US4180499A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Carcinoembryonic antigens
Assignee: Hoffmann La Roche Inc
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Filing: 1978-01-09
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Granted: 1979-12-25
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Expires: 1998-01-09
Abstract
Abstract Antigens associated with carcinomas and adenocarcinomas as well as methods for isolating, identifying and detecting them are disclosed.
Inventors
- 👤 Hans J. Hansen
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 13:42
CPC Classifications
B; B01; B01J; B01J39/00; B01J39/26; A; A61; A61K; A61K39/00; A61K39/0005; B01J47/00; B01J47/014; B01J47/02; B01J47/04; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/575; G01N33/57565; A61K2123/00; Y; Y10; Y10S; Y10S530/00; Y10S530/827; Y10S530/828; Y10S530/859
Claims Analysis
Total Claims: 2
Claim 1
Independent Claim
148 words
A process for producing human carcinoembryonic antigen component B which comprises (a) homogenizing human meconium in a glycoprotein solvent; (b) separating the precipitate and solid particles from the homogenate; (c) dialyzing the supernatant against a polyethylene glycol with an average molecular weight of about 15,000 to 20,000 and a softening point at 60° C.; (d) dissolving the retentate in an aqueous buffer of about pH 7; (e) separating the resulting precipitate; (f) subjecting the resulting supernatant to sequential chromatography on two different gel columns, the first of which is a hydrophilic water-insoluble cross-linked dextran polymer gel and the second of which is an agarose gel having about 6% by weight agarose and a particle size of from 40 to 210 microns; (g) collecting and concentrating the eluates which have a spectrophotometric absorption peak of wave length of 280 mμ and a molecular weight of about 200,000 to 500,000.
Claim 2
Dependent Claim
148 words
A process for producing human carcinoembryonic antigen component B which comprises (a) homogenizing human meconium in a glycoprotein solvent; (b) separating the precipitate and solid particles from the homogenate; (c) dialyzing the supernatant against a polyethylene glycol with an average molecular weight of about 15,000 to 20,000 and a softening point at 60° C.; (d) dissolving the retentate in an aqueous buffer of about pH 7; (e) separating the resulting precipitate; (f) subjecting the resulting supernatant to sequential chromatography on two different gel columns, the first of which is a hydrophilic water-insoluble cross-linked dextran polymer gel and the second of which is an agarose gel having about 6% by weight agarose and a particle size of from 40 to 210 microns; (g) collecting and concentrating the eluates which have a spectrophotometric absorption peak of wave length of 280 mμ and a molecular weight of about 200,000 to 500,000.
References Cited (Backward Citations)
US3663684A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1982-12-24 | 1983-06-25 | 1983-12-25 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1986-12-24 | 1987-06-25 | 1987-12-25 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1990-12-24 | 1991-06-25 | 1991-12-25 | $7700.00 | Window Closed / Lapsed |