US4012287A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method and reagent for the quantitative analysis of triglycerides
Assignee: Hach Lange GmbH
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Filing: 1975-11-18
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Granted: 1977-03-15
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Expires: 1995-11-18
Abstract
Abstract A reagent and method for the quantitative of serum triglycerides which involves the alcoholic saponification of serum lipides using a low molecular weight chlorinated hydrocarbon as a catalyst. The saponified lipid is then mixed with an enzyme preparation for hydrolysis. After hydrolysis, the triglyceride is quantitatively analyzed in a conventional manner.
Inventors
- 👤 Brigitte Carl
- 👤 Jaroslav Stourac
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 10:02
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/48; C12Q1/61
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
28 words
A reagent for the quantitative analysis of triglycerides comprising: an alcoholic solution of potassium hydroxide, an aqueous solution of magnesium sulfate, and a low molecular weight chlorinated hydrocarbon.
Claim 2
Dependent Claim
28 words
A reagent for the quantitative analysis of triglycerides comprising: an alcoholic solution of potassium hydroxide, an aqueous solution of magnesium sulfate, and a low molecular weight chlorinated hydrocarbon.
Claim 3
Dependent Claim
11 words
The reagent of claim 1 wherein said chlorinated hydrocarbon is trichloromethane.
Claim 4
Dependent Claim
16 words
The reagent of claim 2 wherein said trichloromethane constitutes about 17% by weight of said reagent.
Claim 5
Dependent Claim
55 words
In a method for the quantitative analysis of triglycerides by enzyme hydrolysis of said triglyceride in an alkaline aqueous medium to form glycerol, followed by quantitative analysis of the glycerol, the improvement which consists of adding a catalytic amount of a low molecular weight chlorinated hydrocarbon to the hydrolysis medium to accelerate the hydrolytic cleavage.
Claim 6
Dependent Claim
55 words
In a method for the quantitative analysis of triglycerides by enzyme hydrolysis of said triglyceride in an alkaline aqueous medium to form glycerol, followed by quantitative analysis of the glycerol, the improvement which consists of adding a catalytic amount of a low molecular weight chlorinated hydrocarbon to the hydrolysis medium to accelerate the hydrolytic cleavage.
Claim 7
Dependent Claim
14 words
The method of claim 4 wherein said low molecular weight chlorinated hydrocarbon is trichloromethane.
Claim 8
Dependent Claim
27 words
The method of claim 4 wherein said trichloromethane is added in an amount of about 20% by weight, based on the amount of the alkaline hydrolysis medium.
References Cited (Backward Citations)
US3645688A
Cited
US3703591A
Cited
US3791791A
Cited
US3862009A
Cited
US3894844A
Cited
US3898130A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-03-14 | 1980-09-13 | 1981-03-15 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-03-14 | 1984-09-13 | 1985-03-15 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-03-14 | 1988-09-13 | 1989-03-15 | $7700.00 | Window Closed / Lapsed |