Gel chromatography material and preparation thereof

Abstract

Abstract A new gel chromatography material is prepared as uniform spherical particles by the suspension polymerization of N, N''(diacrylamidomethyl) ethylene or propylene urea either alone or together with one or more than one monomer having a ethylenically unsaturated double bond and copolymerizable therewith, such as acrylamide, methacrylamide, acrylic acid, methacrylic acid, itaconic acid, maleic acid and bis-acrylamide. The gel chromatography material has a structure characterized by a relatively large pore size due to the long chain of the urea compound acting as a cross-linking agent, and the pore size may be altered by changing the molar ratio of the urea compound to the copolymerizing monomers. The gel chromatography material is useful not only for separating various high molecular weight substances, such as proteins, from low molecular weight compounds in aqueous solution, but it is also effective for the separation of various compounds having different molecular size.

Inventors

• 👤 Kiyoshi Mizutani

Bibliographic Details