US4025390A
Expired / Lapsed
US
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Method for recovering urokinase from a urokinase-containing solution
Assignee: Resonac Corp
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Filing: 1976-04-13
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Granted: 1977-05-24
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Expires: 1996-04-13
Abstract
Abstract A method for recovering urokinase which comprises bringing a urokinase-containing solution into contact with at least one compound selected from a group consisting of cyanoalkyl polysaccharide, cyanoalkyl modified-polysaccharide and cyanoalkyl polyvinyl alcohol to subject urokinase to adsorption and then eluting the urokinase from the adsorbate.
Inventors
- 👤 Masaharu Urakawa
- 👤 Hiroshi Sumiyama
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 20:29
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/48; C12N9/50; C12N9/64; C12N9/6421; C12N9/6424; C12N9/6456; C12N9/6462; C12Y; C12Y304/00; C12Y304/21; C12Y304/21073; Y; Y10; Y10S; Y10S435/00; Y10S435/814; Y10S435/815
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
67 words
A method for recovering urokinase which comprises bringing a urokinase-containing solution into contact with at least one compound selected from the group consisting of cyanoalkyl polysaccharide, cyanoalkyl modified-polysaccharide and cyanoalkyl polyvinyl alcohol to subject urokinase to adsorption and then eluting the urokinase from the adsorbate, said cyanoalkyl group being a cyano-lower-alkyl group having from 2 to 4 carbon atoms including the carbon atom of the cyano group.
Claim 2
Independent Claim
67 words
A method for recovering urokinase which comprises bringing a urokinase-containing solution into contact with at least one compound selected from the group consisting of cyanoalkyl polysaccharide, cyanoalkyl modified-polysaccharide and cyanoalkyl polyvinyl alcohol to subject urokinase to adsorption and then eluting the urokinase from the adsorbate, said cyanoalkyl group being a cyano-lower-alkyl group having from 2 to 4 carbon atoms including the carbon atom of the cyano group.
Claim 3
Dependent Claim
18 words
The method of claim 1, wherein said polysaccharide and modified-polysaccharide are cellulose, dextran and dextran cross-linked with epichlorohydrin.
Claim 4
Dependent Claim
11 words
The method of claim 1, wherein said cyano-lower-alkyl group is cyanoethyl.
Claim 5
Dependent Claim
20 words
The method of claim 1, wherein said adsorbing operation is conducted at a pH range of from 4.5 to 7.5.
Claim 6
Dependent Claim
14 words
The method of claim 4, wherein said pH value is from 5.0 to 6.5.
Claim 7
Dependent Claim
38 words
The method of claim 1, wherein said eluting operation is conducted using for the elution agent an aqueous solution of alkali having such a pH value that the resulting eluate has a pH of from 9 to 11.5.
Claim 8
Dependent Claim
18 words
The method of claim 6, wherein said alkali solution is about 4% by weight aqueous solution of ammonia.
References Cited (Backward Citations)
US3723251A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-05-23 | 1980-11-22 | 1981-05-24 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-05-23 | 1984-11-22 | 1985-05-24 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-05-23 | 1988-11-22 | 1989-05-24 | $7700.00 | Window Closed / Lapsed |