US4049497A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Production of antibiotics neothramycin A and neothramycin B
Assignee: Microbial Chemistry Research Foundation
•
Filing: 1976-12-06
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Granted: 1977-09-20
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Expires: 1996-12-06
Abstract
Abstract There are disclosed two new antibiotics formerly denominated MC916-A and MC916-B and now called neothramycin A and neothramycin B which are potent inhibitors of the growth of leukemia cells, e.g. Leukema L-1210 cells in mice. They are produced by controlled fermentation of Streptomyces FERM-P 2452 (A.T.C.C. 31123).
Inventors
- 👤 Hamao Umezawa
- 👤 Tomio Takeuchi
- 👤 Masa Hamada
- 👤 Shinichi Kondo
- 👤 Masaaki Ishizuka
- 👤 Hiroshi Naganawa
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 21:34
CPC Classifications
A; A61; A61K; A61K36/00; A61K36/06; C; C07; C07G; C07G11/00; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/886
Claims Analysis
Total Claims: 24
Claim 1
Independent Claim
54 words
The process for producing the antibiotic neothramycin complex which comprises culturing a neothramycin-producing strain of Streptomyces having the identifying characteristics of A.T.C.C. 31123 under submerged aerobic conditions in a nutrient medium containing a carbon source and a nitrogenous nutrient until a substantial amount of neothramycin is produced by said organism in said nutrient medium.
Claim 2
Dependent Claim
54 words
The process for producing the antibiotic neothramycin complex which comprises culturing a neothramycin-producing strain of Streptomyces having the identifying characteristics of A.T.C.C. 31123 under submerged aerobic conditions in a nutrient medium containing a carbon source and a nitrogenous nutrient until a substantial amount of neothramycin is produced by said organism in said nutrient medium.
Claim 3
Dependent Claim
29 words
The process of claim 1 in which the strain of Streptomyces is cultured in a nutrient medium at a temperature in the range of 24° C. to 35° C.
Claim 4
Dependent Claim
36 words
The process of claim 1 in which the strain of Streptomyces is cultured in a nutrient medium at a temperature in the range of 25° C. to 29° C. with the pH from 6 to 8.
Claim 5
Dependent Claim
19 words
The process of claim 1 which includes the further step of recovering the neothramycin complex from the culture medium.
Claim 6
Dependent Claim
56 words
The process of claim 4 in which the neothramycin produced in the cultured broth is extracted and purified by a process which includes at least one process selected from the group consisting of salting-out, solvent precipitation, butanol extraction, dialysis, ultrafiltration, isoelectric precipitation, gel filtration, electrophoresis, electrofocusing and adsorption followed by elution from an ion exchange resin.
Claim 7
Dependent Claim
19 words
The process of claim 1 in which the solution containing neothramycin is stored in a cold or frozen state.
Claim 8
Dependent Claim
13 words
The process of claim 1 in which the solution containing neothramycin is freeze-dried.
Claim 9
Dependent Claim
55 words
The process for producing neothramycin A which comprises culturing a neothramycin A producing strain of Streptomyces having the identifying characteristics of A.T.C.C. 31123 under submerged aerobic conditions in a nutrient medium containing a carbon source and a nitrogenous nutrient until a substantial amount of neothramycin A is produced by said organism in said nutrient medium.
Claim 10
Dependent Claim
55 words
The process for producing neothramycin A which comprises culturing a neothramycin A producing strain of Streptomyces having the identifying characteristics of A.T.C.C. 31123 under submerged aerobic conditions in a nutrient medium containing a carbon source and a nitrogenous nutrient until a substantial amount of neothramycin A is produced by said organism in said nutrient medium.
Claim 11
Dependent Claim
29 words
The process of claim 8 in which the strain of Streptomyces is cultured in a nutrient medium at a temperature in the range of 24° C. to 35° C.
Claim 12
Dependent Claim
36 words
The process of claim 8 in which the strain of Streptomyces is cultured in a nutrient medium at a temperature in the range of 25° C. to 29° C. with the pH from 6 to 8.
Claim 13
Dependent Claim
19 words
The process of claim 8 which includes the further step of recovering the neothramycin A from the culture medium.
Claim 14
Dependent Claim
57 words
The process of claim 11 in which the neothramycin A produced in the cultured broth is extracted and purified by a process which includes at least one process selected from the group consisting of salting-out, solvent precipitation, butanol extraction, dialysis, ultra-filtration, isoelectric precipitation, gel filtration, electrophoresis, electrofocusing and adsorption followed by elution from an ion exchange resin.
Claim 15
Dependent Claim
20 words
The process of claim 8 in which the solution containing neothramycin A is stored in a cold or frozen state.
Claim 16
Dependent Claim
14 words
The process of claim 8 in which the solution containing neothramycin A is freeze-dried.
Claim 17
Dependent Claim
53 words
The process for producing neothramycin B which comprises culturing a neothramycin B-producing strain of Streptomyces having the identifying characteristics of A.T.C.C. 31123 under submerged aerobic conditions in a nutrient medium containing a carbon source and a nitrogenous nutrient until a substantial amount of neothramycin B produced by said organism in said nutrient medium.
Claim 18
Dependent Claim
53 words
The process for producing neothramycin B which comprises culturing a neothramycin B-producing strain of Streptomyces having the identifying characteristics of A.T.C.C. 31123 under submerged aerobic conditions in a nutrient medium containing a carbon source and a nitrogenous nutrient until a substantial amount of neothramycin B produced by said organism in said nutrient medium.
Claim 19
Dependent Claim
29 words
The process of claim 15 in which the strain of Streptomyces is cultured in a nutrient medium at a temperature in the range of 24° C. to 35° C.
Claim 20
Dependent Claim
36 words
The process of claim 25 in which the strain of Streptomyces is cultured in a nutrient medium at a temperature in the range of 25° C. to 29° C. with the pH from 6 to 8.
Claim 21
Dependent Claim
19 words
The process of claim 15 which includes the further step of recovering the neothramycin B from the culture medium.
Claim 22
Dependent Claim
57 words
The process of claim 18 in which the neothramycin B produced in the cultured broth is extracted and purified by a process which includes at least one process selected from the group consisting of salting-out, solvent precipitation, butanol extraction, dialysis, ultra-filtration, isoelectric precipitation, gel filtration, electrophoresis, electrofocusing and adsorption followed by elution from an ion exchange resin.
Claim 23
Dependent Claim
20 words
The process of claim 15 in which the solution containing neothramycin B is stored in a cold or frozen state.
Claim 24
Dependent Claim
14 words
The process of claim 15 in which the solution containing neothramycin B is freeze-dried.
References Cited (Backward Citations)
US3074855A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-09-19 | 1981-03-21 | 1981-09-20 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-09-19 | 1985-03-21 | 1985-09-20 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-09-19 | 1989-03-21 | 1989-09-20 | $7700.00 | Window Closed / Lapsed |