US4197286A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Testosterone derivatives and assay method
Assignee: Southwest Research Institute
•
Filing: 1977-09-27
•
Granted: 1980-04-08
•
Expires: 1997-09-27
Abstract
Abstract An anti-testosterone serum is provided which is highly selective to the measurement of testosterone using radioimmunossay techniques in both male and female plasma without resorting to chromatography even though the plasma may have a high 5 alpha -dihydrotestosterone content. In addition, the high progesterone concentration normally present in female plasma has no effect on the assay of this plasma. The preferred hapten for producing the serum is 17 beta , 19-dihydroxyandrost-4-en-3-one 19-carboxymethyl ether although related compounds as disclosed herein are also useful.
Inventors
- 👤 Pemmaraju N. Rao
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 14:24
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/74; G01N33/743; C; C07; C07J; C07J1/00; C07J1/0003; C07J1/0018; C07J1/0022; C07J17/00; C07K; C07K16/00; C07K16/44; Y; Y10; Y10S; Y10S436/00; Y10S436/804; Y10S436/815; Y10S436/817; Y10S436/823
Claims Analysis
Total Claims: 26
Claim 1
Independent Claim
133 words
A compound selected from the group consisting of: ##STR9## wherein A is oxygen or sulfur; R is an alkyl group of 1 to 10 carbon atoms; and B is one of (i) --OH, --OM where M is an alkali metal or an alkaline earth metal, or (ii) --OY where Y is an alkyl group of 1 to 10 carbon atoms, or (iii) an amide having the formula ##STR10## where P is an alkyl group of 1 to 10 carbon atoms or a substituted aryl group having the general formula ##STR11## with n being from 1 to 10 or a substituted heterocyclic ring with one or more nitrogen atoms in the ring and having the general formula ##STR12## with n being from 1 to 10 and wherein the compounds are in the beta form.
Claim 2
Dependent Claim
133 words
A compound selected from the group consisting of: ##STR9## wherein A is oxygen or sulfur; R is an alkyl group of 1 to 10 carbon atoms; and B is one of (i) --OH, --OM where M is an alkali metal or an alkaline earth metal, or (ii) --OY where Y is an alkyl group of 1 to 10 carbon atoms, or (iii) an amide having the formula ##STR10## where P is an alkyl group of 1 to 10 carbon atoms or a substituted aryl group having the general formula ##STR11## with n being from 1 to 10 or a substituted heterocyclic ring with one or more nitrogen atoms in the ring and having the general formula ##STR12## with n being from 1 to 10 and wherein the compounds are in the beta form.
Claim 3
Dependent Claim
9 words
The compound of claim 1 wherein A is oxygen.
Claim 4
Dependent Claim
11 words
The compound of claim 1 wherein R has one carbon atom.
Claim 5
Dependent Claim
9 words
The compound of claim 1 wherein B is --OH.
Claim 6
Dependent Claim
4 words
17β, 19-dihydroxyandrost-4-en-3-one 19-carboxymethyl ether.
Claim 7
Dependent Claim
4 words
17β, 19-dihydroxyandrost-4-en-3-one 19-carboxymethyl ether.
Claim 8
Dependent Claim
13 words
An immunogen comprising a compound of claim 1 conjugated to an immunological carrier.
Claim 9
Dependent Claim
13 words
An immunogen comprising a compound of claim 2 conjugated to an immunological carrier.
Claim 10
Dependent Claim
13 words
An immunogen comprising a compound of claim 3 conjugated to an immunological carrier.
Claim 11
Dependent Claim
13 words
An immunogen comprising a compound of claim 4 conjugated to an immunological carrier.
Claim 12
Dependent Claim
13 words
An immunogen comprising a compound of claim 5 conjugated to an immunological carrier.
Claim 13
Dependent Claim
13 words
The immunogen of claim 5 wherein the carrier is bovine serum albumin (BSA).
Claim 14
Dependent Claim
14 words
The antibody produced by injecting an immunogen of claim 6 into a host animal.
Claim 15
Dependent Claim
14 words
The antibody produced by injecting an immunogen of claim 7 into a host animal.
Claim 16
Dependent Claim
14 words
The antibody produced by injecting an immunogen of claim 8 into a host animal.
Claim 17
Dependent Claim
14 words
The antibody produced by injecting an immunogen of claim 9 into a host animal.
Claim 18
Dependent Claim
14 words
The antibody produced by injecting an immunogen of claim 10 into a host animal.
Claim 19
Dependent Claim
14 words
The antibody produced by injecting an immunogen of claim 11 into a host animal.
Claim 20
Independent Claim
41 words
A method of making a radioimmunoassay of testosterone in a sample employing radiolabeled testosterone and an antibody, characterized by employing an antibody produced by injecting, into a host animal, a conjugate of 17β, 19-dihydroxyandrost-4-en-3-one 19-carboxymethyl ether and bovine serum albumin (BSA).
Claim 21
Independent Claim
41 words
A method of making a radioimmunoassay of testosterone in a sample employing radiolabeled testosterone and an antibody, characterized by employing an antibody produced by injecting, into a host animal, a conjugate of 17β, 19-dihydroxyandrost-4-en-3-one 19-carboxymethyl ether and bovine serum albumin (BSA).
Claim 22
Dependent Claim
10 words
The method of claim 18 wherein the ether is thioether.
Claim 23
Dependent Claim
13 words
The method of claim 18 wherein the sample is prepared without chromatographic separation.
Claim 24
Independent Claim
65 words
The method of making a radioimmunoassay of testosterone in a sample obtained from a female during her menstrual cycle so that the sample contains a relatively high content of progesterone as compared to the testosterone content comprising the step of conducting said assay using an antibody produced by injecting into a host animal, a conjugate of 17β, 19-dihydroxyandrost-4-en-3-one 19-carboxymethyl ether and bovine serum alubmin (BSA).
Claim 25
Independent Claim
65 words
The method of making a radioimmunoassay of testosterone in a sample obtained from a female during her menstrual cycle so that the sample contains a relatively high content of progesterone as compared to the testosterone content comprising the step of conducting said assay using an antibody produced by injecting into a host animal, a conjugate of 17β, 19-dihydroxyandrost-4-en-3-one 19-carboxymethyl ether and bovine serum alubmin (BSA).
Claim 26
Dependent Claim
13 words
The method of claim 21 wherein the sample is prepared without chromatographic separation.
References Cited (Backward Citations)
US4031117A
Cited
US4085202A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-04-08 | 1983-10-08 | 1984-04-08 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-04-08 | 1987-10-08 | 1988-04-08 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-04-08 | 1991-10-08 | 1992-04-08 | $7700.00 | Window Closed / Lapsed |