US3882233A
Expired / Lapsed
US
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Methods of increasing production of pituitary hormones
Assignee: Wyeth LLC
•
Filing: 1973-07-27
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Granted: 1975-05-06
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Expires: 1993-07-27
Abstract
Abstract A method for increasing the production of pituitary hormones, including particularly growth hormone, is described which comprises contacting a culture of pituitary cells with an effective amount of cholera enterotoxin.
Inventors
- 👤 Norman H Grant
- 👤 Ruth S Rappaport
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 20:48
CPC Classifications
C; C12; C12N; C12N5/00; C12N5/06; C12N5/0602; C12N5/0613; C12N5/0616; C07; C07K; C07K14/00; C07K14/435; C07K14/575; C07K14/57554; C07K14/59; C07K14/61; C12N2501/00; C12N2501/01
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
40 words
A METHOD OF STIMULATING THE IN VITRO SECRETION OF AT LEAST ONE PITUITARY HORMONE SELECTED FROM THE CLASS CONSISTING OF GROWTH HORMONE, PROLACTIN, LUTEINIZING HORMONE AND THYROTROPIN WHICH COMPRISES CONTACTING CULTURED PITUITARY CELLS WITH AN EFFECTIVE CONCENTRATION OF CHOLERA ENTEROTOXIN.
Claim 2
Independent Claim
40 words
A METHOD OF STIMULATING THE IN VITRO SECRETION OF AT LEAST ONE PITUITARY HORMONE SELECTED FROM THE CLASS CONSISTING OF GROWTH HORMONE, PROLACTIN, LUTEINIZING HORMONE AND THYROTROPIN WHICH COMPRISES CONTACTING CULTURED PITUITARY CELLS WITH AN EFFECTIVE CONCENTRATION OF CHOLERA ENTEROTOXIN.
Claim 3
Dependent Claim
18 words
A method according to claim 1 wherein the minimum cholera enterotoxin concentration is about 1.2 X 10 14M.
Claim 4
Dependent Claim
28 words
A method according to claim 2 which includes the further step of separating and removing said secreted pituitary hormone from said cholera enterotoxin and said cultured pituitary cells.
Claim 5
Dependent Claim
12 words
A method according to claim 1 wherein said pituitary hormone is prolactin.
Claim 6
Dependent Claim
13 words
A method according to claim 1 wherein said pituitary hormone is luteinizing hormone.
Claim 7
Dependent Claim
12 words
A method according to claim 1 wherein said pituitary hormone is thyrotropin.
Claim 8
Independent Claim
30 words
A method of stimulating the in vitro secretion of growth hormone which comprises introducing into cultured pituitary cells cholera enterotoxin at a concentration no less than 1.2 X 10 14M.
Claim 9
Independent Claim
30 words
A method of stimulating the in vitro secretion of growth hormone which comprises introducing into cultured pituitary cells cholera enterotoxin at a concentration no less than 1.2 X 10 14M.
Claim 10
Dependent Claim
21 words
A method according to claim 7 wherein said concentration of said cholera enterotoxin is no less than 1 X 10 11M.
Claim 11
Independent Claim
48 words
A method of increasing the secretion of and recovering a pituitary hormone selected from the class consisting of growth hormone and prolactin which comprises administering by injection to a mammal an effective amount of cholera enterotoxin and then recovering said hormone from the blood plasma of the mammal.
Claim 12
Independent Claim
48 words
A method of increasing the secretion of and recovering a pituitary hormone selected from the class consisting of growth hormone and prolactin which comprises administering by injection to a mammal an effective amount of cholera enterotoxin and then recovering said hormone from the blood plasma of the mammal.
References Cited (Backward Citations)
US3073746A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-05-05 | 1978-11-04 | 1979-05-06 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-05-05 | 1982-11-04 | 1983-05-06 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-05-05 | 1986-11-04 | 1987-05-06 | $7700.00 | Window Closed / Lapsed |