US4042329A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method and device for detecting cholesterol
Assignee: Becton Dickinson and Co
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Filing: 1976-04-14
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Granted: 1977-08-16
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Expires: 1996-04-14
Abstract
Abstract A disposable indicator which is useful for the measurement of chemical substances in biological fluids. The instrument registers the concentration of substance in a given biological fluid with indicia which are directly readable in a convenient notation system (e.g., digital or other symbolic notation). The indicator comprises an improvement over prior art disposable devices which require a comparison of the viewable indication with a color intensity scale to translate the indicia registered to meaningful numerical values or other symbolic notations. The indicator permits the use of chemical reagent concentrations which are at least an order of magnitude greater than those used in prior art, thereby improving stability and drastically lowering the magnitude of interferences.
Inventors
- 👤 Harry T. Hochstrasser
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 14:54
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/60; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/52; G01N33/528; Y; Y10; Y10T; Y10T436/00; Y10T436/14; Y10T436/145555; Y10T436/146666; Y10T436/147777; Y10T436/148888
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
55 words
An indicator for the measurement of cholesterol dissolved in biological fluids, which comprises: a support member; and a plurality of indicating reagents for said cholesterol, each of which is located in a separate zone of said member and at least two of which indicate the presence of a different concentration of said cholesterol in solution.
Claim 2
Dependent Claim
55 words
An indicator for the measurement of cholesterol dissolved in biological fluids, which comprises: a support member; and a plurality of indicating reagents for said cholesterol, each of which is located in a separate zone of said member and at least two of which indicate the presence of a different concentration of said cholesterol in solution.
Claim 3
Dependent Claim
19 words
The indicator of claim 1 wherein said support member is hydrophobic and said zones are formed of bibulous material.
Claim 4
Dependent Claim
31 words
The indicator of claim 1 wherein sid reagents are disposed upon said support member in the shape of numerals corresponding to indicia for the level of substance for which they indicate.
Claim 5
Dependent Claim
19 words
The indicator of claim 1 wherein said reagents are disposed upon said member in the order of their sensitivity.
Claim 6
Dependent Claim
41 words
The indicator of claim 1 wherein said support member is mounted on a stiffening member, said stiffening member having apertures therethrough for viewing said separate zones on the underlying support member, said apertures providing, by their shape, appropriate symbols of indicia.
Claim 7
Dependent Claim
15 words
The indicator of claim 5 wherein said stiffening member is a strip of polymeric material.
Claim 8
Dependent Claim
15 words
The indicator of claim 1 wherein said support member is a transparent or translucent sheet.
Claim 9
Dependent Claim
112 words
An assembly for the measurement of cholesterol dissolved in a biological fluid which comprises: a. an indicator which comprises; a support member; and a plurality of indicating reagents for said cholesterol, each of which is disposed in a separate zone of said member and each of which upon exposure to solution of said cholesterol will indicate visually if a different minimum concentration of said cholesterol is present in said solution, by a darkening thereof, each of said reagents being disposed upon said member; and b. a reticule adapted to overlay said indicator and bearing thereon a reference grid corresponding to the order in which said reagents are disposed on said support member.
Claim 10
Dependent Claim
112 words
An assembly for the measurement of cholesterol dissolved in a biological fluid which comprises: a. an indicator which comprises; a support member; and a plurality of indicating reagents for said cholesterol, each of which is disposed in a separate zone of said member and each of which upon exposure to solution of said cholesterol will indicate visually if a different minimum concentration of said cholesterol is present in said solution, by a darkening thereof, each of said reagents being disposed upon said member; and b. a reticule adapted to overlay said indicator and bearing thereon a reference grid corresponding to the order in which said reagents are disposed on said support member.
Claim 11
Independent Claim
75 words
A method of determining the concentration of cholesterol in a biological solution which comprises; immersing in said solution an indicator which comprises; a support member; and a plurality of indicating reagents for said cholesterol, each of which is located in a separate zone of said member and at least two of which indicate the presence of a different concentration of said cholesterol in solution; removing said indicator from said solution; and observing the indicia registered.
Claim 12
Independent Claim
75 words
A method of determining the concentration of cholesterol in a biological solution which comprises; immersing in said solution an indicator which comprises; a support member; and a plurality of indicating reagents for said cholesterol, each of which is located in a separate zone of said member and at least two of which indicate the presence of a different concentration of said cholesterol in solution; removing said indicator from said solution; and observing the indicia registered.
Claim 13
Dependent Claim
27 words
A method according to claim 9 in which the concentration of the cholesterol dissolved in a fluid is determined by visually counting the number of activated zones.
Claim 14
Dependent Claim
17 words
A method according to claim 10 in which the activated zones are counted in an electromechanical device.
Claim 15
Dependent Claim
55 words
A method according to claim 11 in which the zones are arranged in a linear manner and are counted by moving the indicator strip through the sensing zone of an electromechanical counter operated on the basis of detecting zones by their opacity, reflectance, radioactivity, fluorescence, luminescense, electrical conductivity, or other electromagnetic absorptive or radioactive property.
Claim 16
Dependent Claim
86 words
The indicator of claim 1 wherein said indicating reagents comprise a mixture of cholesterol oxidase, peroxidase, a compound oxidizable by hydrogen peroxide plus peroxidide and reconvertable by a reductant and which is visibly colored in either, but not both, of its reduced and oxidized forms, a reductant for the oxidized form of said compound which is oxidizable, said reductant being colorless to the eye, and a buffer composition which will maintain the pH of said reagents at about 7 in the presence of a biological fluid.
Claim 17
Dependent Claim
44 words
The indicator of claim 13 wherein said reductant is selected from the group consisting of 2,5-dihydroxy benzoic acid, indoxyl sulfate; Uric acid; 3,4-dihydroxymandelic acid; 3-methoxy-4-hydroxymandelic acid; 3,4-dihydroxyphenylacetic acid; 3-methoxy-4-hydroxyphenylacetic acid; 3,4-dihydroxyphenylalanine; 3-methoxy-4-hydroxyphenylalanine; 5-hydroxyindole-3-acetic acid, 5-hydroxyindole-2-carboxylic acid; 5-hydroxytryptophane, creatinine; 2,5-dihydroxyphenylacetic acid, o-hydroxytramine (dopamine); and 5-hydroxytryptamine.
References Cited (Backward Citations)
US2464155A
Cited
US2799167A
Cited
US3036894A
Cited
US3006735A
Cited
US3050373A
Cited
US3139328A
Cited
US3235337A
Cited
US3350278A
Cited
US3411887A
Cited
US3290228A
Cited
US3453180A
Cited
US3814668A
Cited
US3699003A
Cited
US3667916A
Cited
US3810739A
Cited
US3964871A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-08-15 | 1981-02-14 | 1981-08-16 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-08-15 | 1985-02-14 | 1985-08-16 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-08-15 | 1989-02-14 | 1989-08-16 | $7700.00 | Window Closed / Lapsed |