US4147772A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Vaccine stabilizer
Assignee: Merck and Co Inc
•
Filing: 1978-02-23
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Granted: 1979-04-03
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Expires: 1998-02-23
Abstract
Abstract A stabilized lyophlized or liquid live viral vaccine contains a live virus, partially hydrolyzed gelatin, a 6-carbon polyhydric alcohol, Medium 199 and acidic buffer.
Inventors
- 👤 William J. McAleer
- 👤 Henry Z. Markus
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 08:01
CPC Classifications
A; A61; A61K; A61K39/00; A61K39/12; A61K39/155; A61K39/165; C; C12; C12N; C12N7/00; C12N2760/00; C12N2760/00011; C12N2760/18011; C12N2760/18411; C12N2760/18434; C12N2760/18451; Y; Y02; Y02A; Y02A50/00; Y02A50/30
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
85 words
A vaccine comprising an inactivated or attenuated virus and a stabilizer consisting essentially of on a parts by weight basis from about 2 to about 5 parts partially hydrolyzed gelatin having a molecular weight of about 3,000, from about 2 to about 55 parts of sorbitol, mannitol or dulcitol, from about 0.5 to about 1.7 parts of Medium 199 or equivalent cell culture medium and an amount of a physiologically acceptable acidic buffer effective to adjust the pH to from about 6.0 to about 6.5.
Claim 2
Dependent Claim
85 words
A vaccine comprising an inactivated or attenuated virus and a stabilizer consisting essentially of on a parts by weight basis from about 2 to about 5 parts partially hydrolyzed gelatin having a molecular weight of about 3,000, from about 2 to about 55 parts of sorbitol, mannitol or dulcitol, from about 0.5 to about 1.7 parts of Medium 199 or equivalent cell culture medium and an amount of a physiologically acceptable acidic buffer effective to adjust the pH to from about 6.0 to about 6.5.
Claim 3
Dependent Claim
13 words
A vaccine according to claim 1 wherein the 6-carbon polyhydric alcohol is sorbitol.
Claim 4
Dependent Claim
12 words
A vaccine according to claim 1 wherein the buffer is phosphate buffer.
Claim 5
Dependent Claim
26 words
A vaccine according to claim 1 wherein the virus is measles, mumps, rubella, varicella, polio or hepatitis, herpes simplex 1, herpes simplex 2, or combinations thereof.
Claim 6
Dependent Claim
54 words
A vaccine according to claim 1 wherein the stabilizer consists essentially of on a parts by weight basis about 3.6 parts of partially hydrolyzed gelatin, about 3.6 parts of sorbitol, about 1.1 parts of Medium 199 and an amount of phosphate buffer effective to adjust the pH to from about 6.0 to about 6.5.
Claim 7
Dependent Claim
53 words
A vaccine according to claim 1 wherein the stabilizer consists essentially on a parts by weight basis about 3.6 parts of partially hydrolyzed gelatin, about 53 parts of sorbitol, about 1.1 parts of Medium 199 and an amount of phosphate buffer effective to adjust the pH to from about 6.0 to about 6.5.
Claim 8
Dependent Claim
16 words
A stabilized vaccine obtained by reconstituting a lyophilized vaccine containing a stabilizer according to claim 1.
Claim 9
Dependent Claim
16 words
A stabilized vaccine obtained by thawing a frozen vaccine containing a stabilizer according to claim 1.
Claim 10
Dependent Claim
22 words
A vaccine according to claim 1 containing from about 2 to about 12 volumes of stabilizer to about one volume of vaccine.
Claim 11
Dependent Claim
24 words
A method for stabilizing a vaccine comprising adding to the vaccine an amount of stabilizer according to claim 1 effective to stabilize the vaccine.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1982-04-02 | 1982-10-02 | 1983-04-03 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1986-04-02 | 1986-10-02 | 1987-04-03 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1990-04-02 | 1990-10-02 | 1991-04-03 | $7700.00 | Window Closed / Lapsed |