US4073694A
Expired / Lapsed
US
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Microbiological assay of aminoglycoside antibiotics
Assignee: Johnston Laboratories Inc
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Filing: 1976-04-05
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Granted: 1978-02-14
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Expires: 1996-04-05
Abstract
Abstract A method of measuring the level of an aminoglycoside antibiotic, such as gentamicin, tobramycin or amikacin, in blood serum or plasma comprising incubating an aliquot of blood serum or plasma from a human patient being treated with an aminoglycoside antibiotic in a urea-containing culture medium with a susceptible strain of an adaptive urease producing microorganism corresponding to Proteus rettgeri JLI 03, ATCC No. 31168.
Inventors
- 👤 Dennis Buda
- 👤 Rodney L. Broman
- 👤 John R. Waters
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 16:52
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/02; C12Q1/18; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/873
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
72 words
In a method of determining the concentration of an aminoglycoside antibiotic in blood wherein an aliquot of the liquid portion of blood from a patient being treated with said aminoglycoside antibiotic is incubated in a urea containing test medium with a susceptible strain of an adaptive urease producing microorganism, the improvement comprising using a microorganism having the characteristics of Proteus rettgeri JLI 03, ATCC No. 31168 as the adaptive urease producing microorganism.
Claim 2
Dependent Claim
72 words
In a method of determining the concentration of an aminoglycoside antibiotic in blood wherein an aliquot of the liquid portion of blood from a patient being treated with said aminoglycoside antibiotic is incubated in a urea containing test medium with a susceptible strain of an adaptive urease producing microorganism, the improvement comprising using a microorganism having the characteristics of Proteus rettgeri JLI 03, ATCC No. 31168 as the adaptive urease producing microorganism.
Claim 3
Dependent Claim
13 words
A method as recited in claim 1 wherein said aminoglycoside antibiotic is gentamicin.
Claim 4
Dependent Claim
13 words
A method as recited in claim 1 wherein said aminoglycoside antibiotic is amikacin.
Claim 5
Dependent Claim
13 words
A method as recited in claim 1 wherein said aminoglycoside antibiotic is tobramycin.
Claim 6
Dependent Claim
31 words
A method as recited in claim 1 wherein said test medium comprises 14 C-labeled urea and said method further comprises determining the amount of 14 CO 2 produced during the incubation.
Claim 7
Dependent Claim
23 words
A method as recited in claim 5 wherein the specific activity of said 14 C-labeled urea is less than 0.001 microcuries per micromole.
Claim 8
Dependent Claim
36 words
A method as recited in claim 5 wherein the test medium is acidified after incubation to release 14 CO 2 from the test medium and the evolved 14 CO 2 is measured in the vapor phase.
Claim 9
Dependent Claim
47 words
A method as recited in claim 5 wherein the amount of 14 CO 2 produced during the incubation is compared with that produced from control media containing an aliquot of pooled normal liquid from human blood and known concentrations of aminoglycoside antibiotic processed in the same manner.
Claim 10
Dependent Claim
72 words
A method as recited in claim 6 wherein the initial pH of the test medium lies between about 7.0 and about 8.5, the initial inoculation of microorganism into the test medium comprises from about 10 6 to about 10 8 microorganisms and the incubation is carried out at a temperature lying in the range from about 35° C to about 39° C for a period of time less than about 2 hours.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1981-02-13 | 1981-08-15 | 1982-02-14 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1985-02-13 | 1985-08-15 | 1986-02-14 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1989-02-13 | 1989-08-15 | 1990-02-14 | $7700.00 | Window Closed / Lapsed |