US4147514A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Test means and method for detecting ketone bodies
Assignee: Bayer Corp
•
Filing: 1977-11-21
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Granted: 1979-04-03
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Expires: 1997-11-21
Abstract
Abstract Test means, method of making a test device and process for detection of ketone bodies are disclosed. More particularly, suitable test means are of the type having a nitroprusside in combination with at least one inorganic salt of a metal selected from the group of magnesium and calcium. Optionally, at least one primary amine is combined therewith. The compositions are advantageously incorporated with a carrier, such as a matrix or tablet, to provide a test device. The metal salt stabilizes the nitroprusside in solution at alkaline pH, allowing for a single-dip production method, promotes ionization of ketone bodies, resulting in a shortened reaction time, and stabilizes the resulting chromophoric complex. The chromophoric complex is further stabilized by the primary amine.
Inventors
- 👤 Thomas A. Magers
- 👤 David L. Tabb
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 20:39
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/64; Y; Y10; Y10T; Y10T436/00; Y10T436/20; Y10T436/200833
Claims Analysis
Total Claims: 28
Claim 1
Independent Claim
43 words
A test composition for detection of ketones at alkaline pH which comprises a soluble nitroprusside in combination with a compound to produce said alkaline pH and at least one inorganic salt of a metal selected from the group consisting of magnesium and calcium.
Claim 2
Dependent Claim
43 words
A test composition for detection of ketones at alkaline pH which comprises a soluble nitroprusside in combination with a compound to produce said alkaline pH and at least one inorganic salt of a metal selected from the group consisting of magnesium and calcium.
Claim 3
Dependent Claim
23 words
The test composition of claim 1 wherein the metal salt is selected from the group consisting of MgSO 4 and CaCl 2 .
Claim 4
Dependent Claim
15 words
The test composition of claim 1 wherein there is present a combination of metal salts.
Claim 5
Dependent Claim
16 words
The test composition of claim 3 wherein said combination comprises MgSO 4 and CaCl 2 .
Claim 6
Dependent Claim
12 words
The test composition of claim 1 wherein said nitroprusside is sodium nitroprusside.
Claim 7
Dependent Claim
18 words
A ketone detection device which comprises a carrier matrix and, incorporated therewith, the test composition of claim 1.
Claim 8
Dependent Claim
12 words
The device of claim 6 in which the carrier matrix is bibulous.
Claim 9
Dependent Claim
16 words
A ketone detection device which comprises a tablet incorporated with the test composition of claim 1.
Claim 10
Dependent Claim
26 words
A ketone detection device which comprises a carrier matrix incorporated with a composition comprising sodium nitroprusside, MgSO 4 and a component to produce an alkaline pH.
Claim 11
Dependent Claim
26 words
A ketone detection device which comprises a carrier matrix incorporated with a composition comprising sodium nitroprusside, MgSO 4 and a component to produce an alkaline pH.
Claim 12
Dependent Claim
23 words
A process for the preparation of a ketone test device which comprises incorporating a carrier matrix with the test composition of claim 1.
Claim 13
Dependent Claim
20 words
The process of claim 10 wherein said incorporating is impregnating with a solution of said test composition, followed by drying.
Claim 14
Dependent Claim
28 words
A process for detection of ketone in a liquid sample which comprises contacting said sample with the test composition of claim 1 and observing any resultant color formed.
Claim 15
Dependent Claim
27 words
A process for detection of ketone in a liquid sample which comprises contacting said sample with the device of claim 6 and observing any resultant color formed.
Claim 16
Dependent Claim
47 words
A test composition for the detection of ketones at an alkaline pH which comprises a soluble nitroprusside, a component to produce said alkaline pH, at least one inorganic salt of a metal selected from the group consisting of magnesium and calcium and at least one primary amine.
Claim 17
Dependent Claim
47 words
A test composition for the detection of ketones at an alkaline pH which comprises a soluble nitroprusside, a component to produce said alkaline pH, at least one inorganic salt of a metal selected from the group consisting of magnesium and calcium and at least one primary amine.
Claim 18
Dependent Claim
26 words
The test composition of claim 14 wherein the at least one metal salt is selected from the group consisting of MgSO 4 and CaCl 2 .
Claim 19
Dependent Claim
17 words
The test composition of claim 14 wherein the at least one metal salt is MgSO 4 .
Claim 20
Dependent Claim
23 words
The test composition of claim 14 wherein the at least one metal salt comprises the combination of MgSO 4 and CaCl 2 .
Claim 21
Dependent Claim
32 words
The test composition of claim 14 wherein the primary amine is selected from those having a pKa approximately the same as the pH at which the test means is to be used.
Claim 22
Dependent Claim
25 words
The test composition of claim 14 wherein the primary amine is aminomethanesulfonic acid, sulfanilic acid, cysteic acid, cyclohexamine, pyridoxamine, ethylene diamine, picolylamine, m-aminophenol or semicarbazide.
Claim 23
Dependent Claim
13 words
The test composition of claim 19 wherein the primary amine is aminomethanesulfonic acid.
Claim 24
Dependent Claim
12 words
The test composition of claim 14 wherein the nitroprusside is sodium nitroprusside.
Claim 25
Dependent Claim
18 words
A ketone detection device which comprises a carrier matrix and, incorporated therewith, the test composition of claim 14.
Claim 26
Dependent Claim
23 words
A process for the preparation of a ketone test device which comprises incorporating a carrier matrix with the test composition of claim 14.
Claim 27
Dependent Claim
28 words
A process for detection of ketone in a liquid sample which comprises contacting said sample with the test composition of claim 14 and observing any resultant color formed.
Claim 28
Dependent Claim
27 words
A process for detection of ketone in a liquid sample which comprises contacting said sample with the device of claim 22 and observing any resultant color formed.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1982-04-02 | 1982-10-02 | 1983-04-03 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1986-04-02 | 1986-10-02 | 1987-04-03 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1990-04-02 | 1990-10-02 | 1991-04-03 | $7700.00 | Window Closed / Lapsed |