US4001087A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Affinity labelling enzymes with esters of aromatic sulfonic acids
Assignee: Government of the United States of America
•
Filing: 1975-11-05
•
Granted: 1977-01-04
•
Expires: 1995-11-05
Abstract
Abstract Novel esters of aromatic sulfonic acids are disclosed. The specific esters are nitrophenyl p- and m-amidinophenylmethanesulfonate. Also disclosed is a method for specific inactivation of the enzyme, thrombin, employing nitrophenyl p-amidinophenylmethanesulfonate.
Inventors
- 👤 Show-Chu Wong
- 👤 Elliott Shaw
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 11:01
CPC Classifications
C; C07; C07C; C07C309/00; C07C309/63; C07C309/64; C07C309/67; C07C309/69; C07C309/70
Claims Analysis
Total Claims: 3
Claim 1
Independent Claim
61 words
In a method for the study of enzymes which comprises affinity labelling enzymes for identifying or inhibiting their functional groups, the improvement which comprises reacting a purified enzyme with a compound selected from the group consisting of nitrophenyl p- and m-amidinophenylmethanesulfonate whereby there is obtained covalent bond formation between said compound and said enzyme with inactivation or inhibition of the enzyme.
Claim 2
Dependent Claim
61 words
In a method for the study of enzymes which comprises affinity labelling enzymes for identifying or inhibiting their functional groups, the improvement which comprises reacting a purified enzyme with a compound selected from the group consisting of nitrophenyl p- and m-amidinophenylmethanesulfonate whereby there is obtained covalent bond formation between said compound and said enzyme with inactivation or inhibition of the enzyme.
Claim 3
Dependent Claim
17 words
The method of claim 1 wherein said enzyme is thrombin and wherein said compound is nitrophenyl p-amidinophenylmethanesulfonate.
References Cited (Backward Citations)
US3745088A
Cited
US3839153A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-01-04 | 1980-07-05 | 1981-01-04 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-01-04 | 1984-07-05 | 1985-01-04 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-01-04 | 1988-07-05 | 1989-01-04 | $7700.00 | Window Closed / Lapsed |