US4141965A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Assay of immune complexes
Assignee: National Research Development Corp UK
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Filing: 1977-09-07
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Granted: 1979-02-27
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Expires: 1997-09-07
Abstract
Abstract A method for the estimation of an immune complex and analysis of a constituent thereof, comprising a latex particle agglutination test, in which a sample comprising immune complex is incubated with coated latex particles and IgM antibodies, the latex particles coated with said complex constituent and the IgM antibodies being low affinity non-human antibodies to said complex consituent. Also a kit for use in the method of the invention comprising low affinity non-human IgM antibodies to the constituent of complex undergoing analysis, and latex particles coated with said constituent.
Inventors
- 👤 John F. Soothill
- 👤 Roland J. Levinsky
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 10:17
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/564; Y; Y10; Y10S; Y10S436/00; Y10S436/821
Claims Analysis
Total Claims: 18
Claim 1
Independent Claim
68 words
A method for the estimation of a human immune complex and analysis of a constituent thereof, comprising a latex particle agglutination test, in which a sample comprising human physiological fluid is incubated with coated latex particles and IgM antibodies, wherein said latex particles are coated with said human complex constituent and said IgM antibodies are low affinity antibodies raised in a non-human animal against said human complex constituent.
Claim 2
Independent Claim
68 words
A method for the estimation of a human immune complex and analysis of a constituent thereof, comprising a latex particle agglutination test, in which a sample comprising human physiological fluid is incubated with coated latex particles and IgM antibodies, wherein said latex particles are coated with said human complex constituent and said IgM antibodies are low affinity antibodies raised in a non-human animal against said human complex constituent.
Claim 3
Dependent Claim
17 words
A method according to claim 1, in which the complex constituent is an immunoglobulin constituent of complex.
Claim 4
Dependent Claim
17 words
A method according to claim 1, in which the complex constituent is an antigenic constituent of complex.
Claim 5
Dependent Claim
14 words
A method according to claim 1, which comprises decomplementing the sample prior to incubation.
Claim 6
Dependent Claim
28 words
A method according to claim 4, in which the sample is decomplemented by treatment with EDTA to release C1q which is then removed by contacting with an immuno-absorbent.
Claim 7
Dependent Claim
22 words
A method according to claim 1, in which, subsequent to incubation, the inhibition of agglutination is monitored by a slide agglutination technique.
Claim 8
Dependent Claim
25 words
A method according to claim 1, in which, subsequent to incubation, the inhibition of agglutination is monitored by means of an electronic particle counting apparatus.
Claim 9
Dependent Claim
17 words
A method according to claim 7, in which the apparatus is programmed to count unagglutinated particles only.
Claim 10
Dependent Claim
29 words
A latex particles product comprising latex particles coated with an immune complex constituent, wherein said constituent is linked to said latex particles by means of a DNP coupling group.
Claim 11
Dependent Claim
29 words
A latex particles product comprising latex particles coated with an immune complex constituent, wherein said constituent is linked to said latex particles by means of a DNP coupling group.
Claim 12
Dependent Claim
17 words
A latex particle product according to claim 9 wherein said complex substituent is a biologically active material.
Claim 13
Dependent Claim
32 words
A process for the coating of latex particles with complex constituents, which comprises substituting said constituent with a DNP coupling group and thereafter linking said DNP substituted constituent to said latex particles.
Claim 14
Dependent Claim
32 words
A process for the coating of latex particles with complex constituents, which comprises substituting said constituent with a DNP coupling group and thereafter linking said DNP substituted constituent to said latex particles.
Claim 15
Dependent Claim
19 words
A process according to claim 11, in which the complex constituent is an immunoglobulin of class IgM or IgA.
Claim 16
Dependent Claim
23 words
A process for the production of latex particles according to claim 11 wherein said DNP substituted complex constituent is a biologically active material.
Claim 17
Independent Claim
29 words
A method for decomplementing a fluid containing biological material, in which the fluid is treated with EDTA to release C1q which is then removed by treatment with an immuno-absorbent.
Claim 18
Independent Claim
29 words
A method for decomplementing a fluid containing biological material, in which the fluid is treated with EDTA to release C1q which is then removed by treatment with an immuno-absorbent.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1982-02-26 | 1982-08-28 | 1983-02-27 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1986-02-26 | 1986-08-28 | 1987-02-27 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1990-02-26 | 1990-08-28 | 1991-02-27 | $7700.00 | Window Closed / Lapsed |