US4154726A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for modifying the cell wall of single-cell microorganisms using periodate ions
Assignee: BP Corp North America Inc
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Filing: 1978-03-20
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Granted: 1979-05-15
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Expires: 1998-03-20
Abstract
Abstract A method for modifying the cell wall of single-cell microorganisms comprises treating the whole cells with a dilute solution of a source of perhalate ions to oxidize the structural polysaccharides in the cell wall without damaging the internal cellular components. If the treated cells are further treated with a dilute basic solution, the cells can be totally dissolved. The protein materials in this solution can then be recovered to obtain a variety of protein products that are whippable, coagulable, and soluble, etc.
Inventors
- 👤 Shingo Kajinami
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 22:39
CPC Classifications
C; C12; C12N; C12N1/00; C12N1/06; A; A23; A23J; A23J1/00; A23J1/18; C12N1/005; Y; Y10; Y10S; Y10S530/00; Y10S530/82; Y10S530/823; Y10S530/824
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
27 words
A method for dissolving single-cell microorganisms comprising treating the cells with a dilute source of periodate ions and dissolving the treated cells in a dilute basic solution.
Claim 2
Independent Claim
27 words
A method for dissolving single-cell microorganisms comprising treating the cells with a dilute source of periodate ions and dissolving the treated cells in a dilute basic solution.
Claim 3
Dependent Claim
19 words
The method of claim 1 wherein the source of periodate ions is a dilute solution of NaIO 4 .
Claim 4
Dependent Claim
18 words
The method of claim 1 wherein proteins within the dissolved cell solution are precipitated by acidification and separated.
Claim 5
Dependent Claim
12 words
The method of claim 1 wherein the single-cell microorganism is a yeast.
Claim 6
Dependent Claim
13 words
The method of claim 1 wherein the single-cell microorganism is Candida utilis yeast.
Claim 7
Independent Claim
51 words
A method for treating Candida utilis yeast cells comprising: (a) treating the yeast cells in a dilute solution of NaIO 4 at a temperature of from about 15° C. to about 40° C. for at least about 7 hours; and (b) partitioning the treated yeast cells from the NaIO 4 solution.
Claim 8
Independent Claim
51 words
A method for treating Candida utilis yeast cells comprising: (a) treating the yeast cells in a dilute solution of NaIO 4 at a temperature of from about 15° C. to about 40° C. for at least about 7 hours; and (b) partitioning the treated yeast cells from the NaIO 4 solution.
Claim 9
Dependent Claim
16 words
The method of claim 6, wherein the partitioned cells are dissolved in a dilute basic solution.
Claim 10
Dependent Claim
22 words
The method of claim 7 wherein the dilute basic solution dissolves the cells at from about 40° C. to about 90° C.
Claim 11
Dependent Claim
30 words
The method of claim 6 wherein the yeast cells are treated with a solution of about 0.5N NaIO 4 at a temperature of about 37° C. for about 24 hours.
Claim 12
Dependent Claim
17 words
The method of claim 9 wherein the partitioned yeast cells are dissolved in about 1N hot NaOH.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1982-05-14 | 1982-11-13 | 1983-05-15 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1986-05-14 | 1986-11-13 | 1987-05-15 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1990-05-14 | 1990-11-13 | 1991-05-15 | $7700.00 | Window Closed / Lapsed |