US4029642A
Expired / Lapsed
US
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Process for the manufacture of human insulin
Assignee: Hoechst AG
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Filing: 1975-12-19
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Granted: 1977-06-14
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Expires: 1995-12-19
Abstract
Abstract A process for the manufacture of human insulin by the condensation of porcine des-B 23-30 octapeptide insulin with a peptide and subsequent splitting-off of the protective groups by treatment with trifluoroacetic acid or with alkali.
Inventors
- 👤 Rainer Obermeier
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 10:10
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/575; C07K14/62; Y; Y02; Y02P; Y02P20/00; Y02P20/50; Y02P20/55; Y10; Y10S; Y10S930/00; Y10S930/01; Y10S930/26
Claims Analysis
Total Claims: 2
Claim 1
Independent Claim
77 words
A method for making human insulin, which comprises condensing des-B 23-30 -octapeptide porcine insulin of the formula ##STR3## wherein R is an amino-protective group which is tert.-butyloxy-carbonyl, tert.-amyloxy-carbonyl, or methylsulfonyl-ethyloxy-carbonyl with a peptide of the formula H--Gly--Phe--Phe--Tyr(X)--Thr(X)--Pro--Lys(BOC)--Thr(Bu.sup.t)OBu.sup.t, wherein X is hydrogen or tert.-butyl, in the presence of dicyclohexyl carbodiimide and hydroxybenzotriazole, and then splitting off said amino-protective group with alkali if said group is methylsulfonyl-ethyloxy-carbonyl or with trifluoroacetic acid if said protective group is tert.-butyloxy-carbonyl or tert.-amyloxy-carbonyl.
Claim 2
Independent Claim
77 words
A method for making human insulin, which comprises condensing des-B 23-30 -octapeptide porcine insulin of the formula ##STR3## wherein R is an amino-protective group which is tert.-butyloxy-carbonyl, tert.-amyloxy-carbonyl, or methylsulfonyl-ethyloxy-carbonyl with a peptide of the formula H--Gly--Phe--Phe--Tyr(X)--Thr(X)--Pro--Lys(BOC)--Thr(Bu.sup.t)OBu.sup.t, wherein X is hydrogen or tert.-butyl, in the presence of dicyclohexyl carbodiimide and hydroxybenzotriazole, and then splitting off said amino-protective group with alkali if said group is methylsulfonyl-ethyloxy-carbonyl or with trifluoroacetic acid if said protective group is tert.-butyloxy-carbonyl or tert.-amyloxy-carbonyl.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-06-13 | 1980-12-13 | 1981-06-14 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-06-13 | 1984-12-13 | 1985-06-14 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-06-13 | 1988-12-13 | 1989-06-14 | $7700.00 | Window Closed / Lapsed |