US4157894A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Production and analysis of ginseng root extract
Assignee: Inverni Della Beffa SpA
•
Filing: 1977-05-27
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Granted: 1979-06-12
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Expires: 1997-05-27
Abstract
Abstract A method for producing a purified extract of ginseng root which comprises contacting an aqueous extract of ginseng root with a solid absorption resin so as to absorb active saponins contained in the extract on the resin and eluting active saponins from the resin. Also a method of analyzing a ginseng extract which comprises subjecting the extract to silylation to convert saponins contained in the extract to trimethylsilyl ethers, separating the ethers by gas-liquid chromatography and detecting the separated ethers.
Inventors
- 👤 Ezio Bombardelli
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 20:51
CPC Classifications
A; A61; A61K; A61K36/00; A61K36/18; A61K36/185; A61K36/25; A61K36/258; Y; Y10; Y10T; Y10T436/00; Y10T436/14; Y10T436/142222; Y10T436/143333; Y10T436/20; Y10T436/200833; Y10T436/25; Y10T436/25375; Y10T436/255
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
58 words
A method for producing a purified extract of ginseng root which comprises contacting an aqueous extract of ginseng root with a solid absorption resin comprising an aromatic polymer selected from the group consisting of polystyrene and phenol-formaldehyde resins so as to absorb active saponins contained in the extract on the resin and eluting active saponins from the resin.
Claim 2
Independent Claim
58 words
A method for producing a purified extract of ginseng root which comprises contacting an aqueous extract of ginseng root with a solid absorption resin comprising an aromatic polymer selected from the group consisting of polystyrene and phenol-formaldehyde resins so as to absorb active saponins contained in the extract on the resin and eluting active saponins from the resin.
Claim 3
Dependent Claim
30 words
A method according to claim 1 in which the aqueous extract is contacted with the solid absorption resin by passing the extract through a column or bed of the resin.
Claim 4
Dependent Claim
22 words
A method according to claim 1 in which the active saponins are eluted using a solvent comprising a lower alcohol as eluent.
Claim 5
Dependent Claim
35 words
A method according to claim 1 in which prior to contacting the extract with the absorption resin the extract is subjected to a preliminary purification treatment comprising contacting the extract with an ion exchange resin.
Claim 6
Independent Claim
56 words
A method of analysing a ginseng extract which comprises subjecting the extract to silylation to convert non-hydrolysed saponins selected from the group consisting of ginsenosides Rb 1 , Rb 2 , Rc, Rd, Re, Rf and Rg 1 contained in the extract to trimethylsilyl ethers, separating the ethers by gas-liquid chromotography and detecting the separated ethers.
Claim 7
Independent Claim
56 words
A method of analysing a ginseng extract which comprises subjecting the extract to silylation to convert non-hydrolysed saponins selected from the group consisting of ginsenosides Rb 1 , Rb 2 , Rc, Rd, Re, Rf and Rg 1 contained in the extract to trimethylsilyl ethers, separating the ethers by gas-liquid chromotography and detecting the separated ethers.
Claim 8
Dependent Claim
25 words
A method according to claim 5 in which the saponins are converted to trimethylsilyl ethers by reaction with a mixture of trimethylchlorosilane, N,O-bis-(trimethylsilyl)-acetamide and trimethylsilylimidazole.
Claim 9
Dependent Claim
24 words
A method according to claim 5 in which the separated ethers are detected using a hydrogen flame ionization detector or a mass spectrographic detector.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1982-06-11 | 1982-12-11 | 1983-06-12 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1986-06-11 | 1986-12-11 | 1987-06-12 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1990-06-11 | 1990-12-11 | 1991-06-12 | $7700.00 | Window Closed / Lapsed |