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US4126416A
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Method for determining the level of LDL cholesterol in blood plasma
Assignee: Boston University
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Filing: 1977-11-21
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Granted: 1978-11-21
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Expires: 1997-11-21
Abstract
Abstract A method for determining the level of LDL cholesterol in blood plasma wherein LDL cholesterol is separated from other soluble cholesterol fractions by selectively agglutinating LDL with a plant lectin and subsequently detecting the amount of cholesterol associated with agglutinated LDL.
Inventors
- 👤 Barry D. Sears
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 13:22
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/92
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
56 words
In an assay for determining the LDL cholesterol level in a sample of blood plasma: the improvement comprising isolating LDL from other lipoproteins in said sample of blood plasma by selectively agglutinating LDL with a plant lectin which is a specific agglutinating agent for LDL and thereafter determining the amount of cholesterol in said agglutinated LDL.
Claim 2
Dependent Claim
56 words
In an assay for determining the LDL cholesterol level in a sample of blood plasma: the improvement comprising isolating LDL from other lipoproteins in said sample of blood plasma by selectively agglutinating LDL with a plant lectin which is a specific agglutinating agent for LDL and thereafter determining the amount of cholesterol in said agglutinated LDL.
Claim 3
Dependent Claim
14 words
The improvement of claim 1 wherein said plant lectin comprises Ricinus Communis bean lectin.
Claim 4
Dependent Claim
60 words
An assay for determining the LDL cholesterol level in a sample of blood plasma, comprising: a. agglutinating LDL in said sample of blood plasma with a plant lectin which is a specific agglutinating agent for LDL; b. separating agglutinated LDL from other lipoproteins in said sample of blood plasma; and, c. determining the amount of cholesterol in said agglutinated LDL.
Claim 5
Dependent Claim
60 words
An assay for determining the LDL cholesterol level in a sample of blood plasma, comprising: a. agglutinating LDL in said sample of blood plasma with a plant lectin which is a specific agglutinating agent for LDL; b. separating agglutinated LDL from other lipoproteins in said sample of blood plasma; and, c. determining the amount of cholesterol in said agglutinated LDL.
Claim 6
Dependent Claim
29 words
The assay of claim 3 wherein the amount of agglutinated LDL cholesterol is determined by first relieving agglutination to resolubilize LDL cholesterol and subsequently detecting said resolubilized LDL cholesterol.
Claim 7
Dependent Claim
16 words
The assay of claim 4 wherein said resolubilized LDL cholesterol is detected by an optical method.
Claim 8
Dependent Claim
22 words
The assay of claim 5 wherein said optical method for determining the amount of resolubilized LDL cholesterol in an optical absorbance technique.
Claim 9
Dependent Claim
23 words
The assay of claim 6 wherein the agglutination of LDL cholesterol is relieved by contacting said agglutinating said LDL cholesterol with a sugar.
Claim 10
Dependent Claim
12 words
The assay of claim 7 wherein said sugar comprises galactose or lactose.
Claim 11
Dependent Claim
14 words
The assay of claim 8 wherein said plant lectin comprises Ricinus Communis bean lectin.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1981-11-20 | 1982-05-22 | 1982-11-21 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1985-11-20 | 1986-05-22 | 1986-11-21 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1989-11-20 | 1990-05-22 | 1990-11-21 | $7700.00 | Window Closed / Lapsed |