US4034071A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Immunoassay procedures
Assignee: Allegheny General Hospital
•
Filing: 1976-01-26
•
Granted: 1977-07-05
•
Expires: 1996-01-26
Abstract
Abstract The technique of separating a tagged bound material from the same unbound material in immunoassay procedures is improved by utilizing a clathrate sorbent to carry out the separation. A clathrate sorbent, for example, comprises a mixture of hydrate zinc sulfate and sodium hydroxide which form an inclusion compound with charcoal powder. Charcoal powder has also been combined to form a clathrate sorbent with salts of other metals which produce a gelatinous substance with alkaline hydroxides; e.g., alum with sodium hydroxide. Another example of a clathrate sorbent is magnesium trisilicate combined with the aforementioned zinc preparation.
Inventors
- 👤 Herbert S. Strickler
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 09:14
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/536; G01N33/537; G01N33/539; B; B01; B01J; B01J20/00; B01J20/02; B01J20/0203; B01J20/024; B01J20/0244; B01J20/0274; B01J20/0281; B01J20/04; B01J20/041; B01J20/10; B01J20/20; B01J2220/00; B01J2220/40; B01J2220/42; Y; Y10; Y10S; Y10S436/00; Y10S436/804; Y10S436/807; Y10S436/808
Claims Analysis
Total Claims: 19
Claim 1
Independent Claim
66 words
In an immunoassay procedure, including the step of separating within a mixture a bound untagged substance and a bound tagged same substance from unbound substance and unbound tagged same substance by selectively absorbing the unbound portions, the improvement comprising subjecting said mixture to a clathrate compound having a host substance and an inclusion substance to carry out the separation, wherein a sorbent is the host substance.
Claim 2
Dependent Claim
66 words
In an immunoassay procedure, including the step of separating within a mixture a bound untagged substance and a bound tagged same substance from unbound substance and unbound tagged same substance by selectively absorbing the unbound portions, the improvement comprising subjecting said mixture to a clathrate compound having a host substance and an inclusion substance to carry out the separation, wherein a sorbent is the host substance.
Claim 3
Dependent Claim
9 words
The improvement of claim 1, said sorbent being charcoal.
Claim 4
Dependent Claim
54 words
The improvement of claim 1, said sorbent being selected from the group consisting of charcoal powder as the host substance with zinc hydroxide as the inclusion substance, charcoal powder as the host substance with aluminum hydroxide as the inclusion substance and magnesium trisilicate as the host substance with zinc hydroxide as the inclusion substance.
Claim 5
Dependent Claim
22 words
The improvement of claim 2, said clathrate charcoal consisting essentially of a mixture of zinc sulfate, sodium hydroxide, water and charcoal powder.
Claim 6
Dependent Claim
40 words
The improvement of claim 4, said mixture being present in relative amounts of 3 grams of ZnSO 4 .sup. . 7H 2 O, 15 ml of 1N NaOH, 365 ml of H 2 O and 2.48 grams of charcoal powder.
Claim 7
Dependent Claim
21 words
The improvement of claim 2, said clathrate charcoal consisting essentially of a mixture of alum, sodium hydroxide, water and charcoal powder.
Claim 8
Dependent Claim
10 words
The improvement of claim 1, said sorbent being magnesium trisilicate.
Claim 9
Dependent Claim
62 words
A procedure for separating within a first mixture a bound untagged substance and a bound tagged same substance from unbound substance and unbound tagged same substance comprising adding to said first mixture a compound formed from a mixture of hydrate zinc sulfate, sodium hydroxide, water and charcoal powder to form a second mixture and filtering said second mixture to effect the separation.
Claim 10
Dependent Claim
62 words
A procedure for separating within a first mixture a bound untagged substance and a bound tagged same substance from unbound substance and unbound tagged same substance comprising adding to said first mixture a compound formed from a mixture of hydrate zinc sulfate, sodium hydroxide, water and charcoal powder to form a second mixture and filtering said second mixture to effect the separation.
Claim 11
Dependent Claim
78 words
In an automated system for performing immunoassays in which a bound untagged substance and a bound tagged same substance are separated from unbound substance and unbound tagged same substance within a mixture by selectively absorbing the unbound portions, the improvement comprising: A. adding to the mixture to be separated a clathrate charcoal; B. mixing the clathrate charcoal and the mixture; and C. continuously filtering to obtain the bound untagged substance and bound tagged same substance as a filtrate.
Claim 12
Dependent Claim
78 words
In an automated system for performing immunoassays in which a bound untagged substance and a bound tagged same substance are separated from unbound substance and unbound tagged same substance within a mixture by selectively absorbing the unbound portions, the improvement comprising: A. adding to the mixture to be separated a clathrate charcoal; B. mixing the clathrate charcoal and the mixture; and C. continuously filtering to obtain the bound untagged substance and bound tagged same substance as a filtrate.
Claim 13
Dependent Claim
22 words
The improvement of claim 9, said clathrate charcoal consisting essentially of a mixture of zinc sulfate, sodium hydroxide, water and charcoal powder.
Claim 14
Dependent Claim
40 words
The improvement of claim 10, said mixture being present in the relative amounts of 3 grams of ZnSO 4 .sup.. 7H 2 O, 15 ml of 1N NaOH, 365 ml of H 2 O and 2.48 grams of charcoal powder.
Claim 15
Dependent Claim
49 words
A clathrate charcoal inclusion complex product comprising molecules of charcoal powder as a host substance having a crystal lattice and molecules of an inclusion substance formed by the reaction of hydrate zinc sulfate, sodium hydroxide and water, said inclusion substance retained in said crystal lattice to form said product.
Claim 16
Dependent Claim
49 words
A clathrate charcoal inclusion complex product comprising molecules of charcoal powder as a host substance having a crystal lattice and molecules of an inclusion substance formed by the reaction of hydrate zinc sulfate, sodium hydroxide and water, said inclusion substance retained in said crystal lattice to form said product.
Claim 17
Dependent Claim
43 words
The product of claim 12, said product formed by a mixture being present in the relative amounts of 3 grams ZnSO 4 .sup.. 7H 2 O, 15 ml of 1N NaOH, 365 ml of H 2 O and 2.48 grams of charcoal powder.
Claim 18
Dependent Claim
113 words
In an automated system for performing immunoassays wherein a binding agent is mixed with a tagged substance and an amount of untagged same substance to form a mixture having (i) bound tagged substance, (ii) bound untagged substance, (iii) unbound tagged substance, and (iv) unbound untagged substance, the improvement comprising: A. adding to the mixture a clathrate charcoal; B. allowing the mixture and the clathrate charcoal to remain in contact for an incubation period to permit the absorption of the unbound segments of the mixture by the clathrate charcoal; C. continuously filtering the mixture to obtain the bound components as a filtrate; and D. determining the amount of bound tagged substance in the filtrate.
Claim 19
Dependent Claim
113 words
In an automated system for performing immunoassays wherein a binding agent is mixed with a tagged substance and an amount of untagged same substance to form a mixture having (i) bound tagged substance, (ii) bound untagged substance, (iii) unbound tagged substance, and (iv) unbound untagged substance, the improvement comprising: A. adding to the mixture a clathrate charcoal; B. allowing the mixture and the clathrate charcoal to remain in contact for an incubation period to permit the absorption of the unbound segments of the mixture by the clathrate charcoal; C. continuously filtering the mixture to obtain the bound components as a filtrate; and D. determining the amount of bound tagged substance in the filtrate.
References Cited (Backward Citations)
US1519470A
Cited
US2112931A
Cited
US2470688A
Cited
US3442819A
Cited
US3843775A
Cited
US3666854A
Cited
US3768979A
Cited
US3816076A
Cited
US3896217A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-07-04 | 1981-01-03 | 1981-07-05 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-07-04 | 1985-01-03 | 1985-07-05 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-07-04 | 1989-01-03 | 1989-07-05 | $7700.00 | Window Closed / Lapsed |