US3950222A
Expired / Lapsed
US
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Method of immobilizing enzymes to microbial cells
Assignee: National Institute of Advanced Industrial Science and Technology
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Filing: 1974-03-01
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Granted: 1976-04-13
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Expires: 1994-03-01
Abstract
Abstract Microorganic cells or microorganic cells which have undergone various forms of treatments, when treated with a reagent having two or mroe functional groups, give rise to microorganic cells having one of the functional groups of the said reagent chemically bonded thereto and also retaining a free functional group. Contact of an enzyme with the microorganic cells having a free functional group results in immobilization of the enzyme on the said cells.
Inventors
- 👤 Yoshiyuki Takasaki
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 16:53
CPC Classifications
C; C12; C12P; C12P19/00; C12P19/20; C12N; C12N11/00; C12N11/16; C12P19/24
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
59 words
A method for the immobilization of an enzyme on microorganic cells which comprises the steps of first treating said microorganic cells with a di-isocyanate reagent to produce microorganic cells each having a free functional group, and only thereafter reacting the microorganic cells having free functional groups thus obtained with an enzyme to immobilized said enzyme on said microorganic cells.
Claim 2
Independent Claim
59 words
A method for the immobilization of an enzyme on microorganic cells which comprises the steps of first treating said microorganic cells with a di-isocyanate reagent to produce microorganic cells each having a free functional group, and only thereafter reacting the microorganic cells having free functional groups thus obtained with an enzyme to immobilized said enzyme on said microorganic cells.
Claim 3
Dependent Claim
21 words
The method defined in claim 1, wherein the quantity of di-isocyanate reagents used is 1-4 times that of the microorganic cells.
Claim 4
Independent Claim
105 words
A method of making microorganic immobilized-enzyme cells comprising the steps of: a. first reacting microorganic cells selected from the group which consists of mold and Actinomycetes cells with a di-isocyanate to affix said di-isocyanate with one of its functional groups to said cells while leaving another functional group of said di-isocyanate available for reaction; and b. only thereafter contacting the cells having said di-isocyanate affixed thereto with an enzyme reactive with said other group and selected from the group which consists of glucose isomerase, glucoamylase, α-amylase, β-amylase, isoamylase, pullulanase, invertase, galactosidase, cellulase, lipase, protease, glucose oxidase and catalase to immobilize said enzyme on said cells.
Claim 5
Independent Claim
105 words
A method of making microorganic immobilized-enzyme cells comprising the steps of: a. first reacting microorganic cells selected from the group which consists of mold and Actinomycetes cells with a di-isocyanate to affix said di-isocyanate with one of its functional groups to said cells while leaving another functional group of said di-isocyanate available for reaction; and b. only thereafter contacting the cells having said di-isocyanate affixed thereto with an enzyme reactive with said other group and selected from the group which consists of glucose isomerase, glucoamylase, α-amylase, β-amylase, isoamylase, pullulanase, invertase, galactosidase, cellulase, lipase, protease, glucose oxidase and catalase to immobilize said enzyme on said cells.
Claim 6
Independent Claim
41 words
The method defined in claims 3 wherein said cells are selected from the group which consists of Streptomyces, Aspergillus, Rhizopus and Penicillium and said di-isocyanate is selected from the group which consists of tolylene-di-isocyanate, tolylene-2, 4-di-isocyanate, xylene-di-isocyanate, m-xylene- di-isocyanate, and toluene-2-isocyanate-4-isothiocyanate.
Claim 7
Dependent Claim
11 words
The method defined in claim 3 wherein said di-isocyanate is tolylenediisocyanate.
Claim 8
Dependent Claim
19 words
The method defined in claim 5 wherein said cells are cells containing glucose isomerase and said enzyme is glucoamylase.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1979-04-13 | 1979-10-13 | 1980-04-13 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1983-04-13 | 1983-10-13 | 1984-04-13 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1987-04-13 | 1987-10-13 | 1988-04-13 | $7700.00 | Window Closed / Lapsed |