US4025389A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process and isomerizing glucose
Assignee: Novo Nordisk AS
•
Filing: 1976-02-27
•
Granted: 1977-05-24
•
Expires: 1996-02-27
Abstract
Abstract Continuous enzymatic isomerization of a glucose syrup with a glucose concentration of 30-55% by weight containing less than about 10 - 3 M calcium, less than about 10 - 2 M of Mg + + , the Mg + + being in a concentration, whereby the molar ratio of magnesium to calcium is greater than 5, the isomerization taking place at a pH 7.8-8.6 with a total contact time between enzyme and syrup less than about 3.5 hours, preferably less than 2 hours. A convenient temperature range for isomerization is 60°-85° C., preferably 60°-70° C. The syrup has no colbalt added thereto. A preferred practice involves a syrup with very little added magnesium. Post isomerization ion exchange treatment can be avoided. The enzyme is a particulate preparation derived from B. coagulans, preferably by glutaraldehyde reaction with homogenized microorganism cells.
Inventors
- 👤 Poul Borge Rosenius Poulsen
- 👤 Lena Elisabeth Zittan
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 19:52
CPC Classifications
C; C13; C13K; C13K11/00; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/832
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
140 words
A continuous process for isomerizing glucose syrup to a glucose fructose mixture which comprises passing a 30-55% by weight of a starch hydrolysate glucose syrup which contains not more than 10 - 3 M Ca + + and 10 - 2 M Mg + + , and where the molar ratio Mg + +:Ca + + is between 5-500:1 for Mg + + > 10 - 3 M, through a bed of glutaraldehyde immobilized and cross-linked glucose isomerase particles exceeding about 100 microns derived from the reaction of ruptured cells of B. coagulans with glutaraldehyde at an inlet pH in the range of pH 7.8-8.6 at temperatures in the range of 60°-85° C. for a total contact time of less than 3.5 hours, and isomerizing the syrup thereby to at least 40% fructose by weight of the glucose fructose content.
Claim 2
Dependent Claim
140 words
A continuous process for isomerizing glucose syrup to a glucose fructose mixture which comprises passing a 30-55% by weight of a starch hydrolysate glucose syrup which contains not more than 10 - 3 M Ca + + and 10 - 2 M Mg + + , and where the molar ratio Mg + +:Ca + + is between 5-500:1 for Mg + + > 10 - 3 M, through a bed of glutaraldehyde immobilized and cross-linked glucose isomerase particles exceeding about 100 microns derived from the reaction of ruptured cells of B. coagulans with glutaraldehyde at an inlet pH in the range of pH 7.8-8.6 at temperatures in the range of 60°-85° C. for a total contact time of less than 3.5 hours, and isomerizing the syrup thereby to at least 40% fructose by weight of the glucose fructose content.
Claim 3
Dependent Claim
16 words
The process of claim 1 wherein added Co + + is absent from the glucose syrup.
Claim 4
Dependent Claim
50 words
The process of claim 1 wherein the Ca + + is less than about 2.5 × 10 - 5 M and the Mg + + is less than about 5 × 10 - 4 M, the Mg + + content being at least 10 times the Ca + + content.
Claim 5
Dependent Claim
16 words
The process of claim 2 wherein the particulate glucose isomerase enzyme is disposed in a column.
Claim 6
Dependent Claim
15 words
The process of claim 1 wherein the contact time is less than about 2 hours.
Claim 7
Dependent Claim
19 words
The process of claim 1 which comprises conducting the process with a syrup containing 40-45% by weight of glucose.
Claim 8
Dependent Claim
18 words
The process of claim 1 which comprises conducting the process at a temperature between 60° and 70° C.
Claim 9
Dependent Claim
30 words
The process of claim 1 wherein the B. coagulans is an atypical strain of B. coagulans characterized by the ability to grow solely on inorganic nitrogen as the nitrogen nutrient.
Claim 10
Dependent Claim
29 words
The process of claim 1 wherein the molar ratio Mg + +:Ca + + exceeds 5:1 when Mg + + content is not more that 10 - 3 M.
References Cited (Backward Citations)
US3753858A
Cited
US3694314A
Cited
US3779869A
Cited
US3821086A
Cited
US3979261A
Cited
US3843442A
Cited
US3868304A
Cited
US3980521A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-05-23 | 1980-11-22 | 1981-05-24 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-05-23 | 1984-11-22 | 1985-05-24 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-05-23 | 1988-11-22 | 1989-05-24 | $7700.00 | Window Closed / Lapsed |