US4230805A
Expired / Lapsed
US
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Theophylline antigens and antibodies
Assignee: Siemens Healthcare Diagnostics GmbH Germany
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Filing: 1979-01-11
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Granted: 1980-10-28
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Expires: 1999-01-11
Abstract
Abstract Theophylline derivatives are provided for preparing reagents for use in competitive protein binding assays and for use as reagents in competitive protein binding assays. Theophylline is substituted at the 3 position and conjugated to antigens for production of antibodies which specifically recognize theophylline as distinct from structurally similar analogs such as caffeine. Enzyme conjugates are provided which find use for measuring the amount of theophylline in a sample suspected of containing theophylline. A method is provided employing the reagents for the determination of theophylline.
Inventors
- 👤 Prithipal Singh
- 👤 Mae W. Hu
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 06:34
CPC Classifications
C; C07; C07D; C07D473/00; C07D473/02; C07D473/04; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/94; Y; Y10; Y10S; Y10S435/00; Y10S435/961; Y10S435/964; Y10S530/00; Y10S530/806
Claims Analysis
Total Claims: 22
Claim 1
Independent Claim
57 words
A compound of the formula ##STR5## wherein: PAA is a poly(amino acid) of at least about 5,000 molecular weight; n is at least 1 and up to the molecular weight of PAA divided by 1,500; and R is a linking group of from 1 to 12 atoms other than hydrogen which are carbon, oxygen, nitrogen and sulfur.
Claim 2
Dependent Claim
57 words
A compound of the formula ##STR5## wherein: PAA is a poly(amino acid) of at least about 5,000 molecular weight; n is at least 1 and up to the molecular weight of PAA divided by 1,500; and R is a linking group of from 1 to 12 atoms other than hydrogen which are carbon, oxygen, nitrogen and sulfur.
Claim 3
Dependent Claim
19 words
A compound according to claim 1, wherein PAA is an antigen of from about 10,000 to 600,000 molecular weight.
Claim 4
Dependent Claim
31 words
A compound according to claim 1, wherein PAA is an enzyme of from about 10,000 to 300,000 molecular weight and n is in the range of from about 1 to 30.
Claim 5
Dependent Claim
103 words
A compound according to claim 1, wherein R is of the formula ##STR6## wherein: X and X 1 are the same or different and are oxygen, imino or sulfur; T is a bond or a hydrocarbon radical from 1 to 10 carbon atoms; T 1 is a bond, a hydrocarbon radical from 1 to 10 carbon atoms or hydrocarbylamino of from 1 to 10 carbon atoms; Y is a bond, amido or oxy; m and p are integers of from 0 to 1, wherein CX is bonded to nitrogen of the theophylline and CX 1 is bonded to nitrogen of the poly(amino acid).
Claim 6
Dependent Claim
18 words
A compound according to claim 4, wherein m and p are 1, and T and Y are bonds.
Claim 7
Dependent Claim
20 words
A compound according to claim 5, wherein T 1 is an aliphatic group of from 1 to 6 carbon atoms.
Claim 8
Dependent Claim
29 words
A compound according to claim 4, wherein m is 0, T and Y are bonds and T 1 is an aliphatic group of from 1 to 6 carbon atoms.
Claim 9
Dependent Claim
33 words
A compound of the formula ##STR7## wherein: PAA is an antigenic poly(amino acid) of from about 10,000 to 600,000 molecular weight and n is in the range of from about 4 to 250.
Claim 10
Dependent Claim
33 words
A compound of the formula ##STR7## wherein: PAA is an antigenic poly(amino acid) of from about 10,000 to 600,000 molecular weight and n is in the range of from about 4 to 250.
Claim 11
Dependent Claim
11 words
A compound according to claim 8, wherein PAA is bovine γ-globulin.
Claim 12
Dependent Claim
81 words
A compound of the formula ##STR8## wherein: ENZ is an enzyme; n 1 is an integer which on the average is in the range of 1 to the molecular weight of the enzyme divided by about 2,000; R 2 is a bond or linking group; X and X 1 are the same or different and are oxygen, imino or sulfur; m and p are integers of from 0 to 1 and wherein at least one of m or P is 1.
Claim 13
Dependent Claim
81 words
A compound of the formula ##STR8## wherein: ENZ is an enzyme; n 1 is an integer which on the average is in the range of 1 to the molecular weight of the enzyme divided by about 2,000; R 2 is a bond or linking group; X and X 1 are the same or different and are oxygen, imino or sulfur; m and p are integers of from 0 to 1 and wherein at least one of m or P is 1.
Claim 14
Dependent Claim
11 words
A compound according to claim 10, wherein ENZ is a dehydrogenase.
Claim 15
Dependent Claim
34 words
A compound according to claim 11, wherein m is 0 and R 2 is an aliphatic linking group of from 1 to 10 atoms other than hydrogen which are carbon, oxygen, nitrogen and sulfur.
Claim 16
Dependent Claim
34 words
A compound according to claim 10, wherein m is 0 and R 2 is an aliphatic linking group of from 1 to 10 atoms other than hydrogen, which are carbon, oxygen, nitrogen and sulfur.
Claim 17
Dependent Claim
32 words
A compound according to claim 10, wherein m is 0, X 1 is oxygen, p is 1 and R 2 is a saturated aliphatic group of from 1 to 6 carbon atoms.
Claim 18
Dependent Claim
25 words
A compound of the formula ##STR9## wherein: ENZ is an enzyme; and n 1 is on the average in the range of 2 to 16.
Claim 19
Dependent Claim
25 words
A compound of the formula ##STR9## wherein: ENZ is an enzyme; and n 1 is on the average in the range of 2 to 16.
Claim 20
Dependent Claim
12 words
A compound according to claim 15, wherein said enzyme is glucose-6-phosphate dehydrogenase.
Claim 21
Dependent Claim
27 words
An antibody prepared in an immunological response to a compound according to claim 2, and capable of binding to theophylline and said compound according to claim 2.
Claim 22
Dependent Claim
26 words
Antibodies prepared in an immunological response to a compound according to claim 8 and capable of binding to theophylline and said compound according to claim 8.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-10-28 | 1984-04-28 | 1984-10-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-10-28 | 1988-04-28 | 1988-10-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-10-28 | 1992-04-28 | 1992-10-28 | $7700.00 | Window Closed / Lapsed |