Water soluble carrier-bound penicillinacylase

Claim 2

A water-soluble non-colloidal penicillinacylase composition comprising penicillinacylase covalently bound to a cyanogen halide activated water soluble polysaccharide.

Claim 3

A water-soluble non-colloidal penicillinacylase composition comprising penicillinacylase covalently bound to a cyanogen halide activated polysaccharide selected from the group consisting of dextran, water-soluble starch, levan and carboxymethylcellulose, said composition passing through a filter having a pore diameter of 0.8 to 1.0μ but not through a membrane which is impermeable to molecules of molecular weights of 100,000 or greater.

Claim 4

A water-soluble non-colloidal penicillinacylase composition comprising penicillinacylase covalently bound to a cyanogen halide activated polysaccharide selected from the group consisting of dextran, water-soluble starch, levan and carboxymethylcellulose, said composition passing through a filter having a pore diameter of 0.8 to 1.0μ but not through a membrane which is impermeable to molecules of molecular weights of 100,000 or greater.

Claim 5

A water-soluble penicillinacylase composition according to claim 2 wherein the polysaccharide is dextran.

Claim 6

A water-soluble penicillinacylase composition according to claim 3 wherein the dextran is of a molecular weight of about 500,000.

Claim 7

A water-soluble penicillinacylase composition according to claim 3 wherein the polysaccharide is water-soluble starch.

Claim 8

A water-soluble penicillinacylase composition according to claim 3 wherein the polysaccharide is levan.

Claim 9

A water-soluble penicillinacylase composition according to claim 3 wherein the polysaccharide is carboxymethylcellulose.

Claim 10

A water-soluble penicillinacylase composition according to claim 2 dispersed or entrapped in a water-insoluble carrier.

Claim 11

Process for the preparation of a water-soluble non-colloidal penicillinacylase composition according to claim 2 which comprises allowing penicillinacylase to react in an aqueous medium with the reaction product of said polysaccharide and a cyanogen halide.

Claim 12

The process according to claim 9 wherein said cyanogen halide is cyanogen bromide.

Claim 13

The process according to claim 9 wherein the pH of the solution during reaction is about 8.5.

Claim 14

The process according to claim 11 wherein the reaction temperature is from about 5° C. to about 10° C.

Claim 15

The process according to claim 9 wherein the reaction product of the polysaccharide and a cyanogen halide is that obtained from dextran and cyanogen bromide.

Claim 16

The process according to claim 13 wherein the dextran is of a molecular weight of about 500,000.

Claim 17

The process according to claim 14 wherein about 800 mg of cyanogen bromide are used per gram of dextran.

Claim 18

The process according to claim 9 wherein the reaction product of the polysaccharide and a cyanogen halide is that obtained from water-soluble starch and cyanogen bromide.

Claim 19

The process according to claim 9 wherein the reaction product of the polysaccharide and a cyanogen halide is that obtained from levan and cyanogen bromide.

Claim 20

The process according to claim 9 wherein the reaction product of the polysaccharide and a cyanogen halide is that obtained from carboxymethylcellulose and cyanogen bromide.

Claim 21

In the process for the production of 6-aminopenicillanic acid in which a penicillin is treated with an enzymatic material capable of hydrolytically removing the 6-position side chain, the improvement which comprises using as said enzymatic material a water-soluble non-colloidal penicillinacylase composition comprising penicillinacylase covalently bound to a water-soluble cyanogen halide activated polysaccharide.

Claim 22

In the process for the production of 6-aminopenicillanic acid in which a penicillin is treated with an enzymatic material capable of hydrolytically removing the 6-position side chain, the improvement which comprises using as said enzymatic material a water-soluble non-colloidal penicillinacylase composition comprising penicillinacylase covalently bound to a water-soluble cyanogen halide activated polysaccharide.

Claim 23

The process according to claim 19 wherein said polysaccharide is dextran, water-soluble starch, levan or carboxymethylcellulose.

Claim 24

In the process for the production of 6-amino-penicillanic acid in which a penicillin is treated with an enzymatic material capable of hydrolytically removing the 6-position side chain, the improvement which comprises using as said enzymatic material a water-soluble non-colloidal penicillinacylase composition comprising penicillinacylase covalently bound to a cyanogen halide-activated polysaccharide selected from the group consisting of dextran, water-soluble starch, levan or carboxymethylcellulose, said composition passing through a filter having a pore diameter of 0.8 to 1.0μ but not through a membrane which is impermeable to molecules of molecular weights of 100,000 or greater.

Claim 25

In the process for the production of 6-amino-penicillanic acid in which a penicillin is treated with an enzymatic material capable of hydrolytically removing the 6-position side chain, the improvement which comprises using as said enzymatic material a water-soluble non-colloidal penicillinacylase composition comprising penicillinacylase covalently bound to a cyanogen halide-activated polysaccharide selected from the group consisting of dextran, water-soluble starch, levan or carboxymethylcellulose, said composition passing through a filter having a pore diameter of 0.8 to 1.0μ but not through a membrane which is impermeable to molecules of molecular weights of 100,000 or greater.

Claim 26

The process according to claim 21 wherein the polysaccharide is dextran or water-soluble starch.