US4035236A
Expired / Lapsed
US
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Process for preparing 9α-hydroxyandrostenedione
Assignee: Pharmacia and Upjohn Co
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Filing: 1975-10-24
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Granted: 1977-07-12
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Expires: 1995-10-24
Abstract
Abstract Mutants which are used in a novel microbiological process to selectively degrade steroids having 17-alkyl side chains of from 2 to 10 carbon atoms, inclusive, to 9α-hydroxyandrostenedione (9α-OH AD). 9α-OH AD is a valuable intermediate to make useful steroids.
Inventors
- 👤 Merle G. Wovcha
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 11:31
CPC Classifications
C; C07; C07J; C07J1/00; C07J1/0003; C07J1/0018; C07J1/0022; C07J1/0011; C12; C12P; C12P33/00; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/83; Y10S435/863; Y10S435/866; Y10S435/867; Y10S435/868; Y10S435/869; Y10S435/872; Y10S435/88; Y10S435/886
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
39 words
A process for preparing 9α-hydroxyandrostenedione which comprises cultivating Mycobacterium fortuitum NRRL B-8119 in an aqueous nutrient medium under aerobic conditions in the presence of a steroid containing from 2 to 10 carbon atoms, inclusive, in the 17-alkyl side chain.
Claim 2
Dependent Claim
39 words
A process for preparing 9α-hydroxyandrostenedione which comprises cultivating Mycobacterium fortuitum NRRL B-8119 in an aqueous nutrient medium under aerobic conditions in the presence of a steroid containing from 2 to 10 carbon atoms, inclusive, in the 17-alkyl side chain.
Claim 3
Dependent Claim
11 words
A process, according to claim 1, wherein said steroid is sitosterol.
Claim 4
Dependent Claim
11 words
A process, according to claim 1, wherein said steroid is cholesterol.
Claim 5
Dependent Claim
11 words
A process, according to claim 1, wherein said steroid is stigmasterol.
Claim 6
Dependent Claim
11 words
A process, according to claim 1, wherein said steroid is campesterol.
Claim 7
Dependent Claim
47 words
A process for preparing 9α-hydroxyandrostenedione which comprises cultivating Mycobacterium fortuitum NRRL B-8119 in an aqueous nutrient medium under aerobic conditions in the presence of a mixture of two or more steroids wherein each steroid contains from 2 to 10 carbon atoms, inclusive, in the 17-alkyl side chain.
Claim 8
Dependent Claim
47 words
A process for preparing 9α-hydroxyandrostenedione which comprises cultivating Mycobacterium fortuitum NRRL B-8119 in an aqueous nutrient medium under aerobic conditions in the presence of a mixture of two or more steroids wherein each steroid contains from 2 to 10 carbon atoms, inclusive, in the 17-alkyl side chain.
Claim 9
Dependent Claim
22 words
A process, according to claim 6, wherein said steroid mixture is selected from the group consisting of sitosterol, cholesterol, stigmasterol, and campesterol.
Claim 10
Dependent Claim
83 words
A process for preparing 9α-hydroxyandrostenedione which comprises cultivating a mutant microorganism selected from the group consisting of Arthrobacter, Bacillus, Brevibacterium, Corynebacterium, Microbacterium, Protaminobacter, Serratia, and Streptomyces, said mutant being characterized by its ability to selectively degrade steroids having 17-alkyl side chains of from 2 to 10 carbon atoms, inclusive, and accumulate 9α-hydroxyandrostenedione in the fermentation beer, in an aqueous nutrient medium under aerobic conditions in the presence of a steroid containing from 2 to 10 carbon atoms, inclusive, in the 17-alkyl side chain.
Claim 11
Dependent Claim
83 words
A process for preparing 9α-hydroxyandrostenedione which comprises cultivating a mutant microorganism selected from the group consisting of Arthrobacter, Bacillus, Brevibacterium, Corynebacterium, Microbacterium, Protaminobacter, Serratia, and Streptomyces, said mutant being characterized by its ability to selectively degrade steroids having 17-alkyl side chains of from 2 to 10 carbon atoms, inclusive, and accumulate 9α-hydroxyandrostenedione in the fermentation beer, in an aqueous nutrient medium under aerobic conditions in the presence of a steroid containing from 2 to 10 carbon atoms, inclusive, in the 17-alkyl side chain.
Claim 12
Dependent Claim
47 words
A process, according to claim 8, wherein said mutant microorganism is cultivated in an aqueous nutrient medium under aerobic conditions in the presence of a mixture of two or more steroids wherein each steroid contains from 2 to 10 carbon atoms, inclusive, in the 17-alkyl side chain.
Claim 13
Dependent Claim
21 words
A process, according to claim 8, wherein said steroid is selected from the group consisting of sitosterol, cholesterol, stigmasterol, and campesterol.
Claim 14
Dependent Claim
22 words
A process, according to claim 9, wherein said steroid mixture is selected from the group consisting of sitosterol, cholesterol, stigmasterol, and campesterol.
References Cited (Backward Citations)
US3759791A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-07-11 | 1981-01-10 | 1981-07-12 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-07-11 | 1985-01-10 | 1985-07-12 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-07-11 | 1989-01-10 | 1989-07-12 | $7700.00 | Window Closed / Lapsed |