US4122196A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for the manufacture of yeast glycan
Assignee: Anheuser Busch Companies LLC
•
Filing: 1976-08-02
•
Granted: 1978-10-24
•
Expires: 1996-08-02
Abstract
Abstract A process for making a useful product from yeast cell walls is disclosed. The process involves autolysis of whole yeast cells followed by separation of the insoluble cellular debris which is homogenized, extracted, and again separated into solubles and insolubles fractions. The insolubles fraction is edible when purified and increases the viscosity of aqueous solutions while imparting a fat-like mouthfeel to the same. The insolubles fraction is called yeast glycan.
Inventors
- 👤 Ernest Aleck Robbins
- 👤 Robert Dudley Seeley
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 10:04
CPC Classifications
C; C12; C12P; C12P19/00; C12P19/26; C12N; C12N1/00; C12N1/005; C12N1/06; C12N1/063; Y; Y10; Y10S; Y10S435/00; Y10S435/803; Y10S435/8215; Y10S435/911; Y10S435/94; Y10S435/942
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
145 words
A process for the production of yeast glycan comprising the steps of: A. autolyzing live yeast cells, B. separating the autolyzed yeast cells into solubles fraction and an insoluble cellular debris fraction which contains unwanted constituents whereby the debris fraction does not have the desired ability to thicken food systems and is highly flavored, C. comminuting the insoluble cellular debris fraction in an aqueous system, D. extracting said unwanted constituents from the comminuted cellular debris fraction in an aqueous system at a pH from about 3 to about 12, a temperature of about 10° to about 80° C for up to about 60 minutes to yield a solubles portion and an insolubles portion from which said unwanted constituents have been released, E. separating the solubles portion from the insolubles portion of the extracted cellular debris fraction, and F. recovering the insolubles portion as yeast glycan.
Claim 2
Dependent Claim
145 words
A process for the production of yeast glycan comprising the steps of: A. autolyzing live yeast cells, B. separating the autolyzed yeast cells into solubles fraction and an insoluble cellular debris fraction which contains unwanted constituents whereby the debris fraction does not have the desired ability to thicken food systems and is highly flavored, C. comminuting the insoluble cellular debris fraction in an aqueous system, D. extracting said unwanted constituents from the comminuted cellular debris fraction in an aqueous system at a pH from about 3 to about 12, a temperature of about 10° to about 80° C for up to about 60 minutes to yield a solubles portion and an insolubles portion from which said unwanted constituents have been released, E. separating the solubles portion from the insolubles portion of the extracted cellular debris fraction, and F. recovering the insolubles portion as yeast glycan.
Claim 3
Dependent Claim
24 words
The process of claim 1 wherein the yeast is selected from the group consisting of baker' s yeast, brewer' s yeast, and mixtures thereof.
Claim 4
Dependent Claim
33 words
The process of claim 1 wherein the autolysis is at about 6% to about 20% solids and a temperature of about 20° to about 60° C for about 10 to about 72 hours.
Claim 5
Dependent Claim
21 words
The process of claim 3 wherein the autolysis occurs in the presence of about 1% of a salt or acetate ester.
Claim 6
Dependent Claim
11 words
The process of claim 4 wherein the ester is ethyl acetate.
Claim 7
Dependent Claim
16 words
The process of claim 1 wherein the comminution of the insoluble cellular material is by homogenization.
Claim 8
Dependent Claim
59 words
The process of claim 6 wherein the homogenization is at a pH of about 2 to about 12, a temperature of about 10° to about 80° C., a solids content of about 3% to about 16%, a pressure of about 5000 to about 15,000 psig, and the material is passed through the homogenizer about one to about six times.
Claim 9
Dependent Claim
32 words
The process of claim 1 wherein the extraction of the comminuted cellular material is at a solids content of about 8% to about 10% for about 2 minutes to about 60 minutes.
Claim 10
Dependent Claim
45 words
The process of claim 1 wherein the recovered glycan has a composition of about 5% to about 20% protein, about 0.1% to about 5% lipid, about 1% to about 3% nucleic acid, about 0.5 to about 3% ash, and about 60% to about 90% carbohydrate.
Claim 11
Dependent Claim
29 words
The process of claim 9 wherein the glycan has about 70% to about 100% fragmented cells, and about 0% to about 30% whole cells containing methylene blue stainable material.
Claim 12
Dependent Claim
18 words
The process of claim 1 wherein the extraction of the comminuted cellular material is at an alkaline pH.
Claim 13
Dependent Claim
23 words
The process of claim 1 wherein the extraction of the comminuted cellular material is at a pH of about 9.5 to about 12.
Claim 14
Dependent Claim
169 words
A process for the production of yeast glycan comprising the steps of: A. autolyzing live yeast cells, B. separating the autolyzed yeast cells into solubles fraction and an insoluble cellular debris fraction which contains unwanted constituents whereby the debris fraction does not have the desired ability to thicken food systems and is highly flavored, C. comminuting the insoluble cellular debris fraction in an aqueous system at a pH of about 2 to about 12 and a temperature of about 10° to about 80° C and a solids content of about 3% to about 16%, D. extracting said unwanted constituents from the comminuted cellular debris fraction in an aqueous system at an alkaline pH, a temperature of about 10° to about 80° C, for up to about 60 minutes to yield a solubles portion and an insolubles portion from which said unwanted constituents have been released, E. separating the solubles portion from the insolubles portion of the extracted cellular debris fraction, and F. recovering the insolubles portion as yeast glycan.
Claim 15
Dependent Claim
169 words
A process for the production of yeast glycan comprising the steps of: A. autolyzing live yeast cells, B. separating the autolyzed yeast cells into solubles fraction and an insoluble cellular debris fraction which contains unwanted constituents whereby the debris fraction does not have the desired ability to thicken food systems and is highly flavored, C. comminuting the insoluble cellular debris fraction in an aqueous system at a pH of about 2 to about 12 and a temperature of about 10° to about 80° C and a solids content of about 3% to about 16%, D. extracting said unwanted constituents from the comminuted cellular debris fraction in an aqueous system at an alkaline pH, a temperature of about 10° to about 80° C, for up to about 60 minutes to yield a solubles portion and an insolubles portion from which said unwanted constituents have been released, E. separating the solubles portion from the insolubles portion of the extracted cellular debris fraction, and F. recovering the insolubles portion as yeast glycan.
Claim 16
Dependent Claim
23 words
The process of claim 13 wherein the extraction of the comminuted cellular material is at a pH of about 9.5 to about 12.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1981-10-23 | 1982-04-24 | 1982-10-24 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1985-10-23 | 1986-04-24 | 1986-10-24 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1989-10-23 | 1990-04-24 | 1990-10-24 | $7700.00 | Window Closed / Lapsed |