US4021535A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Reagents used in the radioimmunoassay of digoxin
Assignee: Beckman Coulter Inc
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Filing: 1975-01-14
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Granted: 1977-05-03
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Expires: 1995-01-14
Abstract
Abstract The derivatives of digoxin useful in the radioimmunoassay of digoxin having the following formulae: ##STR1## where R is a carboxylic acid ester. These derivatives are formed by the following process: (1) reacting the carboxylic acid in the presence of trifluoroacetic anhydride with a solution of digoxin in an inert, nonaqueous solvent medium, (2) adding water so as to form the above derivatives, (3) purifying the above derivatives from other constituents of the reaction mixture and (4) radio-labeling these derivatives for use as a labeled hapten in the radioimmunoassay of digoxin. The preferred carboxylic acids are imidazoleacetic acid and p-hydroxyphenylpropionic acid. The preferred radioactive isotope is 125 I.
Inventors
- 👤 Alan J. Polito
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 12:33
CPC Classifications
C; C07; C07J; C07J19/00; C07J19/005; C07J43/00; C07J43/003; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/58; G01N33/60; Y; Y10; Y10S; Y10S436/00; Y10S436/815; Y10S436/817
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
15 words
A radioimmunoassay reagent having the structural formulae: ##STR9## wherein the asterisk * indicates radioactive labelling.
Claim 2
Dependent Claim
15 words
A radioimmunoassay reagent having the structural formulae: ##STR9## wherein the asterisk * indicates radioactive labelling.
Claim 3
Dependent Claim
14 words
The radioimmunoassay reagent of claim 1 wherein the radioactive labelling is with 125 I.
Claim 4
Dependent Claim
36 words
In a method for the determination of quantities digoxin in blood by radioimmunoassay, the improvement comprising the use of a compound having the following formulae as the radioimmunoassay reagent: ##STR10## wherein the asterisk* indicates radioactive labelling.
Claim 5
Dependent Claim
36 words
In a method for the determination of quantities digoxin in blood by radioimmunoassay, the improvement comprising the use of a compound having the following formulae as the radioimmunoassay reagent: ##STR10## wherein the asterisk* indicates radioactive labelling.
Claim 6
Dependent Claim
15 words
The method of claim 3 wherein the radioimmunoassay reagent is radioactively labelled with 125 I.
Claim 7
Dependent Claim
15 words
A radioimmunoassay reagent having the structural formulae: ##STR11## wherein the asterisk * indicates radioactive labelling.
Claim 8
Dependent Claim
15 words
A radioimmunoassay reagent having the structural formulae: ##STR11## wherein the asterisk * indicates radioactive labelling.
Claim 9
Dependent Claim
14 words
The radioimmunoassay reagent of claim 5 wherein the radioactive labelling is with 125 I.
Claim 10
Dependent Claim
37 words
In a method for the determination of quantities digoxin in blood by radioimmunoassay, the improvement comprising the use of a compound having the following formulae as the radioimmunoassay reagent: ##STR12## wherein the asterisk * indicates radioactive labelling.
Claim 11
Dependent Claim
37 words
In a method for the determination of quantities digoxin in blood by radioimmunoassay, the improvement comprising the use of a compound having the following formulae as the radioimmunoassay reagent: ##STR12## wherein the asterisk * indicates radioactive labelling.
Claim 12
Dependent Claim
15 words
The method of claim 7 wherein the radioimmunoassay reagent is radioactively labelled with 125 I.
Claim 13
Independent Claim
104 words
The method of making a radioimmunoassay or other competitive-protein assays derivative reagent for digoxin comprising the steps of: (a) reacting a carboxylic acid in the presence of trifluoroacetic anhydride with a solution of digoxin in an inert, nonaqueous solvent medium; (b) then mixing sufficient water into the reaction mixture so as to form derivatives having the following structural formulae: ##STR13## wherein R is an ester of imidazoleacetic acid or p-hydroxyphenylpropionic acid; (c) purifying said derivatives from the other constituents of said reaction mixture; and (d) tagging the derivatives with a radio-isotope so as to radio-label the derivative for use in the radioimmunoassay of digoxin.
Claim 14
Independent Claim
104 words
The method of making a radioimmunoassay or other competitive-protein assays derivative reagent for digoxin comprising the steps of: (a) reacting a carboxylic acid in the presence of trifluoroacetic anhydride with a solution of digoxin in an inert, nonaqueous solvent medium; (b) then mixing sufficient water into the reaction mixture so as to form derivatives having the following structural formulae: ##STR13## wherein R is an ester of imidazoleacetic acid or p-hydroxyphenylpropionic acid; (c) purifying said derivatives from the other constituents of said reaction mixture; and (d) tagging the derivatives with a radio-isotope so as to radio-label the derivative for use in the radioimmunoassay of digoxin.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-05-02 | 1980-11-01 | 1981-05-03 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-05-02 | 1984-11-01 | 1985-05-03 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-05-02 | 1988-11-01 | 1989-05-03 | $7700.00 | Window Closed / Lapsed |