US4162192A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method for purification of HBs antigen
Assignee: Juridical Foundation
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Filing: 1978-09-28
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Granted: 1979-07-24
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Expires: 1998-09-28
Abstract
Abstract An improved method for purification of HBs antigen comprising passing a partially purified HBs antigen prepared from blood plasma or serum through a column filled with an anion exchanger which may be equilibrated with a buffer solution having a specific ionic strength and pH level, and further passing the resulting effluent through a column filled with a cation exchanger which may be equilibrated with a buffer solution having a specific ionic strength and pH level. The HBs antigen obtained by the present invention is highly purified and is useful for preparing vaccine for preventing Viral Hepatitis Type B.
Inventors
- 👤 Kyosuke Mizuno
- 👤 Atsushi Miyanohara
- 👤 Yoshimitsu Ishihara
- 👤 Nobuya Ohtomo
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 06:08
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/005; A; A61; A61K; A61K39/00; C12; C12N; C12N2730/00; C12N2730/00011; C12N2730/10011; C12N2730/10111; C12N2730/10122
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
34 words
A method for purification of HBs antigen, which comprises passing an HBs antigen-containing solution prepared from blood plasma or serum through an anion exchanger and then passing the resulting effluent through a cation exchanger.
Claim 2
Independent Claim
34 words
A method for purification of HBs antigen, which comprises passing an HBs antigen-containing solution prepared from blood plasma or serum through an anion exchanger and then passing the resulting effluent through a cation exchanger.
Claim 3
Dependent Claim
65 words
A method for purification of HBs antigen according to claim 1, wherein the anion exchanger is previously equilibrated with a buffer solution having an ionic strength of 0.02 to 0.1 and a pH level of 5.0 to 7.0, and the cation exchanger is previously equilibrated with a buffer solution having an ionic strength of 0.02 to 0.14 and a pH level of 4.5 to 6.0.
Claim 4
Dependent Claim
61 words
A method for purification of HBs antigen according to claim 2, wherein the buffer solution for treating the anion exchanger has an ionic strength of 0.4 to 0.06 and a pH level of 5.4 to 5.6, and the buffer solution for treating the cation exchanger has an ionic strength of 0.06 to 0.09 and a pH level of 5.0 and 5.3.
Claim 5
Dependent Claim
57 words
A method for purification of HBs antigen according to claim 2 or 3, wherein the HBs antigen-containing solution is dialyzed against the buffer solution to enable the anion exchanging optimal before passing through the anion exchanger and the effluent is dialyzed against the buffer solution to enable the cation exchanging optimal before passing through the cation exchanger.
References Cited (Backward Citations)
US3976767A
Cited
US4113712A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1982-07-23 | 1983-01-22 | 1983-07-24 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1986-07-23 | 1987-01-22 | 1987-07-24 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1990-07-23 | 1991-01-22 | 1991-07-24 | $7700.00 | Window Closed / Lapsed |