US4200436A
Expired / Lapsed
US
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Immunochemical measuring process
Assignee: Mochida Pharmaceutical Co Ltd
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Filing: 1977-09-28
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Granted: 1980-04-29
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Expires: 1997-09-28
Abstract
Abstract Immunochemical process of measuring antigenic substances such as human chorionic gonadotropin, growth hormone, insulin, immunoglobulins using a labeled antibody, which makes a monovalent bond to the antigen, and insolubilized antigen. The labeled monovalent antibody used is a monovalent antibody obtained by digesting the antibody to an antigen to be measured with papain according to Porter's method or by reducing a fragment obtained by digesting the antibody with pepsin according to Peterman's method.
Inventors
- 👤 Ei Mochida
- 👤 Nobuhisa Ogawa
- 👤 Hiroyuki Shinkai
- 👤 Masakatsu Hashimoto
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 16:07
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/563; Y; Y10; Y10S; Y10S436/00; Y10S436/815; Y10S436/817; Y10S436/818
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
94 words
Immunochemical measuring process comprising the steps of (a) reacting an antigen to be measured with labeled monovalent antibody obtained by bonding a labeling agent to an antibody which makes a monovalent bond to the antigen, (b) reacting insolubilized antigen obtained by insolubilizing the same antigen as that to be measured, with the reaction mixture produced in step (a), (c) separating the reaction mixture of step (b) into a solid phase and a liquid phase, and (d) measuring the activity of the labeling agent of the solid phase or liquid phase separated in step (c).
Claim 2
Dependent Claim
94 words
Immunochemical measuring process comprising the steps of (a) reacting an antigen to be measured with labeled monovalent antibody obtained by bonding a labeling agent to an antibody which makes a monovalent bond to the antigen, (b) reacting insolubilized antigen obtained by insolubilizing the same antigen as that to be measured, with the reaction mixture produced in step (a), (c) separating the reaction mixture of step (b) into a solid phase and a liquid phase, and (d) measuring the activity of the labeling agent of the solid phase or liquid phase separated in step (c).
Claim 3
Dependent Claim
41 words
The immunochemical measuring process of claim 1, wherein the monovalent antibody is a papain-digested fragment of the antibody to the antigenic substance to be measured or a pepsin-digested and reduced fragment of the antibody to the antigenic substance to be measured.
Claim 4
Dependent Claim
19 words
The immunochemical measuring process of claim 1, wherein the labeling agent is a radioisotope, enzyme or a fluorescent material.
Claim 5
Dependent Claim
21 words
The immunochemical measuring process of claim 1, wherein the antigenic substance to be measured is a complete antigen or a hapten.
Claim 6
Dependent Claim
18 words
The immunochemical measuring process of claim 1, in which the labeled antibody and the insolubilized antigen are lyophilized.
Claim 7
Dependent Claim
20 words
The immunochemical measuring process of claim 1, wherein the insolubilized antibody is an antigen insolubilized by binding to a carrier.
Claim 8
Dependent Claim
14 words
The immunochemical measuring process of claim 4, wherein the carrier is polysaccharide or plastic.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-04-29 | 1983-10-29 | 1984-04-29 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-04-29 | 1987-10-29 | 1988-04-29 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-04-29 | 1991-10-29 | 1992-04-29 | $7700.00 | Window Closed / Lapsed |