US3853709A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for preparing nebramycin factors ii and vii
Assignee: Eli Lilly and Co
•
Filing: 1972-12-08
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Granted: 1974-12-10
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Expires: 1992-12-08
Abstract
Abstract A process for producing antibiotic nebramycin II, a component antibiotic of the multifactored antibiotic complex nebramycin, and nebramycin VII by culturing Streptomyces tenebrarius NRRL 3816, mutant strain ex Streptomyces tenebrarius ATCC 17920 in an aqueous nutrient culture medium under aerobic fermentation conditions. Nebramycin II is recovered from the filtered fermentation broth by chromatography over a cationic exchange resin and is separated from the newly discovered co-produced minor factor, nebramycin VII, by further chromatography over a cationic exchange resin.
Inventors
- 👤 W Stark
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 20:26
CPC Classifications
C; C07; C07H; C07H15/00; C07H15/20; C07H15/22; C07H15/222; C07H15/224; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/886
Claims Analysis
Total Claims: 4
Claim 1
Independent Claim
59 words
THE PROCESS FOR PRODUCING NEBRAMYCIN II AND NEBRAMYCIN VII WHICH COMPRISES CULTIVATING STTEPTOMYCES TENEBRARIUS NRRL 3816, MUTANT STRAIN EX STTEPTOMYCES TENEBRARIUS ATCC 17920 IN AN AQUEOUS NUTRIENT CULTURE MEDIUM CONTAINING ASSIMILABLE SOURCES OF CARBON, NITROGEN AND INORGANIC SALTS UNDER SUBMERGED AEROBIC FERMENTATION CONDITIONS UNTIL A SUBSTANTIAL AMOUNT OF ANTIBIOTIC ACTIVITY IS PRODUCED BY SAID ORGANISM IN SAID CULTURE MEDIUM.
Claim 2
Dependent Claim
59 words
THE PROCESS FOR PRODUCING NEBRAMYCIN II AND NEBRAMYCIN VII WHICH COMPRISES CULTIVATING STTEPTOMYCES TENEBRARIUS NRRL 3816, MUTANT STRAIN EX STTEPTOMYCES TENEBRARIUS ATCC 17920 IN AN AQUEOUS NUTRIENT CULTURE MEDIUM CONTAINING ASSIMILABLE SOURCES OF CARBON, NITROGEN AND INORGANIC SALTS UNDER SUBMERGED AEROBIC FERMENTATION CONDITIONS UNTIL A SUBSTANTIAL AMOUNT OF ANTIBIOTIC ACTIVITY IS PRODUCED BY SAID ORGANISM IN SAID CULTURE MEDIUM.
Claim 3
Dependent Claim
34 words
The process of claim 1 wherein the culture medium is maintained at a temperature between about 37* and 43*C. and the growth of the organism is carried out for about 4 to 7 days.
Claim 4
Dependent Claim
20 words
The process of claim 1 wherein the growth of the organism is carried out in the absence of visible light.
References Cited (Backward Citations)
US3691279A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1977-12-09 | 1978-06-10 | 1978-12-10 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1981-12-09 | 1982-06-10 | 1982-12-10 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1985-12-09 | 1986-06-10 | 1986-12-10 | $7700.00 | Window Closed / Lapsed |