Patent US1627468A successfully refreshed from Google Patents.
US4035155A
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Method of high speed scintillation autoradiography
Assignee: University Patents Inc
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Filing: 1975-10-30
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Granted: 1977-07-12
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Expires: 1995-10-30
Abstract
Abstract Autoradiography using high specific activity tritiated thymidine (40-60 curies/millimole) and nuclear track emulsion impregnated with a liquid scintillator provides a very rapid method to determine labelling index and growth fraction of cell suspensions. Low temperature (-20° to -85° C) emulsion exposure for 20 - 60 minutes is sufficient for excellent labelling, thus allowing rapid clinical decision making. Exposure times for research techniques requiring very low energy labelling can be considerably shortened, e.g., from 6 months to 2 weeks or less. This technique therefore has broad applicability to biology and medicine.
Inventors
- 👤 Brian G. M. Durie
- 👤 Sydney E. Salmon
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 20:22
CPC Classifications
G; G01; G01T; G01T1/00; G01T1/16; G01T1/20; G01T1/204; C; C12; C12Q; C12Q1/00; C12Q1/68; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/58; G01N33/60; G01T1/29; G01T1/2914; G01T1/2921; G01T1/2942
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
157 words
In a method of measuring physiological changes in tissue which comprises labeling an essential cell constituent with a radioactive isotope by incubating cells with a radiolabeled cell constituent selected from the group consisting of nucleic acids, enzymes, proteins and hormones, which contain a radioisotope as a structural atom thereof, mixing said labeled cells with a liquid scintillator on the surface of a silver halide photographic slide, exposing the silver halide to emissions from the radioisotope, thereby activating silver ions on the slide, developing the slide to produce silver crystals thereon, and determining the relative number of silver crystals as a measure of the radioactivity of the cells and the physiological activity thereof, the improvement wherein the radioactive nucleic acid, enzyme, protein or hormone component each has a specific activity of at least 40 curies per millimole and the silver halide is exposed to the radioactive nucleic acid or enzyme at a temperature not greater than -20° C.
Claim 2
Dependent Claim
157 words
In a method of measuring physiological changes in tissue which comprises labeling an essential cell constituent with a radioactive isotope by incubating cells with a radiolabeled cell constituent selected from the group consisting of nucleic acids, enzymes, proteins and hormones, which contain a radioisotope as a structural atom thereof, mixing said labeled cells with a liquid scintillator on the surface of a silver halide photographic slide, exposing the silver halide to emissions from the radioisotope, thereby activating silver ions on the slide, developing the slide to produce silver crystals thereon, and determining the relative number of silver crystals as a measure of the radioactivity of the cells and the physiological activity thereof, the improvement wherein the radioactive nucleic acid, enzyme, protein or hormone component each has a specific activity of at least 40 curies per millimole and the silver halide is exposed to the radioactive nucleic acid or enzyme at a temperature not greater than -20° C.
Claim 3
Dependent Claim
10 words
The method of claim 1 wherein the radioisotope is tritium.
Claim 4
Dependent Claim
15 words
The method of claim 2 wherein the nucleic acid is ribonucleic acid or deoxyribonucleic acid.
Claim 5
Dependent Claim
16 words
The method of claim 3 wherein the liquid scintillator is a combination of 2,5-diphenyloxazole and 1,4-bis-(4-methyl-5-phenyloxazol-2-yl)benzene.
Claim 6
Dependent Claim
17 words
The method of claim 4 wherein the radioactive nucleic acid component is tritiated thymidine or tritiated uridine.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-07-11 | 1981-01-10 | 1981-07-12 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-07-11 | 1985-01-10 | 1985-07-12 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-07-11 | 1989-01-10 | 1989-07-12 | $7700.00 | Window Closed / Lapsed |