US4229529A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for determining formate and reagent therefor
Assignee: Roche Diagnostics GmbH
•
Filing: 1978-02-23
•
Granted: 1980-10-21
•
Expires: 1998-02-23
Abstract
Abstract Formate, or compounds convertible into formate, is determined by reacting same in the presence of formate dehydrogenase (FDH) and of a hydrogen acceptor, e.g., NAD, wherein the formate dehydrogenase is from Candida boidinii DSM 941, to result in stoichiometric reactions. The process can be used for the removal of formate from solutions containing same.
Inventors
- 👤 Gerhard Michal
- 👤 Rolf Laube
- 👤 Albert Roder
- 👤 Walter Schneider
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 16:16
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/0004; C12N9/0008; C12Q; C12Q1/00; C12Q1/26; C12Q1/32; C12Y; C12Y102/00; C12Y102/01; C12Y102/01002
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
54 words
Process for the determination of a formate compound selected from formate, or a compound convertible into formate, which process comprises reacting said formate compound in the presence of formate dehydrogenase from Candida boidinii DSM 941 and a hydrogen acceptor, and then measuring the NADH formed as a measure of the formate compound initially present.
Claim 2
Dependent Claim
54 words
Process for the determination of a formate compound selected from formate, or a compound convertible into formate, which process comprises reacting said formate compound in the presence of formate dehydrogenase from Candida boidinii DSM 941 and a hydrogen acceptor, and then measuring the NADH formed as a measure of the formate compound initially present.
Claim 3
Dependent Claim
30 words
Process as claimed in claim 1 wherein the hydrogen acceptor is nicotinamide-adenosine-dinucleotide, and the formate compound initially present is measured by optically measuring the reduced dinucleotide formed in said process.
Claim 4
Dependent Claim
21 words
Process as claimed in claim 1 wherein said Candida boidinii DSM 941 has been cultured on methanol as a carbon source.
Claim 5
Dependent Claim
20 words
Process as claimed in claim 1 wherein the reaction is carried out at a pH of from 5.5 to 10.
Claim 6
Dependent Claim
20 words
Process as claimed in claim 4 wherein the reaction is carried out at a pH of from 6.5 to 9.5
Claim 7
Dependent Claim
12 words
Process as claimed in claim 1 wherein said formate compound is oxalate.
Claim 8
Dependent Claim
45 words
Process for the removal of a formate compound selected from formate and compounds convertible into formate which process comprises reacting the composition containing formate in the presence of formate dehydrogenase from Candida boidinii DSM 941 and a hydrogen acceptor, whereby said formate is quantitatively converted.
Claim 9
Dependent Claim
45 words
Process for the removal of a formate compound selected from formate and compounds convertible into formate which process comprises reacting the composition containing formate in the presence of formate dehydrogenase from Candida boidinii DSM 941 and a hydrogen acceptor, whereby said formate is quantitatively converted.
Claim 10
Dependent Claim
58 words
Reagent for the determination of a formate compound selected from formate and compounds convertible into a formate, which reagent comprises formate dehydrogenase from Candida boidinii DSM 941 in an amount sufficient to react with the formate compound to be determined, and a hydrogen acceptor and a buffer in an amount sufficient to quantify the formate to be determined.
Claim 11
Dependent Claim
58 words
Reagent for the determination of a formate compound selected from formate and compounds convertible into a formate, which reagent comprises formate dehydrogenase from Candida boidinii DSM 941 in an amount sufficient to react with the formate compound to be determined, and a hydrogen acceptor and a buffer in an amount sufficient to quantify the formate to be determined.
Claim 12
Dependent Claim
12 words
Reagent as claimed in claim 8 wherein the hydrogen acceptor is nicotinamide-adenosine-dinucleotide.
Claim 13
Dependent Claim
43 words
Reagent as claimed in claim 8 wherein the reagent comprises 0.1 to 10 mMol/liter NAD, 10 to 250 mMol/liter phosphate buffer, and 0.05 to 5 U/ml formate dehydrogenase from Candida boidinii DSM 941, based on, in each case, to total aqueous solution reagent.
Claim 14
Dependent Claim
15 words
Reagent as claimed in claim 8 comprising from 25 to 100 mMol/liters of phosphate buffer.
Claim 15
Dependent Claim
24 words
Reagent as claimed in claim 8 also comprising a stabilizer compound to stabilize at least one of the formate dehydrogenase in the hydrogen acceptor.
Claim 16
Dependent Claim
11 words
Reagent as claimed in claim 8 also comprising a sequestering agent.
Claim 17
Dependent Claim
12 words
Reagent as claimed in claim 8 also comprising a surface active agent.
References Cited (Backward Citations)
DE2013700C3
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-10-21 | 1984-04-21 | 1984-10-21 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-10-21 | 1988-04-21 | 1988-10-21 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-10-21 | 1992-04-21 | 1992-10-21 | $7700.00 | Window Closed / Lapsed |