Insoluble carrier-bound enzymes

Claim 2

AN INSOLUBLE ENZYME COMPLEX COMPRISING A NATURAL SPONGE, AN AQUATIC ANIMAL OF THE INVERTEBRATE PHYLUM PORIFERA HAVING A POROUS SKELETON OF HORNY SKELETAL FIBERS AND CONTAINING SPONGIN, WHICH CONSISTS MAINLY OF PROTEIN, TO WHICH IS CHEMICALLY BONDED AN ENZYME.

Claim 3

The enzyme complex of claim 1 wherein the enzyme is bound to the natural sponge by means of a -CO-NH-group.

Claim 4

A process for preparing an insoluble enzyme complex which comprises chemically bonding one or more enzymes to a natural sponge, an aquatic animal of the invertebrate phylum Porifera having a porous skeleton of horny skeletal fibers and containing spongin, which consists mainly of protein.

Claim 5

A process for preparing an insoluble enzyme complex which comprises chemically bonding one or more enzymes to a natural sponge, an aquatic animal of the invertebrate phylum Porifera having a porous skeleton of horny skeletal fibers and containing spongin, which consists mainly of protein.

Claim 6

The process of claim 3 wherein the enzyme is chemically bonded to the sponge by means of a -CO-NH-group.

Claim 7

The process of claim 4 wherein the -CO-NH-group is formed by reacting the sponge with the enzyme in the presence of an organic carbodiimide.

Claim 8

A process for preparing an insoluble enzyme complex which comprises the stePs of: a. modifying a natural sponge by treating it with a cyclic anhydride, said sponge being an aquatic animal of the invertebrate phylum Porifera having a porous skeleton of horny skeletal fibers and containing spongin, which consists mainly of protein; and b. chemically bonding the modified sponge obtained in (a) to an enzyme by means of a -CO-NH-bond.

Claim 9

A process for preparing an insoluble enzyme complex which comprises the stePs of: a. modifying a natural sponge by treating it with a cyclic anhydride, said sponge being an aquatic animal of the invertebrate phylum Porifera having a porous skeleton of horny skeletal fibers and containing spongin, which consists mainly of protein; and b. chemically bonding the modified sponge obtained in (a) to an enzyme by means of a -CO-NH-bond.

Claim 10

A process for preparing an insoluble enzyme complex which comprises the steps of: a. modifying a natural sponge by treating it with a cyclic dicarboxylic acid anhydride, said sponge being an aquatic animal of the invertebrate phylum Porifera having a porous skeleton of horny skeletal fibers and containing spongin, which consists mainly of protein; b. reacting the modified sponge obtained in (a) with a diamine; c. further reacting the product obtained in (b) with a cyclic dicarboxylic acid anhyride; and d. reacting the modified sponge obtained in (c) with an enzyme.

Claim 11

A process for preparing an insoluble enzyme complex which comprises the steps of: a. modifying a natural sponge by treating it with a cyclic dicarboxylic acid anhydride, said sponge being an aquatic animal of the invertebrate phylum Porifera having a porous skeleton of horny skeletal fibers and containing spongin, which consists mainly of protein; b. reacting the modified sponge obtained in (a) with a diamine; c. further reacting the product obtained in (b) with a cyclic dicarboxylic acid anhyride; and d. reacting the modified sponge obtained in (c) with an enzyme.

Claim 12

A process for preparing an insoluble enzyme complex which comprises the steps of: a. modifying a natural sponge with hydrazine whereby a portion of the -CO-NH-bonds of the sponge are converted to -CO-NH-NH2 groups, said sponge being an aquatic animal of the invertebrate phylum Porifera having a porous skeleton of horny skeletal fibers and containing spongin, which consists mainly of protein; b. treating the modified sponge obtained in (a) with a cyclic dicarboxylic acid anhydride, c. further treating the sponge obtained in (b) with a diamine in the presence of a carbodiimide; d. further treating the modified sponge obtained in (c) with cyclic dicarboxylic acid anhydride; and e. treating the sponge obtained in (d) with an enzyme to provide the insoluble enzyme complex.

Claim 13

A process for preparing an insoluble enzyme complex which comprises the steps of: a. modifying a natural sponge with hydrazine whereby a portion of the -CO-NH-bonds of the sponge are converted to -CO-NH-NH2 groups, said sponge being an aquatic animal of the invertebrate phylum Porifera having a porous skeleton of horny skeletal fibers and containing spongin, which consists mainly of protein; b. treating the modified sponge obtained in (a) with a cyclic dicarboxylic acid anhydride, c. further treating the sponge obtained in (b) with a diamine in the presence of a carbodiimide; d. further treating the modified sponge obtained in (c) with cyclic dicarboxylic acid anhydride; and e. treating the sponge obtained in (d) with an enzyme to provide the insoluble enzyme complex.