIVATIVE OF A DICARBOXYLIC ACID OF THE FORMULA: B-CHAIN IN WHICH THE TWO FREE MERCAPTO GROUPS HAVE BEEN PROTECTED, TO YIELD A COMPOUND OF THE FORMULA: ASN(A21)-(CYS(-S-Y))4-GLY(A1)-NH-CO-X-CO-NHLYS(B29)(-ALA)-CYS(-S-Y)-CYS(-S -Y)-PHE(B1)-NH2 IN WHICH X IS AS ABOVE DEFINED AND Y IS THE MERCAPTO PROTECTING GROUP, REMOVING THE MERCAPTO GROUPS, AND OXIDIZING THE MERCAPTO GROUPS IN AN AQUEOUS ALKALINE MEDIUM TO FORM DISULFIDE BONDS.
Claim 2
Dependent Claim
201 words
A PROCESS FOR THE PREPARATION OF A BIFUNCTIONALLY CROSSLINKED INSULIN DERIVATIVE IN WHICH THE AMINO GROUP OF THE A-1 GLYCINE IS LINKED TO THE $AMINO GROUP OF THE B-29 LYSINE BY A BRIDGE OF THE FORMULA: -CO-X-COIN WHICH X IS A CARBON-CARBON-CARBON BOND, ALKYLENE OF 1 TO 15 CARBON ATOMS, OR ALKYLENE OF 1 TO 15 CARBON ATOMS WHEREIN 1 CARBON ATOM IS REPLACED BY SULPHUR, OXYGEN OR IMINO, SAID ALKYLENE BEING UNSUBSTITUTED OR SUBSTITUTED BY ONE OR TWO HYDROPHILLIC GROUPS WHICH COMPRISES THE STEPS OF REACTING THE INSULIN A-CHAIN IN WHICH THE FOUR FREE MERCAPTO GROUPS HAVE BEEN PROTECTED, WITH AN ACTIVATED DERIVATIVE OF A DISCARBOXYLIC ACID OF THE FORMULA: Z-CO-X-CO-Z IN WHICH X IS AS ABOVE DEFINED AND Z IS A SUITABLE ACID ACTIVATING GROUP, TO PRODUCE AN INSULIN A-CHAIN DERIVATIVE MONOFUNCTIONALLY SUBSTITUTED ON THE AMINO GROUP OF TIVATED DERIVATIVE OF A DICARBOXYLIC ACID OF THE FORMULA: B-CHAIN IN WHICH THE TWO FREE MERCAPTO GROUPS HAVE BEEN PROTECTED, TO YIELD A COMPOUND OF THE FORMULA: ASN(A21)-(CYS(-S-Y))4-GLY(A1)-NH-CO-X-CO-NHLYS(B29)(-ALA)-CYS(-S-Y)-CYS(-S -Y)-PHE(B1)-NH2 IN WHICH X IS AS ABOVE DEFINED AND Y IS THE MERCAPTO PROTECTING GROUP, REMOVING THE MERCAPTO GROUPS, AND OXIDIZING THE MERCAPTO GROUPS IN AN AQUEOUS ALKALINE MEDIUM TO FORM DISULFIDE BONDS.