US4232001A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Methods and materials for detection of estrophilin
Assignee: University Patents Inc
•
Filing: 1978-09-22
•
Granted: 1980-11-04
•
Expires: 1998-09-22
Abstract
Abstract Antibodies specifically immunologically reactive with estrophilin. Methods and materials for detection and quantification of estrophilin in tissue samples, notably those of human breast cancer tissue.
Inventors
- 👤 Elwood V. Jensen
- 👤 Eugene R. DeSombre
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 06:50
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/575; G01N33/57515; Y; Y10; Y10S; Y10S436/00; Y10S436/811; Y10S436/813; Y10S436/815; Y10S436/817; Y10S530/00; Y10S530/827; Y10S530/828
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
134 words
A specific, immunologically active anti-estrophilin antibody obtained as a serum immunoglobulin component from an animal immunized with estrophilin, and characterized by its donation to the immunoglobulin of the following properties; (1) the ability to precipitate estrophilin from a mixture of estrophilin and said immunoglobulin upon addition of heterologous species antibodies to the immunoglobulin of the serum donor species; (2) the ability to mediate rapid absorption of estrophilin from a fluid by support polymer when said immunoglobulin is linked to the support polymer; (3) the ability of said immunoglobulin to mediate absorption of estrophilin from a fluid by S. aureus protein-A linked to a support polymer; (4) the ability of said immunoglobulin to increase the sedimentation rate of estrophilin; and, (5) the ability of said immunoglobulin to modify elution characteristics of estrophilin on gel filtration.
Claim 2
Dependent Claim
134 words
A specific, immunologically active anti-estrophilin antibody obtained as a serum immunoglobulin component from an animal immunized with estrophilin, and characterized by its donation to the immunoglobulin of the following properties; (1) the ability to precipitate estrophilin from a mixture of estrophilin and said immunoglobulin upon addition of heterologous species antibodies to the immunoglobulin of the serum donor species; (2) the ability to mediate rapid absorption of estrophilin from a fluid by support polymer when said immunoglobulin is linked to the support polymer; (3) the ability of said immunoglobulin to mediate absorption of estrophilin from a fluid by S. aureus protein-A linked to a support polymer; (4) the ability of said immunoglobulin to increase the sedimentation rate of estrophilin; and, (5) the ability of said immunoglobulin to modify elution characteristics of estrophilin on gel filtration.
Claim 3
Dependent Claim
18 words
An immunological reagent comprising the anti-estrophilin antibody of claim 1 in combination with a radiologically detectable marker substance.
Claim 4
Dependent Claim
12 words
The reagent of claim 2 wherein the marker substance is tritiated estradiol.
Claim 5
Dependent Claim
27 words
An immunological reagent comprising the anti-estrophilin antibody of claim 1 and a plurality of immunologically inert particles, said substance providing an immunologically sensitive surface on said particles.
Claim 6
Dependent Claim
36 words
An immunological assay for estrophilin in a tissue sample said assay comprising contacting said sample with the reagent of claim 4 and quantitatively detecting the presence of estrophilin by monitoring agglutination behavior of said reagent particles.
Claim 7
Dependent Claim
17 words
An assay according to claim 5 wherein said tissue sample is a human breast cancer tissue sample.
Claim 8
Independent Claim
64 words
The method for obtaining serum immunoglobin containing a specific anti-estrophilin antibody, said method comprising the steps of: preparing a purified complex of radioisotopic estradiol and estrophilin by incubating estrophilin-containing tissue in the presence of radioisotopic estradiol and isolating the complex from said incubate by precipitation, gel filtration and electrophoresis; inoculating an immunologically active animal with said complex and, isolating serum immunoglobin from said animal.
Claim 9
Independent Claim
64 words
The method for obtaining serum immunoglobin containing a specific anti-estrophilin antibody, said method comprising the steps of: preparing a purified complex of radioisotopic estradiol and estrophilin by incubating estrophilin-containing tissue in the presence of radioisotopic estradiol and isolating the complex from said incubate by precipitation, gel filtration and electrophoresis; inoculating an immunologically active animal with said complex and, isolating serum immunoglobin from said animal.
Claim 10
Dependent Claim
27 words
Anti-estrophilin antibody raised in tissue of animal origin against estrophilin of animal origin, said antibody characterized by being immunologically reactive with estrophilin of human breast cancer tissue.
Claim 11
Dependent Claim
27 words
Anti-estrophilin antibody raised in tissue of animal origin against estrophilin of animal origin, said antibody characterized by being immunologically reactive with estrophilin of human breast cancer tissue.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-11-04 | 1984-05-05 | 1984-11-04 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-11-04 | 1988-05-05 | 1988-11-04 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-11-04 | 1992-05-05 | 1992-11-04 | $7700.00 | Window Closed / Lapsed |