US3971702A
Expired / Lapsed
US
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Diagnostic composition for saccharide determination
Assignee: Shionogi and Co Ltd
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Filing: 1974-12-27
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Granted: 1976-07-27
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Expires: 1994-12-27
Abstract
Abstract A diagnostic composition is prepared which contains as a chromogen a diamino-biphenyl derivative in which at least one of the hydrogen atoms in the amino groups thereof is replaced by a lower alkyl group by reaction with an alkylating agent. A diagnosing instrument is prepared by impregnating a bibulous, porous support in a solution comprising the diagnostic composition and optionally a saccharide oxidase, peroxidase and a guaiac, and, if necessary, a buffer, a surfactant, a background adjusting dye and/or an agent for increasing the viscosity of the composition. This diagnosing instrument can be handled in a simple and convenient way and is used to identify semi-quantitatively a saccharide in body fluids or excrements of humans or animals with reliability.
Inventors
- 👤 Hideyuki Maekawa
- 👤 Kaoru Ishitobi
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 23:18
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/26; C12Q1/28; C12Q1/54; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/52; C12Q2326/00; C12Q2326/10; C12Q2326/14; Y; Y10; Y10S; Y10S435/00; Y10S435/805
Claims Analysis
Total Claims: 42
Claim 1
Independent Claim
55 words
A diagnostic composition comprising as a chromogen a diamino-biphenyl derivative having alkyl substituents in the phenyl groups thereof and in which at least one of the hydrogen atoms in either of the amino groups thereof is substituted by at least one lower alkyl group having from one to four carbon atoms and a saccharide oxidase.
Claim 2
Dependent Claim
55 words
A diagnostic composition comprising as a chromogen a diamino-biphenyl derivative having alkyl substituents in the phenyl groups thereof and in which at least one of the hydrogen atoms in either of the amino groups thereof is substituted by at least one lower alkyl group having from one to four carbon atoms and a saccharide oxidase.
Claim 3
Dependent Claim
19 words
The diagnostic composition according to claim 1, wherein the lower alkyl group has from one to two carbon atoms.
Claim 4
Dependent Claim
13 words
The diagnostic composition according to claim 1, wherein the diamino-biphenyl derivative is tolidine.
Claim 5
Dependent Claim
12 words
The diagnostic composition according to claim 3, wherein the tolidine is ortho-tolidine.
Claim 6
Dependent Claim
17 words
The diagnostic composition according to claim 1, wherein the diamino-biphenyl derivative is N-methyl-, N,N-dimethyl-, N,N'-dimethyl-, N,N,N'trimethyl-or N,N,N',N'-tetramethyl-ortho-tolidine.
Claim 7
Dependent Claim
19 words
The diagnostic composition according to claim 1, wherein the alkylated diamino-biphenyl derivative is N-ethyl-, N,N-diethyl-, N,N'-diethyl-, N,N,N'-triethyl- or N,N,N',N'-tetraethyl-ortho-tolidine.
Claim 8
Dependent Claim
24 words
The diagnostic composition according to claim 1, wherein the diamino-biphenyl derivative has up to four lower alkyl substituents in its amino group or groups.
Claim 9
Dependent Claim
21 words
The diagnostic composition according to claim 1, wherein said diamino-biphenyl derivative is used in a mixture with a non-alkylated diamino-biphenyl derivative.
Claim 10
Dependent Claim
17 words
The diagnostic composition according to claim 1, wherein said composition additionally contains a peroxidase and a guaiac.
Claim 11
Dependent Claim
17 words
The diagnostic composition according to claim 9, wherein the saccharide oxidase is glucose oxidase or galactose oxidase.
Claim 12
Dependent Claim
16 words
The diagnostic composition according to claim 9, wherein the guaiac is alphaguaicaconic acid or betaquaiaconic acid.
Claim 13
Dependent Claim
28 words
The diagnostic composition according to claim 1, wherein said composition additionally contains a buffer, a surfactant, a background adjusting dye and an agent for increasing the viscosity thereof.
Claim 14
Dependent Claim
22 words
The diagnostic composition according to claim 12, wherein the buffer is an aqueous solution of phthalic acid, a phosphate or citric acid.
Claim 15
Dependent Claim
14 words
The diagnostic composition according to claim 12, wherein the background adjusting dye is tartrazine.
Claim 16
Dependent Claim
18 words
The diagnostic composition according to claim 12, wherein the agent for increasing the viscosity is a protecting colloid.
Claim 17
Dependent Claim
17 words
The diagnostic composition according to claim 15, wherein the protecting colloid is polyalkylene glycol or polyvinyl alcohol.
Claim 18
Dependent Claim
48 words
A diagnosing instrument comprising a diamino-biphenyl derivative having alkyl substitutents in the phenyl groups thereof and which is substituted by at least one alkyl group having from one to four carbon atoms at either of its amino groups and a saccharide oxidase, supported on a bibulous, porous support.
Claim 19
Dependent Claim
48 words
A diagnosing instrument comprising a diamino-biphenyl derivative having alkyl substitutents in the phenyl groups thereof and which is substituted by at least one alkyl group having from one to four carbon atoms at either of its amino groups and a saccharide oxidase, supported on a bibulous, porous support.
Claim 20
Dependent Claim
19 words
The diagnosing instrument according to claim 17, wherein the lower alkyl group has from one to two carbon atoms.
Claim 21
Dependent Claim
13 words
The diagnosing instrument according to claim 17, wherein the diamino-biphenyl derivative is tolidine.
Claim 22
Dependent Claim
12 words
The diagnosing instrument according to claim 19, wherein the tolidine is ortho-tolidine.
Claim 23
Dependent Claim
12 words
The diagnosing instrument according to claim 19, wherein the dianisidine is ortho-dianisidine.
Claim 24
Dependent Claim
19 words
The diagnosing instrument according to claim 17, wherein the diamino-biphenyl derivative is N-methyl-, N,N'-dimethyl-, N,N,N'-trimethyl- or N,N,N' ,N'-tetramethyl- ortho-tolidine.
Claim 25
Dependent Claim
19 words
The diagnosing instrument according to claim 17, wherein the alkylated diamino-biphenyl derivative is N-ethyl-, N,N-diethyl-, N,N'-diethyl-, N,N,N'-triethyl- or N,N,N,',N'-tetraethyl-ortho-tolidine.
Claim 26
Dependent Claim
24 words
The diagnosing instrument according to claim 17, wherein the diamino-biphenyl derivative has up to four lower alkyl substituents in its amino group or groups.
Claim 27
Dependent Claim
21 words
The diagnosing instrument according to claim 17, wherein said diamino-biphenyl derivative is used in a mixture with a non-alkylated diamino-biphenyl derivative.
Claim 28
Dependent Claim
17 words
The diagnosing instrument according to claim 17, wherein said composition additionally contains a peroxidase and a guaiac.
Claim 29
Dependent Claim
17 words
The diagnosing instrument according to claim 17, wherein the saccharide oxidase is glucose oxidase or galactose oxidase.
Claim 30
Dependent Claim
16 words
The diagnosing instrument according to claim 17, wherein the guaiac is alphaguaiaconic acid or betaguaiaconic acid.
Claim 31
Dependent Claim
28 words
The diagnosing instrument according to claim 17, wherein said composition additionally contains a buffer, a surfactant, a background adjusting dye and an agent for increasing the viscosity thereof.
Claim 32
Dependent Claim
22 words
The diagnosing instrument according to claim 17, wherein the buffer is an aqueous solution of phthalic acid, a phosphate or citric acid.
Claim 33
Dependent Claim
14 words
The diagnosing instrument according to claim 17, wherein the background adjusting dye is tartrazine.
Claim 34
Dependent Claim
18 words
The diagnosing instrument according to claim 17, wherein the agent for increasing the viscosity is a protecting colloid.
Claim 35
Dependent Claim
17 words
The diagnosing instrument according to claim 32, wherein the protecting colloid is polyalkylene glycol or polyvinyl alcohol.
Claim 36
Dependent Claim
27 words
The diagnosing instrument according to claim 17, wherein the bibulous, porous support is filter paper, hardened filter paper, sintered porous glass material or sintered porous plastic material.
Claim 37
Dependent Claim
36 words
The diagnosing instrument according to claim 26, wherein the bibulous, porous support is impregnated in a solution comprising the saccharide oxidase and peroxidase and subsequently in another solution comprising the alkylated diamino-biphenyl derivative and the quaiac.
Claim 38
Independent Claim
66 words
A method for manufacturing a diagnosing instrument which comprises impregnating a bibulous, porous support in a solution comprising a diamino-biphenyl derivative having alkyl substituents in the phenyl groups thereof and in which at least one of the hydrogen atoms in either of the amino groups thereof is substituted by at least one lower alkyl group having from one to four carbon atoms and a saccharide oxidase.
Claim 39
Independent Claim
66 words
A method for manufacturing a diagnosing instrument which comprises impregnating a bibulous, porous support in a solution comprising a diamino-biphenyl derivative having alkyl substituents in the phenyl groups thereof and in which at least one of the hydrogen atoms in either of the amino groups thereof is substituted by at least one lower alkyl group having from one to four carbon atoms and a saccharide oxidase.
Claim 40
Dependent Claim
33 words
The method according to claim 36, wherein the diamino-biphenyl derivative is benzidine with or without having at least one substituent in the alkyl or alkoxy series with from one to two carbon atoms.
Claim 41
Dependent Claim
33 words
The method according to claim 36, wherein said bibulous, porous support is impregnated in a solution containing a saccharide oxidase and peroxidase and in a solution containing said diamino-biphenyl derivative and a guaiac.
Claim 42
Dependent Claim
12 words
The method according to claim 36, wherein the diamino-biphenyl derivative is ortho-tolidine.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1979-07-27 | 1980-01-26 | 1980-07-27 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1983-07-27 | 1984-01-26 | 1984-07-27 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1987-07-27 | 1988-01-26 | 1988-07-27 | $7700.00 | Window Closed / Lapsed |