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US3979263A Expired / Lapsed US ⏱️ 0.0 years remaining

Method for growing cultures in a body cavity in the presence of gas

Assignee: Microbyx Corp
•
Filing: 1974-06-12
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Granted: 1976-09-07
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Expires: 1994-06-12
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Abstract

Abstract Cultures of microorganisms can be grown in a body cavity by situating therein a nutrient on a suitable carrier while maintaining the nutrient exposed to a predetermined gaseous atmosphere, so that microorganisms which require a particular atmosphere can grow directly in the body cavity on the nutrient. After a time sufficient for culture growth the carrier with the nutrient thereon are removed from the body cavity and inspected, for example, under a microscope with suitable dyes being used to stain the growth for rendering the latter easily visible.

Inventors

  • 👤 Louis Bucalo

Bibliographic Details

Application Number —
Jurisdiction US
Data Source google_patents
Last Synchronized 2026-11-05 00:37
CPC Classifications
A; A61; A61B; A61B10/00; A61B10/02; A61F; A61F13/00; A61F13/15; A61F13/20; A61F13/2051; A61F13/206

Claims Analysis

Total Claims: 17
Claim 1
Independent Claim 132 words
In a culturing method, the steps of supporting a nutrient for a microorganism on a carrier for the nutrient, situating the carrier with the nutrient thereon in a body cavity which has a given atmosphere while contacting the nutrient with a body fluid which may have therein a microorganism which will grow in the nutrient, modifying said atmosphere for said microorganism to facilitate the growth of said microorganism, maintaining the nutrient while it is in the body cavity exposed to said modified atmosphere so that a microorganism will grow in the nutrient, and, after a given time sufficient for growth of the microorganism in the nutrient adequate to render the microorganism visible, removing the carrier with the nutrient thereon from the body cavity and then checking for the presence of the microorganism.
Claim 2
Dependent Claim 132 words
In a culturing method, the steps of supporting a nutrient for a microorganism on a carrier for the nutrient, situating the carrier with the nutrient thereon in a body cavity which has a given atmosphere while contacting the nutrient with a body fluid which may have therein a microorganism which will grow in the nutrient, modifying said atmosphere for said microorganism to facilitate the growth of said microorganism, maintaining the nutrient while it is in the body cavity exposed to said modified atmosphere so that a microorganism will grow in the nutrient, and, after a given time sufficient for growth of the microorganism in the nutrient adequate to render the microorganism visible, removing the carrier with the nutrient thereon from the body cavity and then checking for the presence of the microorganism.
Claim 3
Dependent Claim 57 words
In a method as recited in claim 1 and including the step of preliminarily packaging the carrier with the nutrient thereon in a sterile package which seals in the interior of the package an amount of moisture sufficient to prevent drying of the nutrient prior to placing of the latter with the carrier in the body cavity.
Claim 4
Dependent Claim 46 words
In a method as recited in claim 1 and including the step of placing the carrier with the nutrient thereon in the vagina to check for microorganisms associated with gonorrhea, vaginitis, or other diseases occurring in the vagina and involving microoganisms which grow, in said atmosphere.
Claim 5
Dependent Claim 32 words
In a method as recited in claim 1 and including the step of placing the carrier with the nutrient thereon in a male urethra to check for a disease such as gonnorrhea.
Claim 6
Dependent Claim 44 words
In a method as recited in claim 1 and including the step of incorporating the carrier with the nutrient thereon into a tampon and then introducing the tampon into a body cavity so that a fluid absorbed by the tampon will engage the nutrient.
Claim 7
Dependent Claim 41 words
In a method as recited in claim 5 and including the step of situating a plurality of carriers with different nutrients thereon, respectively, in the tampon so that a check may be simultaneously carried out for a number of different microorganisms.
Claim 8
Dependent Claim 128 words
In a method as recited in claim 1 and wherein a plurality of different nutrients for different microorganisms, respectively, are respectively supported on different carriers which are simultaneously situated with the nutrients thereon in a body cavity while contacting the plurality of nutrients with a body fluid which may have therein the different microorganisms which will grow in the different nutrients, respectively, maintaining the different nutrients while in the body cavity exposed to said modified atmosphere so that microorganisms will grow in the nutrients, and, after a time sufficient for growth of the microorganisms in the nutrients adequate to render the microorganisms visible, removing the plurality of carriers with the nutrients thereon from the body cavity and checking for the presence of the microorganisms in the nutrients, respectively.
Claim 9
Dependent Claim 33 words
In a method as recited in claim 1 and wherein the nutrient is supported on the carrier with a varying degree of density to encourage grown of the microorganism in a predetermined direction.
Claim 10
Dependent Claim 62 words
In a method as recited in claim 1 and wherein the carrier with the nutrient thereon is coiled while situated in the body cavity and uncoiled when removed from the body cavity, and including the steps of placing opposed ends of the carrier into grooves at opposed ends of a flat plate for holding the carrier flat during checking of the nutrient.
Claim 11
Dependent Claim 19 words
In a method as recited in claim 1 and wherein the microorganism is aerobic, said modified atmosphere being air.
Claim 12
Dependent Claim 22 words
In a method as recited in claim 1 and wherein said microorganism is anaerobic, and said modified atmosphere having no oxygen therein.
Claim 13
Dependent Claim 15 words
In a method as recited in claim 11 and wherein said modified atmosphere is nitrogen.
Claim 14
Dependent Claim 26 words
In a method as recited in claim 1 and wherein said modified atmosphere is carbon dioxide while said microorganism grows best in a carbon dioxide atmosphere.
Claim 15
Dependent Claim 31 words
In a method as recited in claim 13 and including the step of generating the carbon dioxide atmosphere while the carrier with the nutrient thereon is situated in the body cavity.
Claim 16
Dependent Claim 29 words
In a method as recited in claim 1 and including the step of generating said modified atmosphere while the carrier with the nutrient thereon is in the body cavity.
Claim 17
Dependent Claim 36 words
In a method as recited in claim 1 and including the step of preliminarily packaging the carrier with the nutrient thereon in a sterile package which seals said given atmosphere in the interior of said package.

References Cited (Backward Citations)

US3842166A Cited
US3864213A Cited

USPTO Annuity & Maintenance Windows

Annuity Stage Window Opens Payment Deadline Surcharge Period Until Estimated Fee Status
3.5 Years (Year 4) 1979-09-07 1980-03-08 1980-09-07 $2000.00 Window Closed / Lapsed
7.5 Years (Year 8) 1983-09-07 1984-03-08 1984-09-07 $3760.00 Window Closed / Lapsed
11.5 Years (Year 12) 1987-09-07 1988-03-08 1988-09-07 $7700.00 Window Closed / Lapsed

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