US4059967A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for freezing blood platelets
Assignee: New York Blood Center
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Filing: 1976-02-19
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Granted: 1977-11-29
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Expires: 1996-02-19
Abstract
Abstract A frozen proteinaceous mass comprising blood platelets coated with glycerol-glucose, the frozen mass prepared by a process comprising:
Inventors
- 👤 Arthur W. Rowe
- 👤 George Dayian
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 21:21
CPC Classifications
A; A01; A01N; A01N1/00; A01N1/10; A01N1/12; A01N1/122; A01N1/125; A61; A61K; A61K35/00; A61K35/12; A61K35/14; A61K35/16
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
76 words
A process for preserving blood platelets which comprises: A. contacting said platelets with an aqueous saline solution containg 4 to 7 weight percent glycerol and 2 to 6 weight percent glucose, the glycerol being employed in at least a 5 : 1 volume solution to volume platelet ratio; B. removing supernatant liquid; C. concentrating platelets to about 20 mg platelet protein/ml; D. thereafter freezing said platelets at a rate of at least 20° C per minute.
Claim 2
Dependent Claim
76 words
A process for preserving blood platelets which comprises: A. contacting said platelets with an aqueous saline solution containg 4 to 7 weight percent glycerol and 2 to 6 weight percent glucose, the glycerol being employed in at least a 5 : 1 volume solution to volume platelet ratio; B. removing supernatant liquid; C. concentrating platelets to about 20 mg platelet protein/ml; D. thereafter freezing said platelets at a rate of at least 20° C per minute.
Claim 3
Dependent Claim
36 words
A process according to claim 1 wherein a mass results from step A and between steps A and B the resultant mass from step A is subjected to centrifugation to form a concentrated mass of platelets.
Claim 4
Dependent Claim
23 words
A process according to claim 2 wherein the concentrated mass is disposed in the form of a sheet and frozen in such form.
Claim 5
Dependent Claim
20 words
A process according to claim 3 wherein the platelets are frozen down to a temperature of at least -80° C.
Claim 6
Dependent Claim
21 words
A process according to claim 4 wherein the platelets are thereafter stored in liquid nitrogen at a temperature of -196° C.
Claim 7
Dependent Claim
21 words
A process according to claim 1 wherein the volume to volume ratio of saline solution to platelets is 10-45 : 1.
Claim 8
Dependent Claim
22 words
A process according to claim 1 wherein the salt concentration of said saline solution is 0.8 to 0.95 weight to volume percent.
Claim 9
Dependent Claim
27 words
A process according to claim 1 wherein the platelets treated in step A are in admixture with blood plasma and said platelets are concentrated in said plasma.
Claim 10
Dependent Claim
24 words
A process according to claim 1 wherein the platelet protein concentration is present in an amount of 10 to 20 mg protein per ml.
Claim 11
Dependent Claim
74 words
A process for preserving blood platelets which comprises: A. contacting said platelets with an aqueous saline solution containing 4 to 7 weight percent glycerol and 2 to 6 weight percent glucose in at least 5 : 1 volume solution to volume platelet ratio; B. removing supernatant liquid; C. concentrating platelets to about 10-20 mg platelet protein per ml; and D. thereafter freezing said platelets at a rate of at least 20° C per minute.
Claim 12
Dependent Claim
74 words
A process for preserving blood platelets which comprises: A. contacting said platelets with an aqueous saline solution containing 4 to 7 weight percent glycerol and 2 to 6 weight percent glucose in at least 5 : 1 volume solution to volume platelet ratio; B. removing supernatant liquid; C. concentrating platelets to about 10-20 mg platelet protein per ml; and D. thereafter freezing said platelets at a rate of at least 20° C per minute.
Claim 13
Dependent Claim
67 words
A process for preserving blood platelets which comprises: A. contacting said platelets with an aqueous saline solution containing 4 to 7 weight percent glycerol and 2 to 6 weight percent glucose in at least 5 : 1 volume solution to volume platelet ratio; B. removing supernatant liquid; C. concentrating said platelets; and D. thereafter freezing said platelets at a rate of at least 20° C per minute.
Claim 14
Dependent Claim
67 words
A process for preserving blood platelets which comprises: A. contacting said platelets with an aqueous saline solution containing 4 to 7 weight percent glycerol and 2 to 6 weight percent glucose in at least 5 : 1 volume solution to volume platelet ratio; B. removing supernatant liquid; C. concentrating said platelets; and D. thereafter freezing said platelets at a rate of at least 20° C per minute.
Claim 15
Dependent Claim
56 words
A process for preserving blood platelets which comprises: A. contacting said platelets with an aqueous saline solution containing 4 to 7 weight percent glycerol in at least 5 : 1 volume solution to volume platelet ratio; B. removing supernatant liquid; and C. thereafter freezing said platelets at a rate of at least 20° C per minute.
Claim 16
Dependent Claim
56 words
A process for preserving blood platelets which comprises: A. contacting said platelets with an aqueous saline solution containing 4 to 7 weight percent glycerol in at least 5 : 1 volume solution to volume platelet ratio; B. removing supernatant liquid; and C. thereafter freezing said platelets at a rate of at least 20° C per minute.
Claim 17
Dependent Claim
12 words
A process according to claim 12 wherein said saline solution contains dextrose.
References Cited (Backward Citations)
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-11-28 | 1981-05-30 | 1981-11-29 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-11-28 | 1985-05-30 | 1985-11-29 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-11-28 | 1989-05-30 | 1989-11-29 | $7700.00 | Window Closed / Lapsed |