US3880715A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Immunoassay for haptens
Assignee: Syva Co
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Filing: 1973-06-13
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Granted: 1975-04-29
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Expires: 1993-06-13
Abstract
Abstract Haptens are determined by an immunoassay employing lysozyme and Micrococcus lysodeikticus cells wherein the response of bacterial cells to lysis by lysozyme is greatly stabilized by heating lyophilized dry bacterial cells at an elevated temperature for a time sufficient to improve the stability of the bacterial cells, when reconstituted as a suspension in an aqueous medium.
Inventors
- 👤 Richard S Schneider
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 22:25
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/536; G01N33/542; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/859; Y10S435/862; Y10S435/964
Claims Analysis
Total Claims: 3
Claim 1
Independent Claim
202 words
IN A METHOD FOR CARRYING OUT AN IMMUNOASSAY, WHEREIN A HAPTEN IS COVALENTLY BOUND TO LYSOZYME AND THE ACTIVITY OF THE LYSOZYME IS SUBSTANTIALLY REDUCED WHEN THE LYOZYME TO WHICH THE HAPTEN IS COVALENTLY BOUND IS BOUND TO AN ANTIBODY FOR SAID HAPTEN, AND THE CONTENT OF THE HAPTEN IN THE SOLUTION IS DETERMINED IN THE IMMUNOASSAY AS A RESULT OF FREE HAPTEN IN SOLUTION COMPETING WITH HAPTEN BOUND TO LYOZYME, WHICH WHEN BOUND TO AN ANTIBODY IN THE SOLUTION PRODUCES A DIFFERENT LYOZYME ACTIVITY, SO THAT THE AMOUNT OF HAPTEN BOUND TO LYSOZYME WHICH IS BOUND TO ANTIBODY IS LESS IN THE PRESENCE OF FREE HAPTEN AS COMPARED TO THE ABSENCE OF FREE HAPTEN, AND BY EMPLOYING STANDARDIZED SOLUTIONS CONTAINING KNOWN AMOUNTS OF HAPTEN AND RELATING THE LYOZYME ACTIVITY AGAINST M. LYSODEIKTICUS OVER A SPECIFIED PERIOD OF TIME TO THE CONCENTRATION OF HAPTEN PRESENT IN THE STANDARD SOLUTION, THE IMPROVEMENT WHICH COMPRISES EMPLOYING M. LYSODEIKTICUS IN SAID IMMUNOASSAY, AS A SUBSTRATE FOR SAID LYSOZYME, WHICH HAS BEEN HEATED IN A LYOPHILIZED DRY STATE AT A TEMPERATURE IN THE RANGE OF ABOUT 30*-100*C FOR A TIME IN THE RANGE OF ABOUT 1 HOUR TO 86 HOURS, THE TIME AND TEMPERATURE VARYING INVERSELY.
Claim 2
Dependent Claim
202 words
IN A METHOD FOR CARRYING OUT AN IMMUNOASSAY, WHEREIN A HAPTEN IS COVALENTLY BOUND TO LYSOZYME AND THE ACTIVITY OF THE LYSOZYME IS SUBSTANTIALLY REDUCED WHEN THE LYOZYME TO WHICH THE HAPTEN IS COVALENTLY BOUND IS BOUND TO AN ANTIBODY FOR SAID HAPTEN, AND THE CONTENT OF THE HAPTEN IN THE SOLUTION IS DETERMINED IN THE IMMUNOASSAY AS A RESULT OF FREE HAPTEN IN SOLUTION COMPETING WITH HAPTEN BOUND TO LYOZYME, WHICH WHEN BOUND TO AN ANTIBODY IN THE SOLUTION PRODUCES A DIFFERENT LYOZYME ACTIVITY, SO THAT THE AMOUNT OF HAPTEN BOUND TO LYSOZYME WHICH IS BOUND TO ANTIBODY IS LESS IN THE PRESENCE OF FREE HAPTEN AS COMPARED TO THE ABSENCE OF FREE HAPTEN, AND BY EMPLOYING STANDARDIZED SOLUTIONS CONTAINING KNOWN AMOUNTS OF HAPTEN AND RELATING THE LYOZYME ACTIVITY AGAINST M. LYSODEIKTICUS OVER A SPECIFIED PERIOD OF TIME TO THE CONCENTRATION OF HAPTEN PRESENT IN THE STANDARD SOLUTION, THE IMPROVEMENT WHICH COMPRISES EMPLOYING M. LYSODEIKTICUS IN SAID IMMUNOASSAY, AS A SUBSTRATE FOR SAID LYSOZYME, WHICH HAS BEEN HEATED IN A LYOPHILIZED DRY STATE AT A TEMPERATURE IN THE RANGE OF ABOUT 30*-100*C FOR A TIME IN THE RANGE OF ABOUT 1 HOUR TO 86 HOURS, THE TIME AND TEMPERATURE VARYING INVERSELY.
Claim 3
Dependent Claim
32 words
In a method according to claim 1, wherein said time is in the range of 6 hours to 72 hours, and said temperature is in the range of about 40* to 75*C.
References Cited (Backward Citations)
US3753858A
Cited
US3649454A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-04-28 | 1978-10-28 | 1979-04-29 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-04-28 | 1982-10-28 | 1983-04-29 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-04-28 | 1986-10-28 | 1987-04-29 | $7700.00 | Window Closed / Lapsed |