Patent US2325955A successfully refreshed from Google Patents.
US3857966A
Expired / Lapsed
US
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Process for bland, soluble protein
Assignee: General Foods Corp
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Filing: 1973-08-16
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Granted: 1974-12-31
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Expires: 1993-08-16
Abstract
Abstract An improved process produces protein hydrolysates in greater yields and having better taste than has heretofore been possible. According to a preferred embodiment of this process, a protein is initially heat treated at alkaline pH to condition the protein. The protein is then reacted first at alkaline pH with an alkaline microbial protease and then at neutral pH with a plant enzyme and a neutral microbial protease. The resultant hydrolysate can be winterized, clarified, flash concentrated and dried to produce a bland, clear, acid and heat stable protein supplement for foods.
Inventors
- 👤 C Wiener
- 👤 J Lugay
- 👤 J Feldman
- 👤 G Haas
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 12:34
CPC Classifications
A; A23; A23J; A23J3/00; A23J3/04; A23J3/06; A23J3/30; A23J3/32; A23J3/34
Claims Analysis
Total Claims: 25
Claim 1
Independent Claim
44 words
AN IMPROVED PROCESS FOR PREPARING A BLAND, SOLUBLE NUTRITIOUS ENZYMATICALLY HYDROLYZED PROTEINACEOUS MATERIAL COMPRISING: A. FIRST REACTING A PROTEIN AT ALKALINE PH WITH AN ALKALINE MICROBIAL PROTEASE; B. THEN REATING THE PROTEIN AT NEUTRAL PH WITH A PLANT ENZYME AND A NEUTRAL MICROBIAL PROTEASE.
Claim 2
Dependent Claim
44 words
AN IMPROVED PROCESS FOR PREPARING A BLAND, SOLUBLE NUTRITIOUS ENZYMATICALLY HYDROLYZED PROTEINACEOUS MATERIAL COMPRISING: A. FIRST REACTING A PROTEIN AT ALKALINE PH WITH AN ALKALINE MICROBIAL PROTEASE; B. THEN REATING THE PROTEIN AT NEUTRAL PH WITH A PLANT ENZYME AND A NEUTRAL MICROBIAL PROTEASE.
Claim 3
Dependent Claim
18 words
An improved process according to claim 1 wherein the alkaline microbial protease is one derived from B. Subtilis.
Claim 4
Dependent Claim
13 words
An improved process according to claim 1 wherein the plant enzyme is papain.
Claim 5
Dependent Claim
18 words
An improved process according to claim 1 wherein the neutral microbial protease is one derived from B. Subtilis.
Claim 6
Dependent Claim
34 words
An improved process according to claim 1 wherein the alkaline microbial protease is one derived from B. Subtilis, the plant enzyme is papain, and the neutral microbial protease is one derived from B. Subtilis.
Claim 7
Dependent Claim
101 words
An improved process for preparing a bland, soluble, nutritious, enzymatically hydrolyzed proteinaceous material comprising: a. suspending a protein in water; b. establishing the pH of the resulting suspension at within the alkaline range and adusting the temperature of the suspension to within the range of from about 25* to 65*C; c. adding alkaline microbial protease and continuing the reaction until the pH drops to within the range of about 6.5 to 7.5; and d. adding to the suspension a plant enzyme and neutral microbial protease and continuing the reaction for a period of time effective to produce a bland, soluble hydrolysate.
Claim 8
Dependent Claim
101 words
An improved process for preparing a bland, soluble, nutritious, enzymatically hydrolyzed proteinaceous material comprising: a. suspending a protein in water; b. establishing the pH of the resulting suspension at within the alkaline range and adusting the temperature of the suspension to within the range of from about 25* to 65*C; c. adding alkaline microbial protease and continuing the reaction until the pH drops to within the range of about 6.5 to 7.5; and d. adding to the suspension a plant enzyme and neutral microbial protease and continuing the reaction for a period of time effective to produce a bland, soluble hydrolysate.
Claim 9
Dependent Claim
18 words
An improved process according to claim 6 wherein the alkaline microbial protease is one derived from B. Subtilis.
Claim 10
Dependent Claim
13 words
An improved process according to claim 6 wherein the plant enzyme is papain.
Claim 11
Dependent Claim
18 words
An improved process according to claim 6 wherein the neutral microbial protease is one derived from B. Subtilis.
Claim 12
Dependent Claim
34 words
An improved process according to claim 6 wherein the alkaline microbial protease is one derived from B. Subtilis, the plant enzyme is papain, and the neutral microbial protease is one derived from B. Subtilis.
Claim 13
Dependent Claim
42 words
An improved process according to claim 6 including the further step of heating the suspension of protein in water, before addition of the enzymes, at an alkaline pH at a temperature and for a time effective to condition the protein for hydrolysis.
Claim 14
Dependent Claim
30 words
An improved process according to claim 6 which further includes the step of heating the suspension, after hydrolysis, to a temperature and for a time sufficient to inactivate the enzymes.
Claim 15
Dependent Claim
26 words
An improved method according to claim 6 which further includes the step of separating the resulting hydrolysate solution from the insoluble material remaining in the suspension.
Claim 16
Dependent Claim
48 words
An improved process according to claim 13 which further includes: a. ajusting the pH of the hydrolysate solution to about 4.0; b. chilling the solutioN to a temperature of about 0*C for from about 6 to 24 hours; and c. separating the insoluble portion from the soluble portion.
Claim 17
Dependent Claim
48 words
An improved process according to claim 14 which further includes: a. adding about 60 g of diatomaceous earth for each liter of the hydrolysate solution before cooling; and b. filtering the solution through a filter coated with about 20 g of diatomaceous earth per liter of hydrolysate solution.
Claim 18
Dependent Claim
22 words
An improved process according to claim 6 which further includes flash concentrating and drying the hydrolysate solution to obtain a dry hydrolysate.
Claim 19
Dependent Claim
13 words
An improved process according to claim 6 wherein the protein comprises soy protein.
Claim 20
Dependent Claim
14 words
An improved process according to claim 6 wherein the protein comprises egg albumin protein.
Claim 21
Dependent Claim
13 words
An improved process according to claim 6 wherein the protein comprises fish protein.
Claim 22
Dependent Claim
13 words
An improved process according to claim 6 wherein the protein comprises whey protein.
Claim 23
Dependent Claim
16 words
An improved process according to claim 6 wherein the reaction temperature is maintained at about 50*C.
Claim 24
Dependent Claim
34 words
An improved process according to claim 6 wherein the reaction temperature is maintained at about 50*C and the reaction is continued for about 100 to about 110 minutes after addition of the first enzyme.
Claim 25
Dependent Claim
29 words
An improved process according to claim 6 wherein the pH of the suspension of step (b) is adjusted to within the range of from about 8.0 to about 8.5.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1977-12-30 | 1978-07-01 | 1978-12-31 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1981-12-30 | 1982-07-01 | 1982-12-31 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1985-12-30 | 1986-07-01 | 1986-12-31 | $7700.00 | Window Closed / Lapsed |