US3869365A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method in counter flow isotachophoresis
Assignee: Pfizer Health AB
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Filing: 1973-12-12
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Granted: 1975-03-04
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Expires: 1993-12-12
Abstract
Abstract A method for fixing the sample in a certain position in a counter flow isotachophoresis column comprises the adjustment of voltage and counterflow values to a constant at which the power from the electric field and the power from the counterflow compensate each other at the desired position of the sample.
Inventors
- 👤 Bengt Fritiof Sunden
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 13:20
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447; G01N27/44756; G01N27/44765
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
180 words
METHOD IN COUNTER FLOW ISOTACHOPHORESIS OF THE TYPE WHEREIN A SAMPLE COMPRISING IONS OF THE SAME POLARITY TO BE SEPARATED IS INTRODUCED INTO A COLUMN PROVIDED WITH FIRST AND SECOND ELECTRODES, WITH A FIRST ELECTROLYTE BETWEEN THE SAMPLE AND SAID FIRST ELECTRODE AND A SECOND ELECTROLYTE BETWEEN THE SAMPLE ANS SAID SECOND ELECTRODE, THE FIRST AND SECOND ELECTROTYLES COMPRISING IONS OF HIGHER AND LOWER MOBILITIES RESPECTIVELY THAN THE IONS OF THE SAMPLE AND AN ELECTRICAL POTENTIAL IS APPLIED BETWEEN SAID FIRST AND SECOND ELECTRODES HAVING A POLARITY SUCH THAT THE SAMPLE IONS WILL TEND TO MIGRATE TOWARDS SAID FIRST ELECTRODE AND A PRESSURE DIFFERENCE IS APPLIED BETWEEN SAID FIRST AND SECOND ELECTROLYTES, THE IMPROVEMENT WHICH INCLUDES THE STEPS OF: INDEPENDENTLY ADJUSTING THE VALUE OF SAID ELECTRICAL POTENTIAL AND THE VALUE OF PRESSURE DIFFERENCE TO PRODUCE WITH THE AID OF A CURRENT DIFFERENTIAL DETECTOR AN ELECTRICAL CURRENT FLOW HAVING A CONSTANT VALUE SUCH THAT THE INFLUENCE OF SAID CONSTANT CURRENT FLOW EQUALIZES TO THE ADJUSTED OPPOSING COUNTER FLOW OF ELECTROLYTE TO MAINTAIN THE SAMPLE AT A DESIRED POSITION IN THE COLUMN.
Claim 2
Dependent Claim
180 words
METHOD IN COUNTER FLOW ISOTACHOPHORESIS OF THE TYPE WHEREIN A SAMPLE COMPRISING IONS OF THE SAME POLARITY TO BE SEPARATED IS INTRODUCED INTO A COLUMN PROVIDED WITH FIRST AND SECOND ELECTRODES, WITH A FIRST ELECTROLYTE BETWEEN THE SAMPLE AND SAID FIRST ELECTRODE AND A SECOND ELECTROLYTE BETWEEN THE SAMPLE ANS SAID SECOND ELECTRODE, THE FIRST AND SECOND ELECTROTYLES COMPRISING IONS OF HIGHER AND LOWER MOBILITIES RESPECTIVELY THAN THE IONS OF THE SAMPLE AND AN ELECTRICAL POTENTIAL IS APPLIED BETWEEN SAID FIRST AND SECOND ELECTRODES HAVING A POLARITY SUCH THAT THE SAMPLE IONS WILL TEND TO MIGRATE TOWARDS SAID FIRST ELECTRODE AND A PRESSURE DIFFERENCE IS APPLIED BETWEEN SAID FIRST AND SECOND ELECTROLYTES, THE IMPROVEMENT WHICH INCLUDES THE STEPS OF: INDEPENDENTLY ADJUSTING THE VALUE OF SAID ELECTRICAL POTENTIAL AND THE VALUE OF PRESSURE DIFFERENCE TO PRODUCE WITH THE AID OF A CURRENT DIFFERENTIAL DETECTOR AN ELECTRICAL CURRENT FLOW HAVING A CONSTANT VALUE SUCH THAT THE INFLUENCE OF SAID CONSTANT CURRENT FLOW EQUALIZES TO THE ADJUSTED OPPOSING COUNTER FLOW OF ELECTROLYTE TO MAINTAIN THE SAMPLE AT A DESIRED POSITION IN THE COLUMN.
Claim 3
Dependent Claim
36 words
Method according to claim 1, characterized in, that the adjustment of said values is made by using a current differerential detector, the values being chosen so as to make the derivative of the electrical current zero.
Claim 4
Dependent Claim
29 words
Method according to claim 1, characterized in, that the values of the counter flow and the voltage are adjusted by means of an indication means located along the column.
Claim 5
Dependent Claim
15 words
Method according to claim 3, characterized in, that the indication means consists of a thermodetector.
Claim 6
Dependent Claim
15 words
Method according to claim 3, characterized in, that the indication means consists of a UV-detector.
Claim 7
Dependent Claim
16 words
Method according to claim 3, characterized in, that the indication means consists of a conductivity detector.
Claim 8
Dependent Claim
16 words
Method according to claim 3, characterized in, that the indication means consists of a potential detector.
Claim 9
Dependent Claim
26 words
The method according to claim 1, which includes the step of introducing the sample into the column in the absence of a counter flow of electrolyte.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-03-03 | 1978-09-02 | 1979-03-04 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-03-03 | 1982-09-02 | 1983-03-04 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-03-03 | 1986-09-02 | 1987-03-04 | $7700.00 | Window Closed / Lapsed |