US4048016A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Identification of non-fermentative gram-negative bacteria
Assignee: United States Department of Health and Human Services
•
Filing: 1975-04-25
•
Granted: 1977-09-13
•
Expires: 1995-04-25
Abstract
Abstract A method for identification of non-fermentative Gram-negative bacteria (NFB) consisting of correlating results from a plurality of oxidative alkalinization (OAL) assays in media samples consisting essentially of a minimal-protein medium, an indicator and a substrate and a plurality of oxidative acidification (OAC) assays in media consisting essentially of a minimal-protein medium, an indicator and a substrate. A medium for determining oxidative attack (OA) consists essentially of a minimal-protein medium, an indicator and a substrate.
Inventors
- 👤 Lucy A. Otto
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 14:26
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/02; C12Q1/04; Y; Y10; Y10S; Y10S435/00; Y10S435/81; Y10S435/8215; Y10S435/822; Y10S435/85; Y10S435/874; Y10S435/875; Y10S435/876; Y10S435/877; Y10S435/91
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
61 words
Assay medium for determining oxidative attack (OA) by Gram-negative non-fermentative bacteria (NFB) consisting essentially of an oxidative alkalination (OAL) assay medium containing 0.02 -0.05% by weight of casamino acids, bromthymol blue indicator, a substrate and buffered to pH 6.0 and an oxidative acidification (OAC) medium containing 0.02-0.03% by weight of tryptone, bromthymol blue indicator, a substrate and buffered to pH 6.7.
Claim 2
Dependent Claim
61 words
Assay medium for determining oxidative attack (OA) by Gram-negative non-fermentative bacteria (NFB) consisting essentially of an oxidative alkalination (OAL) assay medium containing 0.02 -0.05% by weight of casamino acids, bromthymol blue indicator, a substrate and buffered to pH 6.0 and an oxidative acidification (OAC) medium containing 0.02-0.03% by weight of tryptone, bromthymol blue indicator, a substrate and buffered to pH 6.7.
Claim 3
Dependent Claim
32 words
The medium of claim 1, wherein the substrate for the OAL medium is selected from the group consisting of phenylalanine, acetamide, nicotinamide, glutamine, allantoin, asparagine and malonate, citrate, tartrate and saccharate salts.
Claim 4
Dependent Claim
25 words
The medium of claim 1, wherein the substrate for the OAC medium is selected from the group consisting of polyalcohols, pentoses, disaccharides, ketoses, and hexoses.
Claim 5
Independent Claim
89 words
A method for the identification of "an unknown" non-fermentative Gram-negative bacteria (NFB) consisting of testing said unknown by the results of a battery of oxidative alkalination (OAL) assays in media samples consisting essentially of a minimal-protein medium containing 0.02-0.05% by weight of casamino acids and buffered to pH 6.0, bromthymol blue indicator and a substrate and a plurality of oxidative acidification (OAC) assays in media samples consisting essentially of a minimal-protein medium containing 0.02-0.03% by weight of tryptone and buffered to pH 6.7, bromthymol blue indicator and a substrate.
Claim 6
Independent Claim
89 words
A method for the identification of "an unknown" non-fermentative Gram-negative bacteria (NFB) consisting of testing said unknown by the results of a battery of oxidative alkalination (OAL) assays in media samples consisting essentially of a minimal-protein medium containing 0.02-0.05% by weight of casamino acids and buffered to pH 6.0, bromthymol blue indicator and a substrate and a plurality of oxidative acidification (OAC) assays in media samples consisting essentially of a minimal-protein medium containing 0.02-0.03% by weight of tryptone and buffered to pH 6.7, bromthymol blue indicator and a substrate.
Claim 7
Dependent Claim
49 words
The method of claim 4, wherein the OAC and OAL assays are carried out in separate compartmented trays, each compartment of which contains minimal-protein medium, bromthymol blue indicator and a different substrate, the OAL substrates being assayed in one tray and the OAC substrates in a second compartmented tray.
Claim 8
Dependent Claim
78 words
The method of claim 4, wherein the substrate for the OAC assays is selected from the group consisting of polyalcohols, pentoses, disaccharides and ketoses and the polyalcohols are glycerol, M-inositol, or D-mannitol; the pentoses are L-arabinose, D-ribose, D-xylose or rhamnose; the disaccharides are cellobiose or lactose; and the ketose is D-fructose; wherein the substrate for the OAL assays is selected from the group consisting of phenylalanine, acetamide, nicotinamide, glutamine, allantoin, asparagine and malonate, citrate, tartrate and saccharate salts.
Claim 9
Dependent Claim
51 words
The method of claim 6, wherein the OAL substrates are assayed in one compartmented tray, each compartment of which contains minimal-protein medium, bromthymol blue indicator and substrate; and wherein the OAC substrates are assayed in a second compartmented tray, each compartment of which contains minimal-protein medium, bromthymol blue indicator and substrate.
Claim 10
Dependent Claim
82 words
A kit for the identification of non-fermentative Gram-negative bacteria (NFB) consisting of a compartmented tray containing media for oxidative alkalination (OAL) assays and a compartmented tray containing media for oxidative acidification (OAC) assays, wherein each compartment contains minimal-protein medium, bromthymol blue indicator and a different substrate, and wherein minimal-protein media for OAL assays contains 0.02-0.05% by weight of casamino acids and is buffered to pH 6.0 and for OAC asays contains 0.02-0.03% by weight of tryptone and is buffered to pH 6.7.
Claim 11
Dependent Claim
82 words
A kit for the identification of non-fermentative Gram-negative bacteria (NFB) consisting of a compartmented tray containing media for oxidative alkalination (OAL) assays and a compartmented tray containing media for oxidative acidification (OAC) assays, wherein each compartment contains minimal-protein medium, bromthymol blue indicator and a different substrate, and wherein minimal-protein media for OAL assays contains 0.02-0.05% by weight of casamino acids and is buffered to pH 6.0 and for OAC asays contains 0.02-0.03% by weight of tryptone and is buffered to pH 6.7.
Claim 12
Dependent Claim
86 words
The kit of claim 8, wherein the compartmented tray for OAL assays contains separate compartments, each of which contains minimal-protein medium, bromthymol blue indicator and a substrate selected from the group consisting of phenylalanine, acetamide, nicotinamide, glutamine, allantoin, asparagine, and malonate, citrate, tartrate and saccharate salts; and wherein the compartmented tray for OAC assays contains separate compartments, each of which contains minimal-protein medium, bromthymol blue indicator and a substrate selected from the group consisting of glycerol, M-inositol, D-mannitol, L-arabinose, D-ribose, D-xylose, rhamnose, cellobiose, lactose and D-fructose.
References Cited (Backward Citations)
US3632478A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-09-12 | 1981-03-14 | 1981-09-13 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-09-12 | 1985-03-14 | 1985-09-13 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-09-12 | 1989-03-14 | 1989-09-13 | $7700.00 | Window Closed / Lapsed |